6k2z - Revision history

OCA at 10:40, 27 March 2024

OCA — Wed, 27 Mar 2024 10:40:59 GMT

←Older revision		Revision as of 10:40, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=~~GLC~~:~~ALPHA~~-D-~~GLUCOSE~~'>~~GLC~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA~~-D-~~GALACTOSE~~'>~~GAL~~</scene><~~/td~~></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></~~td><td class="sblockDat"~~>~~LGALS14, PPL13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene>, <scene name='pdbligand=PRD_900008:alpha-lactose'>PRD_900008</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [https://pdbe.org/6k2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [https://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [https://pdbe.org/6k2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [https://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PPL13_HUMAN PPL13_HUMAN]] Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>	+	[https://www.uniprot.org/uniprot/PPL13_HUMAN PPL13_HUMAN] Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The expression of prototype galectin-14 (Gal-14) in human placenta is higher than any other galectin, suggesting that it may play a role in fetal development and regulation of immune tolerance during pregnancy. Here, we solved the crystal structure of dimeric Gal-14, and found that its global fold is significantly different from that of other galectins with two beta-strands (S5 and S6) extending from one monomer and contributing to the carbohydrate binding domain of the other. The hemagglutination assay showed that this lectin could induce agglutination of chicken erythrocytes, even though lactose could not inhibit Gal-14-induced agglutination activity. Calorimetry indicates that lactose does not interact with this lectin. Compared to galectin-1, -3 and -8, Gal-14 has two key amino acids (a histidine and an arginine) in the normally conserved, canonical sugar binding site are substituted by glutamine (Gln53) and histidine (His57), thus likely explaining why lactose binding to this lectin is very weak. Lactose was observed in the ligand binding site of one Gal-14 structure, most likely because ligand binding is weak and crystals were allowed to grow over a long period of time in the presence of lactose. We also found that EGFP-tagged Gal-14 is primarily localized within the nucleus of different cell types. In addition, Gal-14 co-localized with c-Rel (a member of NF-kappaB family) in HeLa cells. These findings indicate that Gal-14 might regulate signal transduction pathways through NF-kappaB hubs. Overall, the present study provides impetus for further research into the function of Gal-14 in embryology.	+
-		+
-	Structure-function studies of galectin-14, an important effector molecule in embryology.,Si Y, Li Y, Yang T, Li X, Ayala GJ, Mayo KH, Tai G, Su J, Zhou Y FEBS J. 2020 Jun 11. doi: 10.1111/febs.15441. PMID:32525264<ref>PMID:32525264</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6k2z" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 26:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Su, J]]	+	[[Category: Su J]]
-	~~[[Category: Human galectin-14 with lactose]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 17:43, 10 March 2021

OCA — Wed, 10 Mar 2021 17:43:43 GMT

←Older revision		Revision as of 17:43, 10 March 2021
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	<StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k2z]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=~~GAL~~:~~BETA~~-D-~~GALACTOSE~~'>~~GAL~~</scene>, <scene name='pdbligand=~~GLC~~:~~ALPHA~~-D-~~GLUCOSE~~'>~~GLC~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGALS14, PPL13 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGALS14, PPL13 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [~~http~~://pdbe.org/6k2z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [https://pdbe.org/6k2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [https://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PPL13_HUMAN PPL13_HUMAN]] Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>	+	[[https://www.uniprot.org/uniprot/PPL13_HUMAN PPL13_HUMAN]] Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6k2z" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6k2z" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galectin 3D structures\|Galectin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:48, 25 June 2020

OCA — Thu, 25 Jun 2020 06:48:47 GMT

←Older revision		Revision as of 06:48, 25 June 2020
Line 1:		Line 1:

	==Human Galectin-14 with lactose==		==Human Galectin-14 with lactose==
-	<StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]]' scene=''>	+	<StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k2z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [http://pdbe.org/6k2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [http://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGALS14, PPL13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [http://pdbe.org/6k2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [http://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PPL13_HUMAN PPL13_HUMAN]] Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The expression of prototype galectin-14 (Gal-14) in human placenta is higher than any other galectin, suggesting that it may play a role in fetal development and regulation of immune tolerance during pregnancy. Here, we solved the crystal structure of dimeric Gal-14, and found that its global fold is significantly different from that of other galectins with two beta-strands (S5 and S6) extending from one monomer and contributing to the carbohydrate binding domain of the other. The hemagglutination assay showed that this lectin could induce agglutination of chicken erythrocytes, even though lactose could not inhibit Gal-14-induced agglutination activity. Calorimetry indicates that lactose does not interact with this lectin. Compared to galectin-1, -3 and -8, Gal-14 has two key amino acids (a histidine and an arginine) in the normally conserved, canonical sugar binding site are substituted by glutamine (Gln53) and histidine (His57), thus likely explaining why lactose binding to this lectin is very weak. Lactose was observed in the ligand binding site of one Gal-14 structure, most likely because ligand binding is weak and crystals were allowed to grow over a long period of time in the presence of lactose. We also found that EGFP-tagged Gal-14 is primarily localized within the nucleus of different cell types. In addition, Gal-14 co-localized with c-Rel (a member of NF-kappaB family) in HeLa cells. These findings indicate that Gal-14 might regulate signal transduction pathways through NF-kappaB hubs. Overall, the present study provides impetus for further research into the function of Gal-14 in embryology.
		+
		+	Structure-function studies of galectin-14, an important effector molecule in embryology.,Si Y, Li Y, Yang T, Li X, Ayala GJ, Mayo KH, Tai G, Su J, Zhou Y FEBS J. 2020 Jun 11. doi: 10.1111/febs.15441. PMID:32525264<ref>PMID:32525264</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6k2z" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Su J]]	+	[[Category: Su, J]]
		+	[[Category: Human galectin-14 with lactose]]
		+	[[Category: Sugar binding protein]]

OCA at 10:06, 17 June 2020

OCA — Wed, 17 Jun 2020 10:06:29 GMT

←Older revision		Revision as of 10:06, 17 June 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6k2z ~~is ON HOLD until Aug 15 2021~~	+	==Human Galectin-14 with lactose==
-		+	<StructureSection load='6k2z' size='340' side='right'caption='[[6k2z]]' scene=''>
-	~~Authors~~: ~~Su, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K2Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K2Z FirstGlance]. <br>
-	~~Description~~: ~~Human Galectin~~-~~14 with lactose~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k2z OCA], [http://pdbe.org/6k2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k2z RCSB], [http://www.ebi.ac.uk/pdbsum/6k2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k2z ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Su, J]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Su J]]

OCA at 04:07, 20 May 2020

OCA — Wed, 20 May 2020 04:07:32 GMT

←Older revision		Revision as of 04:07, 20 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k2z is ON HOLD until ~~Paper Publication~~	+	The entry 6k2z is ON HOLD until Aug 15 2021

	Authors: Su, J.		Authors: Su, J.

OCA at 06:30, 2 October 2019

OCA — Wed, 02 Oct 2019 06:30:09 GMT

←Older revision		Revision as of 06:30, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k2z is ON HOLD	+	The entry 6k2z is ON HOLD until Paper Publication

	Authors: Su, J.		Authors: Su, J.

OCA: Protected "6k2z" [edit=sysop:move=sysop]

OCA — Wed, 22 May 2019 03:56:51 GMT

Protected "6k2z" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:56, 22 May 2019

OCA: New page: '''Unreleased structure''' The entry 6k2z is ON HOLD Authors: Su, J. Description: Human Galectin-14 with lactose Category: Unreleased Structures Category: Su, J

OCA — Wed, 22 May 2019 03:56:50 GMT

New page: '''Unreleased structure''' The entry 6k2z is ON HOLD Authors: Su, J. Description: Human Galectin-14 with lactose Category: Unreleased Structures Category: Su, J

New page

'''Unreleased structure'''

The entry 6k2z is ON HOLD

Authors: Su, J.

Description: Human Galectin-14 with lactose
[[Category: Unreleased Structures]]
[[Category: Su, J]]