6jtz - Revision history

OCA at 10:19, 22 November 2023

OCA — Wed, 22 Nov 2023 10:19:37 GMT

←Older revision		Revision as of 10:19, 22 November 2023
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	<StructureSection load='6jtz' size='340' side='right'caption='[[6jtz]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='6jtz' size='340' side='right'caption='[[6jtz]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jtz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JTZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jtz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JTZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.797Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>RECQL, RECQ1, RECQL1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jtz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtz OCA], [https://pdbe.org/6jtz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jtz RCSB], [https://www.ebi.ac.uk/pdbsum/6jtz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jtz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtz OCA], [https://pdbe.org/6jtz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jtz RCSB], [https://www.ebi.ac.uk/pdbsum/6jtz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RECQ1_HUMAN RECQ1_HUMAN]] DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.<ref>PMID:15886194</ref> <ref>PMID:7961977</ref> <ref>PMID:8056767</ref>	+	[https://www.uniprot.org/uniprot/RECQ1_HUMAN RECQ1_HUMAN] DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.<ref>PMID:15886194</ref> <ref>PMID:7961977</ref> <ref>PMID:8056767</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~DNA helicase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bose, M]]	+	[[Category: Bose M]]
-	[[Category: Das~~, A K~~]]	+	[[Category: Das AK]]
-	[[Category: Das, T]]	+	[[Category: Das T]]
-	[[Category: Ganguly, A]]	+	[[Category: Ganguly A]]
-	[[Category: Mukhopadhyay, S]]	+	[[Category: Mukhopadhyay S]]
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Dna-binding protein]]~~	+
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Recq1]]~~	+
-	~~[[Category: Zn-binding domain~~]]	+

OCA at 12:40, 24 November 2021

OCA — Wed, 24 Nov 2021 12:40:09 GMT

←Older revision		Revision as of 12:40, 24 November 2021
Line 1:		Line 1:

	==Crystal Structure of hRecQ1_D2-Zn-WH containing mutation on beta-hairpin==		==Crystal Structure of hRecQ1_D2-Zn-WH containing mutation on beta-hairpin==
-	<StructureSection load='6jtz' size='340' side='right'caption='[[6jtz]]' scene=''>	+	<StructureSection load='6jtz' size='340' side='right'caption='[[6jtz]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6JTZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jtz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JTZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6jtz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtz OCA], [~~http~~://pdbe.org/6jtz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6jtz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6jtz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtz ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RECQL, RECQ1, RECQL1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jtz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtz OCA], [https://pdbe.org/6jtz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jtz RCSB], [https://www.ebi.ac.uk/pdbsum/6jtz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/RECQ1_HUMAN RECQ1_HUMAN]] DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.<ref>PMID:15886194</ref> <ref>PMID:7961977</ref> <ref>PMID:8056767</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RECQ1 is the shortest among the five human RecQ helicases comprising of two RecA like domains, a zinc-binding domain and a RecQ C-terminal domain containing the winged-helix (WH). Mutations or deletions on the tip of a beta-hairpin located in the WH domain are known to abolish the unwinding activity. Interestingly, the same mutations on the beta-hairpin of annealing incompetent RECQ1 mutant (RECQ1T1) have been reported to restore its annealing activity. In an attempt to unravel the strand annealing mechanism, we have crystallized a fragment of RECQ1 encompassing D2-Zn-WH domains harbouring mutations on the beta-hairpin. From our crystal structure data and interface analysis, we have demonstrated that an alpha-helix located in zinc-binding domain potentially interacts with residues of WH domain, which plays a significant role in strand annealing activity. We have shown that deletion of the alpha-helix or mutation of specific residues on it restores strand annealing activity of annealing deficient constructs of RECQ1. Our results also demonstrate that mutations on the alpha-helix induce conformational changes and affects DNA stimulated ATP hydrolysis and unwinding activity of RECQ1. Our study, for the first time, provides insight into the conformational requirements of the WH domain for efficient strand annealing by human RECQ1.
		+
		+	Residues at the interface between zinc binding and winged helix domains of human RECQ1 play a significant role in DNA strand annealing activity.,Mukhopadhyay S, Das T, Bose M, Jain CK, Chakraborty M, Mukherjee S, Shikha K, Das AK, Ganguly A Nucleic Acids Res. 2021 Nov 18;49(20):11834-11854. doi: 10.1093/nar/gkab968. PMID:34751402<ref>PMID:34751402</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6jtz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: DNA helicase]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bose M]]	+	[[Category: Bose, M]]
-	[[Category: Das AK]]	+	[[Category: Das, A K]]
-	[[Category: Das T]]	+	[[Category: Das, T]]
-	[[Category: Ganguly A]]	+	[[Category: Ganguly, A]]
-	[[Category: Mukhopadhyay S]]	+	[[Category: Mukhopadhyay, S]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Dna-binding protein]]
		+	[[Category: Helicase]]
		+	[[Category: Recq1]]
		+	[[Category: Zn-binding domain]]

OCA at 06:50, 15 April 2020

OCA — Wed, 15 Apr 2020 06:50:49 GMT

←Older revision		Revision as of 06:50, 15 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6jtz is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of hRecQ1_D2-Zn-WH containing mutation on beta-hairpin==
-		+	<StructureSection load='6jtz' size='340' side='right'caption='[[6jtz]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JTZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JTZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jtz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jtz OCA], [http://pdbe.org/6jtz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jtz RCSB], [http://www.ebi.ac.uk/pdbsum/6jtz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jtz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bose M]]
		+	[[Category: Das AK]]
		+	[[Category: Das T]]
		+	[[Category: Ganguly A]]
		+	[[Category: Mukhopadhyay S]]

OCA at 07:34, 10 May 2019

OCA — Fri, 10 May 2019 07:34:57 GMT

←Older revision		Revision as of 07:34, 10 May 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6jtz is ON HOLD	+	The entry 6jtz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6jtz" [edit=sysop:move=sysop]

OCA — Wed, 17 Apr 2019 04:05:04 GMT

Protected "6jtz" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 17 April 2019

OCA: New page: '''Unreleased structure''' The entry 6jtz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Apr 2019 04:05:02 GMT

New page: '''Unreleased structure''' The entry 6jtz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6jtz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]