6jtd - Revision history

OCA at 10:39, 27 March 2024

OCA — Wed, 27 Mar 2024 10:39:44 GMT

←Older revision		Revision as of 10:39, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Trollius_chinensis Trollius chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6JTD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trollius_chinensis Trollius chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JTD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [~~http~~://pdbe.org/6jtd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [https://pdbe.org/6jtd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [https://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A4Y5RXX8_9MAGN A0A4Y5RXX8_9MAGN]
-	In this work, we explored the catalytic promiscuity of TcCGT1, a new C-glycosyltransferase (CGT) from the medicinal plant Trollius chinensis. TcCGT1 could efficiently and regio-specifically catalyze 8-C-glycosylation of 36 flavones and other flavonoids, and could also catalyze the O-glycosylation of diverse phenolics. Moreover, the crystal structure of TcCGT1 in complex with uridine diphosphate was determined at 1.85 A resolution. Structural analysis with molecular docking revealed a new model for catalytic mechanism of TcCGT1, which was initiated by substrate spontaneous deprotonation. The spacious binding pocket explains the robust substrate promiscuity, and binding pose of the substrate determines C- or O-glycosylation activity. Site-directed mutagenesis at two residues (I94E and G284K) switched C- to O-glycosylation. This work highlights TcCGT1 as the first plant CGT with a crystal structure and the first flavone 8-C-glycosyltransferase, and provides a basis for protein engineering to design efficient glycosylation biocatalysts for drug discovery.	+
-		+
-	Molecular Characterization and Structural Basis of a Promiscuous C-Glycosyltransferase from Trollius chinensis.,He JB, Zhao P, Hu ZM, Liu S, Kuang Y, Zhang M, Li B, Yun CH, Qiao X, Ye M Angew Chem Int Ed Engl. 2019 Jun 4. doi: ~~10.1002~~/~~anie.201905505. PMID:31163097<ref>PMID:31163097<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6jtd" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]		*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Trollius chinensis]]		[[Category: Trollius chinensis]]
-	[[Category: Yun~~, C H~~]]	+	[[Category: Yun CH]]
-	[[Category: Zhao, P]]	+	[[Category: Zhao P]]
-	~~[[Category: C-glycosyltransferase]]~~	+
-	~~[[Category: Glycosyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:37, 24 July 2019

OCA — Wed, 24 Jul 2019 08:37:31 GMT

←Older revision		Revision as of 08:37, 24 July 2019
Line 3:		Line 3:
	<StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JTD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Trollius_chinensis Trollius chinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JTD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [http://pdbe.org/6jtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [http://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [http://pdbe.org/6jtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [http://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr>
Line 16:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 6jtd" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6jtd" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 21:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Trollius chinensis]]
	[[Category: Yun, C H]]		[[Category: Yun, C H]]
	[[Category: Zhao, P]]		[[Category: Zhao, P]]

OCA at 05:57, 19 June 2019

OCA — Wed, 19 Jun 2019 05:57:30 GMT

←Older revision		Revision as of 05:57, 19 June 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6jtd is ~~ON HOLD~~	+	==Crystal structure of TcCGT1 in complex with UDP==
		+	<StructureSection load='6jtd' size='340' side='right'caption='[[6jtd]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6jtd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JTD FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtd OCA], [http://pdbe.org/6jtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jtd RCSB], [http://www.ebi.ac.uk/pdbsum/6jtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtd ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In this work, we explored the catalytic promiscuity of TcCGT1, a new C-glycosyltransferase (CGT) from the medicinal plant Trollius chinensis. TcCGT1 could efficiently and regio-specifically catalyze 8-C-glycosylation of 36 flavones and other flavonoids, and could also catalyze the O-glycosylation of diverse phenolics. Moreover, the crystal structure of TcCGT1 in complex with uridine diphosphate was determined at 1.85 A resolution. Structural analysis with molecular docking revealed a new model for catalytic mechanism of TcCGT1, which was initiated by substrate spontaneous deprotonation. The spacious binding pocket explains the robust substrate promiscuity, and binding pose of the substrate determines C- or O-glycosylation activity. Site-directed mutagenesis at two residues (I94E and G284K) switched C- to O-glycosylation. This work highlights TcCGT1 as the first plant CGT with a crystal structure and the first flavone 8-C-glycosyltransferase, and provides a basis for protein engineering to design efficient glycosylation biocatalysts for drug discovery.

-	~~Authors: Zhao~~, P., Yun, C.H.	+	Molecular Characterization and Structural Basis of a Promiscuous C-Glycosyltransferase from Trollius chinensis.,He JB, Zhao P, Hu ZM, Liu S, Kuang Y, Zhang M, Li B, Yun CH, Qiao X, Ye M Angew Chem Int Ed Engl. 2019 Jun 4. doi: 10.1002/anie.201905505. PMID:31163097<ref>PMID:31163097</ref>

-	~~Description: Crystal structure~~ of ~~TcCGT1 in complex with UDP~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6jtd" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Yun, C H]]
	[[Category: Zhao, P]]		[[Category: Zhao, P]]
-	[[Category: ~~Yun,~~ C.H]]	+	[[Category: C-glycosyltransferase]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Transferase]]

OCA: Protected "6jtd" [edit=sysop:move=sysop]

OCA — Wed, 17 Apr 2019 04:03:25 GMT

Protected "6jtd" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:03, 17 April 2019

OCA: New page: '''Unreleased structure''' The entry 6jtd is ON HOLD Authors: Zhao, P., Yun, C.H. Description: Crystal structure of TcCGT1 in complex with UDP Category: Unreleased Structures [[Cat...

OCA — Wed, 17 Apr 2019 04:03:23 GMT

New page: '''Unreleased structure''' The entry 6jtd is ON HOLD Authors: Zhao, P., Yun, C.H. Description: Crystal structure of TcCGT1 in complex with UDP Category: Unreleased Structures [[Cat...

New page

'''Unreleased structure'''

The entry 6jtd is ON HOLD

Authors: Zhao, P., Yun, C.H.

Description: Crystal structure of TcCGT1 in complex with UDP
[[Category: Unreleased Structures]]
[[Category: Zhao, P]]
[[Category: Yun, C.H]]