6k15 - Revision history

OCA at 10:40, 27 March 2024

OCA — Wed, 27 Mar 2024 10:40:47 GMT

←Older revision		Revision as of 10:40, 27 March 2024
Line 3:		Line 3:
	<SX load='6k15' size='340' side='right' viewer='molstar' caption='[[6k15]], [[Resolution\|resolution]] 3.40Å' scene=''>		<SX load='6k15' size='340' side='right' viewer='molstar' caption='[[6k15]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k15]] is a 13 chain structure with sequence from [~~http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c] and [http~~://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae_s288c~~ Saccharomyces cerevisiae ~~s288c~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K15 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6K15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k15]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K15 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6k15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k15 OCA], [~~http~~://pdbe.org/6k15 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6k15 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6k15 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6k15 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k15 OCA], [https://pdbe.org/6k15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k15 RCSB], [https://www.ebi.ac.uk/pdbsum/6k15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k15 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/RSC58_YEAST RSC58_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/RSC4_YEAST RSC4_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton.<ref>PMID:8980231</ref> <ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12183366</ref> <ref>PMID:12072455</ref> <ref>PMID:12697820</ref> [[http://www.uniprot.org/uniprot/RSC9_YEAST RSC9_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit plays a role in transcriptional response to stress. It is involved in both repression and activation of mRNAs regulated by the target of rapamycin (TOR) kinases, and in the synthesis of rRNA.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11931764</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/STH1_YEAST STH1_YEAST]] Catalytic component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is the essential ATPase of the complex. It is a DNA translocase capable of nucleosome remodeling. Required for full expression of early meiotic genes. Essential for mitotic growth and repression of CHA1 expression. Also involved in G2 phase control.<ref>PMID:10025404</ref> <ref>PMID:10320476</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9799253</ref> [[http://www.uniprot.org/uniprot/RSC30_YEAST RSC30_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall. Together with HTL1, LDB7, NPL6, RSC3 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11336698</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:16204215</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/RSC8_YEAST RSC8_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9121424</ref> [[http://www.uniprot.org/uniprot/RSC2_YEAST RSC2_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in meiotic sporulation through regulating IME2 expression, and is also essential for 2-micron plasmid maintenance and for normal REP1 protein localization.<ref>PMID:12024034</ref> <ref>PMID:8980231</ref> <ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12702296</ref> <ref>PMID:12183366</ref> <ref>PMID:12072455</ref> <ref>PMID:12697820</ref> [[http://www.uniprot.org/uniprot/HTL1_YEAST HTL1_YEAST]] Required for cell cycle progression through G2/M transition at temperatures higher than 33 degrees Celsius. Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. When associated with the RSC complex, may act coordinately with PKC1 to regulate G2/M transition. Together with LDB7, NPL6, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:12417720</ref> <ref>PMID:12684875</ref> <ref>PMID:16204215</ref> [[http://www.uniprot.org/uniprot/RSC7_YEAST RSC7_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization.<ref>PMID:16204215</ref> [[http://www.uniprot.org/uniprot/RSC6_YEAST RSC6_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and suppresses formamide sensitivity of the RSC8 mutants.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9685490</ref> [[http://www.uniprot.org/uniprot/RSC3_YEAST RSC3_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall, and also for proper metaphase progression. Together with HTL1, LDB7, NPL6, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11336698</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:16204215</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/SFH1_YEAST SFH1_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and required for cell cycle progression.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9154831</ref>	+	[https://www.uniprot.org/uniprot/RSC7_YEAST RSC7_YEAST] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization.<ref>PMID:16204215</ref>
-	~~<div style="background-color~~:~~#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~The RSC complex remodels chromatin structure and regulates gene transcription~~. ~~We report the cryoEM structure~~ of ~~yeast RSC bound to~~ the ~~nucleosome. RSC is delineated into the ATPase motor, the actin-related-protein~~ (~~ARP~~) ~~module~~, and ~~the substrate-recruitment module (SRM)~~. RSC ~~binds~~ the nucleosome ~~mainly through the motor, with the auxiliary subunit Sfh1 engaging the H2A-H2B acidic patch~~ to ~~enable nucleosome ejection. SRM is organized into three substrate-binding lobes poised to bind their respective nucleosomal epitopes~~. The ~~relative orientations of the SRM~~ and ~~the motor on the~~ nucleosome ~~explain the directionality of~~ DNA translocation and ~~promoter nucleosome repositioning by RSC~~. ~~Together~~, ~~our findings shed light on~~ RSC ~~assembly~~ and ~~functionality~~, ~~and provide~~ a ~~framework to understand~~ the ~~mammalian homologs BAF/PBAF and the Sfh1 ortholog INI1/BAF47~~, ~~which are frequently mutated~~ in ~~cancers~~.	+

-	Structure of the RSC complex bound to the nucleosome.,Ye Y, Wu H, Chen K, Clapier CR, Verma N, Zhang W, Deng H, Cairns BR, Gao N, Chen Z Science. 2019 Oct 31. pii: science.aay0033. doi: 10.1126/science.aay0033. PMID:31672915<ref>PMID:31672915</ref>	+	==See Also==
-		+	*[[Helicase 3D structures\|Helicase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 6k15" style~~=~~"background-color:#fffaf0;"></div>~~	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: DNA helicase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Saccharomyces cerevisiae ~~s288c~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Cairns, B]]	+	[[Category: Cairns B]]
-	[[Category: Chen~~, K J~~]]	+	[[Category: Chen KJ]]
-	[[Category: Chen~~, Z C~~]]	+	[[Category: Chen ZC]]
-	[[Category: Gao, N]]	+	[[Category: Gao N]]
-	[[Category: Verma, N]]	+	[[Category: Verma N]]
-	[[Category: Wu, H]]	+	[[Category: Wu H]]
-	[[Category: Ye~~, Y P]]~~	+	[[Category: Ye YP]]
-	~~[[Category: Chromatin remodeler]]~~	+
-	~~[[Category: Dna binding protein]]~~	+
-	~~[[Category: Swi/snf family~~]]	+

OCA at 02:18, 11 April 2020

OCA — Sat, 11 Apr 2020 02:18:20 GMT

←Older revision		Revision as of 02:18, 11 April 2020
Line 3:		Line 3:
	<SX load='6k15' size='340' side='right' viewer='molstar' caption='[[6k15]], [[Resolution\|resolution]] 3.40Å' scene=''>		<SX load='6k15' size='340' side='right' viewer='molstar' caption='[[6k15]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k15]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K15 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6K15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k15]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K15 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K15 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6k15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k15 OCA], [http://pdbe.org/6k15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k15 RCSB], [http://www.ebi.ac.uk/pdbsum/6k15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k15 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k15 OCA], [http://pdbe.org/6k15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k15 RCSB], [http://www.ebi.ac.uk/pdbsum/6k15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k15 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 22:21, 6 March 2020

OCA — Fri, 06 Mar 2020 22:21:57 GMT

←Older revision		Revision as of 22:21, 6 March 2020
Line 1:		Line 1:

	==RSC substrate-recruitment module==		==RSC substrate-recruitment module==
-	<~~StructureSection~~ load='6k15' size='340' side='right'caption='[[6k15]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<SX load='6k15' size='340' side='right' viewer='molstar' caption='[[6k15]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6k15]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K15 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6k15]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K15 FirstGlance]. <br>
Line 9:		Line 9:
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/RSC58_YEAST RSC58_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/RSC4_YEAST RSC4_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton.<ref>PMID:8980231</ref> <ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12183366</ref> <ref>PMID:12072455</ref> <ref>PMID:12697820</ref> [[http://www.uniprot.org/uniprot/RSC9_YEAST RSC9_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit plays a role in transcriptional response to stress. It is involved in both repression and activation of mRNAs regulated by the target of rapamycin (TOR) kinases, and in the synthesis of rRNA.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11931764</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/STH1_YEAST STH1_YEAST]] Catalytic component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is the essential ATPase of the complex. It is a DNA translocase capable of nucleosome remodeling. Required for full expression of early meiotic genes. Essential for mitotic growth and repression of CHA1 expression. Also involved in G2 phase control.<ref>PMID:10025404</ref> <ref>PMID:10320476</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9799253</ref> [[http://www.uniprot.org/uniprot/RSC30_YEAST RSC30_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall. Together with HTL1, LDB7, NPL6, RSC3 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11336698</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:16204215</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/RSC2_YEAST RSC2_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in meiotic sporulation through regulating IME2 expression, and is also essential for 2-micron plasmid maintenance and for normal REP1 protein localization.<ref>PMID:12024034</ref> <ref>PMID:8980231</ref> <ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12702296</ref> <ref>PMID:12183366</ref> <ref>PMID:12072455</ref> <ref>PMID:12697820</ref> [[http://www.uniprot.org/uniprot/RSC8_YEAST RSC8_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9121424</ref> [[http://www.uniprot.org/uniprot/HTL1_YEAST HTL1_YEAST]] Required for cell cycle progression through G2/M transition at temperatures higher than 33 degrees Celsius. Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. When associated with the RSC complex, may act coordinately with PKC1 to regulate G2/M transition. Together with LDB7, NPL6, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:12417720</ref> <ref>PMID:12684875</ref> <ref>PMID:16204215</ref> [[http://www.uniprot.org/uniprot/RSC7_YEAST RSC7_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization.<ref>PMID:16204215</ref> [[http://www.uniprot.org/uniprot/RSC6_YEAST RSC6_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and suppresses formamide sensitivity of the RSC8 mutants.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9685490</ref> [[http://www.uniprot.org/uniprot/RSC3_YEAST RSC3_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall, and also for proper metaphase progression. Together with HTL1, LDB7, NPL6, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11336698</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:16204215</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/SFH1_YEAST SFH1_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and required for cell cycle progression.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9154831</ref>	+	[[http://www.uniprot.org/uniprot/RSC58_YEAST RSC58_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/RSC4_YEAST RSC4_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton.<ref>PMID:8980231</ref> <ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12183366</ref> <ref>PMID:12072455</ref> <ref>PMID:12697820</ref> [[http://www.uniprot.org/uniprot/RSC9_YEAST RSC9_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit plays a role in transcriptional response to stress. It is involved in both repression and activation of mRNAs regulated by the target of rapamycin (TOR) kinases, and in the synthesis of rRNA.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11931764</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/STH1_YEAST STH1_YEAST]] Catalytic component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is the essential ATPase of the complex. It is a DNA translocase capable of nucleosome remodeling. Required for full expression of early meiotic genes. Essential for mitotic growth and repression of CHA1 expression. Also involved in G2 phase control.<ref>PMID:10025404</ref> <ref>PMID:10320476</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9799253</ref> [[http://www.uniprot.org/uniprot/RSC30_YEAST RSC30_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall. Together with HTL1, LDB7, NPL6, RSC3 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11336698</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:16204215</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/RSC8_YEAST RSC8_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9121424</ref> [[http://www.uniprot.org/uniprot/RSC2_YEAST RSC2_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in meiotic sporulation through regulating IME2 expression, and is also essential for 2-micron plasmid maintenance and for normal REP1 protein localization.<ref>PMID:12024034</ref> <ref>PMID:8980231</ref> <ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12702296</ref> <ref>PMID:12183366</ref> <ref>PMID:12072455</ref> <ref>PMID:12697820</ref> [[http://www.uniprot.org/uniprot/HTL1_YEAST HTL1_YEAST]] Required for cell cycle progression through G2/M transition at temperatures higher than 33 degrees Celsius. Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. When associated with the RSC complex, may act coordinately with PKC1 to regulate G2/M transition. Together with LDB7, NPL6, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:12417720</ref> <ref>PMID:12684875</ref> <ref>PMID:16204215</ref> [[http://www.uniprot.org/uniprot/RSC7_YEAST RSC7_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization.<ref>PMID:16204215</ref> [[http://www.uniprot.org/uniprot/RSC6_YEAST RSC6_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and suppresses formamide sensitivity of the RSC8 mutants.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9685490</ref> [[http://www.uniprot.org/uniprot/RSC3_YEAST RSC3_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall, and also for proper metaphase progression. Together with HTL1, LDB7, NPL6, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11336698</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:16204215</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/SFH1_YEAST SFH1_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and required for cell cycle progression.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9154831</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: DNA helicase]]		[[Category: DNA helicase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 11:00, 13 November 2019

OCA — Wed, 13 Nov 2019 11:00:38 GMT

←Older revision		Revision as of 11:00, 13 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6k15 is ~~ON HOLD until Paper~~ Publication	+	==RSC substrate-recruitment module==
		+	<StructureSection load='6k15' size='340' side='right'caption='[[6k15]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6k15]] is a 13 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K15 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_helicase DNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.12 3.6.4.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k15 OCA], [http://pdbe.org/6k15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k15 RCSB], [http://www.ebi.ac.uk/pdbsum/6k15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k15 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RSC58_YEAST RSC58_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/RSC4_YEAST RSC4_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton.<ref>PMID:8980231</ref> <ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12183366</ref> <ref>PMID:12072455</ref> <ref>PMID:12697820</ref> [[http://www.uniprot.org/uniprot/RSC9_YEAST RSC9_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit plays a role in transcriptional response to stress. It is involved in both repression and activation of mRNAs regulated by the target of rapamycin (TOR) kinases, and in the synthesis of rRNA.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11931764</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/STH1_YEAST STH1_YEAST]] Catalytic component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is the essential ATPase of the complex. It is a DNA translocase capable of nucleosome remodeling. Required for full expression of early meiotic genes. Essential for mitotic growth and repression of CHA1 expression. Also involved in G2 phase control.<ref>PMID:10025404</ref> <ref>PMID:10320476</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9799253</ref> [[http://www.uniprot.org/uniprot/RSC30_YEAST RSC30_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall. Together with HTL1, LDB7, NPL6, RSC3 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11336698</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:16204215</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/RSC2_YEAST RSC2_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in meiotic sporulation through regulating IME2 expression, and is also essential for 2-micron plasmid maintenance and for normal REP1 protein localization.<ref>PMID:12024034</ref> <ref>PMID:8980231</ref> <ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12702296</ref> <ref>PMID:12183366</ref> <ref>PMID:12072455</ref> <ref>PMID:12697820</ref> [[http://www.uniprot.org/uniprot/RSC8_YEAST RSC8_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and for repression of CHA1 expression.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9121424</ref> [[http://www.uniprot.org/uniprot/HTL1_YEAST HTL1_YEAST]] Required for cell cycle progression through G2/M transition at temperatures higher than 33 degrees Celsius. Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. When associated with the RSC complex, may act coordinately with PKC1 to regulate G2/M transition. Together with LDB7, NPL6, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:12417720</ref> <ref>PMID:12684875</ref> <ref>PMID:16204215</ref> [[http://www.uniprot.org/uniprot/RSC7_YEAST RSC7_YEAST]] Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Together with HTL1, LDB7, RSC3, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity. Acidic protein important for nuclear protein localization.<ref>PMID:16204215</ref> [[http://www.uniprot.org/uniprot/RSC6_YEAST RSC6_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and suppresses formamide sensitivity of the RSC8 mutants.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9685490</ref> [[http://www.uniprot.org/uniprot/RSC3_YEAST RSC3_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is required for transcription of ribosomal protein genes and genes involved in the integrity of the cell wall, and also for proper metaphase progression. Together with HTL1, LDB7, NPL6, RSC30 components, defines a fungal-specific module within the RSC complex that plays a role in many cellular functions including the maintenance of cell wall integrity.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:11336698</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:16204215</ref> <ref>PMID:8980231</ref> [[http://www.uniprot.org/uniprot/SFH1_YEAST SFH1_YEAST]] Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is essential for mitotic growth and required for cell cycle progression.<ref>PMID:10025404</ref> <ref>PMID:10329629</ref> <ref>PMID:12072455</ref> <ref>PMID:12183366</ref> <ref>PMID:12697820</ref> <ref>PMID:8980231</ref> <ref>PMID:9154831</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The RSC complex remodels chromatin structure and regulates gene transcription. We report the cryoEM structure of yeast RSC bound to the nucleosome. RSC is delineated into the ATPase motor, the actin-related-protein (ARP) module, and the substrate-recruitment module (SRM). RSC binds the nucleosome mainly through the motor, with the auxiliary subunit Sfh1 engaging the H2A-H2B acidic patch to enable nucleosome ejection. SRM is organized into three substrate-binding lobes poised to bind their respective nucleosomal epitopes. The relative orientations of the SRM and the motor on the nucleosome explain the directionality of DNA translocation and promoter nucleosome repositioning by RSC. Together, our findings shed light on RSC assembly and functionality, and provide a framework to understand the mammalian homologs BAF/PBAF and the Sfh1 ortholog INI1/BAF47, which are frequently mutated in cancers.

-	~~Authors~~:	+	Structure of the RSC complex bound to the nucleosome.,Ye Y, Wu H, Chen K, Clapier CR, Verma N, Zhang W, Deng H, Cairns BR, Gao N, Chen Z Science. 2019 Oct 31. pii: science.aay0033. doi: 10.1126/science.aay0033. PMID:31672915<ref>PMID:31672915</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6k15" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DNA helicase]]
		+	[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae s288c]]
		+	[[Category: Cairns, B]]
		+	[[Category: Chen, K J]]
		+	[[Category: Chen, Z C]]
		+	[[Category: Gao, N]]
		+	[[Category: Verma, N]]
		+	[[Category: Wu, H]]
		+	[[Category: Ye, Y P]]
		+	[[Category: Chromatin remodeler]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Swi/snf family]]

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OCA: New page: '''Unreleased structure''' The entry 6k15 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Tue, 21 May 2019 05:41:10 GMT

New page: '''Unreleased structure''' The entry 6k15 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6k15 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]