6jxr - Revision history

OCA at 10:02, 16 September 2020

OCA — Wed, 16 Sep 2020 10:02:39 GMT

←Older revision		Revision as of 10:02, 16 September 2020
Line 1:		Line 1:

-	==Structure of ~~a protein~~==	+	==Structure of human T cell receptor-CD3 complex==
	<SX load='6jxr' size='340' side='right' viewer='molstar' caption='[[6jxr]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='6jxr' size='340' side='right' viewer='molstar' caption='[[6jxr]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 6jxr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6jxr" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[CD3 3D structures\|CD3 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 02:16, 11 April 2020

OCA — Sat, 11 Apr 2020 02:16:56 GMT

←Older revision		Revision as of 02:16, 11 April 2020
Line 3:		Line 3:
	<SX load='6jxr' size='340' side='right' viewer='molstar' caption='[[6jxr]], [[Resolution\|resolution]] 3.70Å' scene=''>		<SX load='6jxr' size='340' side='right' viewer='molstar' caption='[[6jxr]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6jxr]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXR OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6JXR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6jxr]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JXR FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CD247, CD3Z, T3Z, TCRZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3D, T3D ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3E, T3E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3G, T3G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TRAC, TCRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TRBC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CD247, CD3Z, T3Z, TCRZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3D, T3D ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3E, T3E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3G, T3G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TRAC, TCRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TRBC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6jxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jxr OCA], [http://pdbe.org/6jxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jxr RCSB], [http://www.ebi.ac.uk/pdbsum/6jxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jxr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jxr OCA], [http://pdbe.org/6jxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jxr RCSB], [http://www.ebi.ac.uk/pdbsum/6jxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jxr ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA at 22:21, 6 March 2020

OCA — Fri, 06 Mar 2020 22:21:25 GMT

←Older revision		Revision as of 22:21, 6 March 2020
Line 1:		Line 1:

	==Structure of a protein==		==Structure of a protein==
-	<~~StructureSection~~ load='6jxr' size='340' side='right'caption='[[6jxr]], [[Resolution\|resolution]] 3.70Å' scene=''>	+	<SX load='6jxr' size='340' side='right' viewer='molstar' caption='[[6jxr]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6jxr]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JXR FirstGlance]. <br>		<table><tr><td colspan='2'>[[6jxr]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JXR FirstGlance]. <br>
Line 23:		Line 23:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 11:08, 2 October 2019

OCA — Wed, 02 Oct 2019 11:08:58 GMT

←Older revision		Revision as of 11:08, 2 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The entry ~~6jxr~~ is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structure of a protein==
		+	<StructureSection load='6jxr' size='340' side='right'caption='[[6jxr]], [[Resolution\|resolution]] 3.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6jxr]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JXR FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CD247, CD3Z, T3Z, TCRZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3D, T3D ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3E, T3E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CD3G, T3G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TRAC, TCRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), TRBC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jxr OCA], [http://pdbe.org/6jxr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jxr RCSB], [http://www.ebi.ac.uk/pdbsum/6jxr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jxr ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/CD3D_HUMAN CD3D_HUMAN]] Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:[http://omim.org/entry/608971 608971]]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.<ref>PMID:14602880</ref> [[http://www.uniprot.org/uniprot/CD3Z_HUMAN CD3Z_HUMAN]] Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:[http://omim.org/entry/610163 610163]]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.<ref>PMID:16672702</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TVB65_HUMAN TVB65_HUMAN]] V region of the variable domain of T cell receptor (TR) beta chain that participates in the antigen recognition (PubMed:24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed:25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed:23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed:15040585).<ref>PMID:26875526</ref> <ref>PMID:15040585</ref> <ref>PMID:23524462</ref> <ref>PMID:24600447</ref> <ref>PMID:25493333</ref> [[http://www.uniprot.org/uniprot/CD3E_HUMAN CD3E_HUMAN]] The CD3 complex mediates signal transduction. [[http://www.uniprot.org/uniprot/CD3D_HUMAN CD3D_HUMAN]] The CD3 complex mediates signal transduction. [[http://www.uniprot.org/uniprot/CD3Z_HUMAN CD3Z_HUMAN]] Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering. [[http://www.uniprot.org/uniprot/CD3G_HUMAN CD3G_HUMAN]] The CD3 complex mediates signal transduction. [[http://www.uniprot.org/uniprot/TVAL3_HUMAN TVAL3_HUMAN]] V region of the variable domain of T cell receptor (TR) alpha chain that participates in the antigen recognition (PubMed:24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed:25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed:23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed:15040585).<ref>PMID:15040585</ref> <ref>PMID:23524462</ref> <ref>PMID:24600447</ref> <ref>PMID:25493333</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The alphabeta T cell receptor (TCR), in association with the CD3gammaepsilon-CD3deltaepsilon-CD3zetazeta signalling hexamer, is the primary determinant of T cell development and activation, and of immune responses to foreign antigens. The mechanism of assembly of the TCR-CD3 complex remains unknown. Here we report a cryo-electron microscopy structure of human TCRalphabeta in complex with the CD3 hexamer at 3.7 A resolution. The structure contains the complete extracellular domains and all the transmembrane helices of TCR-CD3. The octameric TCR-CD3 complex is assembled with 1:1:1:1 stoichiometry of TCRalphabeta:CD3gammaepsilon:CD3deltaepsilon:CD3zetazeta. Assembly of the extracellular domains of TCR-CD3 is mediated by the constant domains and connecting peptides of TCRalphabeta that pack against CD3gammaepsilon-CD3deltaepsilon, forming a trimer-like structure proximal to the plasma membrane. The transmembrane segment of the CD3 complex adopts a barrel-like structure formed by interaction of the two transmembrane helices of CD3zetazeta with those of CD3gammaepsilon and CD3deltaepsilon. Insertion of the transmembrane helices of TCRalphabeta into the barrel-like structure via both hydrophobic and ionic interactions results in transmembrane assembly of the TCR-CD3 complex. Together, our data reveal the structural basis for TCR-CD3 complex assembly, providing clues to TCR triggering and a foundation for rational design of immunotherapies that target the complex.

-	~~Authors~~:	+	Structural basis of assembly of the human T cell receptor-CD3 complex.,Dong, Zheng L, Lin J, Zhang B, Zhu Y, Li N, Xie S, Wang Y, Gao N, Huang Z Nature. 2019 Sep;573(7775):546-552. doi: 10.1038/s41586-019-1537-0. Epub 2019 Aug, 28. PMID:31461748<ref>PMID:31461748</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6jxr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
		+	[[Category: Dong, D]]
		+	[[Category: Gao, N]]
		+	[[Category: Huang, Z]]
		+	[[Category: Li, N]]
		+	[[Category: Lin, J]]
		+	[[Category: Wang, Y]]
		+	[[Category: Xie, S]]
		+	[[Category: Zhang, B]]
		+	[[Category: Zheng, J]]
		+	[[Category: Zheng, L]]
		+	[[Category: Zhu, Y]]
		+	[[Category: Immune system]]

OCA at 03:59, 21 August 2019

OCA — Wed, 21 Aug 2019 03:59:26 GMT

←Older revision		Revision as of 03:59, 21 August 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6jxr is ON HOLD	+	The entry 6jxr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6jxr" [edit=sysop:move=sysop]

OCA — Wed, 01 May 2019 07:00:44 GMT

Protected "6jxr" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:00, 1 May 2019

OCA: New page: '''Unreleased structure''' The entry 6jxr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 May 2019 07:00:42 GMT

New page: '''Unreleased structure''' The entry 6jxr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6jxr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]