6k68 - Revision history

OCA at 10:27, 22 November 2023

OCA — Wed, 22 Nov 2023 10:27:14 GMT

←Older revision		Revision as of 10:27, 22 November 2023
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	<StructureSection load='6k68' size='340' side='right'caption='[[6k68]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='6k68' size='340' side='right'caption='[[6k68]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k68]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885~~] and [~~http~~://en.wikipedia.org/wiki/~~9hiv1 9hiv1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K68 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6K68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k68]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K68 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6k68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k68 OCA], [~~http~~://pdbe.org/6k68 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6k68 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6k68 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6k68 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k68 OCA], [https://pdbe.org/6k68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k68 RCSB], [https://www.ebi.ac.uk/pdbsum/6k68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k68 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SPA_STAA8 SPA_STAA8]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Cho, G Y~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Jin, M S~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Kim, J W~~]]	+	[[Category: Cho GY]]
-	[[Category: ~~Kim, S~~]]	+	[[Category: Jin MS]]
-	[[Category: ~~Lee, H~~]]	+	[[Category: Kim JW]]
-	[[Category: ~~Lee, J O~~]]	+	[[Category: Kim S]]
-	[[Category: ~~Antibody~~]]	+	[[Category: Lee H]]
-	[[Category: ~~Protein design]]~~	+	[[Category: Lee JO]]
-	~~[[Category: Structural protein~~]]	+

OCA at 17:30, 20 November 2019

OCA — Wed, 20 Nov 2019 17:30:22 GMT

←Older revision		Revision as of 17:30, 20 November 2019
Line 3:		Line 3:
	<StructureSection load='6k68' size='340' side='right'caption='[[6k68]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='6k68' size='340' side='right'caption='[[6k68]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k68]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k68]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885] and [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K68 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k68 OCA], [http://pdbe.org/6k68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k68 RCSB], [http://www.ebi.ac.uk/pdbsum/6k68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k68 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k68 OCA], [http://pdbe.org/6k68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k68 RCSB], [http://www.ebi.ac.uk/pdbsum/6k68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k68 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 16:35, 14 August 2019

OCA — Wed, 14 Aug 2019 16:35:18 GMT

←Older revision		Revision as of 16:35, 14 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6k68 is ~~ON HOLD~~	+	==Application of anti-helix antibodies in protein structure determination (8420-3MNZ)==
		+	<StructureSection load='6k68' size='340' side='right'caption='[[6k68]], [[Resolution\|resolution]] 3.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6k68]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K68 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k68 OCA], [http://pdbe.org/6k68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k68 RCSB], [http://www.ebi.ac.uk/pdbsum/6k68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k68 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antibodies are indispensable tools in protein engineering and structural biology. Antibodies suitable for structural studies should recognize the 3-dimensional (3D) conformations of target proteins. Generating such antibodies and characterizing their complexes with antigens take a significant amount of time and effort. Here, we show that we can expand the application of well-characterized antibodies by "transplanting" the epitopes that they recognize to proteins with completely different structures and sequences. Previously, several antibodies have been shown to recognize the alpha-helical conformation of antigenic peptides. We demonstrate that these antibodies can be made to bind to a variety of unrelated "off-target" proteins by modifying amino acids in the preexisting alpha helices of such proteins. Using X-ray crystallography, we determined the structures of the engineered protein-antibody complexes. All of the antibodies bound to the epitope-transplanted proteins, forming accurately predictable structures. Furthermore, we showed that binding of these antihelix antibodies to the engineered target proteins can modulate their catalytic activities by trapping them in selected functional states. Our method is simple and efficient, and it will have applications in protein X-ray crystallography, electron microscopy, and nanotechnology.

-	~~Authors: Lee, J.O~~., ~~Jin~~, M.S., ~~Kim~~, ~~J.W.~~, Kim~~, S.~~, Lee, H., ~~Cho~~, G.Y.	+	Application of antihelix antibodies in protein structure determination.,Kim JW, Kim S, Lee H, Cho G, Kim SC, Lee H, Jin MS, Lee JO Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1910080116. doi:, 10.1073/pnas.1910080116. PMID:31371498<ref>PMID:31371498</ref>

-	~~Description: Application~~ of ~~anti~~-~~helix antibodies in protein structure determination (8420~~-~~3MNZ)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6k68" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cho, G Y]]
		+	[[Category: Jin, M S]]
		+	[[Category: Kim, J W]]
	[[Category: Kim, S]]		[[Category: Kim, S]]
-	[[Category: Lee, J.O]]
	[[Category: Lee, H]]		[[Category: Lee, H]]
-	[[Category: ~~Kim~~, J.W]]	+	[[Category: Lee, J O]]
-	[[Category: ~~Jin, M.S~~]]	+	[[Category: Antibody]]
-	[[Category: ~~Cho, G.Y~~]]	+	[[Category: Protein design]]
		+	[[Category: Structural protein]]

OCA: Protected "6k68" [edit=sysop:move=sysop]

OCA — Wed, 12 Jun 2019 03:53:34 GMT

Protected "6k68" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:53, 12 June 2019

OCA: New page: '''Unreleased structure''' The entry 6k68 is ON HOLD Authors: Lee, J.O., Jin, M.S., Kim, J.W., Kim, S., Lee, H., Cho, G.Y. Description: Application of anti-helix antibodies in protein ...

OCA — Wed, 12 Jun 2019 03:53:33 GMT

New page: '''Unreleased structure''' The entry 6k68 is ON HOLD Authors: Lee, J.O., Jin, M.S., Kim, J.W., Kim, S., Lee, H., Cho, G.Y. Description: Application of anti-helix antibodies in protein ...

New page

'''Unreleased structure'''

The entry 6k68 is ON HOLD

Authors: Lee, J.O., Jin, M.S., Kim, J.W., Kim, S., Lee, H., Cho, G.Y.

Description: Application of anti-helix antibodies in protein structure determination (8420-3MNZ)
[[Category: Unreleased Structures]]
[[Category: Kim, S]]
[[Category: Lee, J.O]]
[[Category: Lee, H]]
[[Category: Kim, J.W]]
[[Category: Jin, M.S]]
[[Category: Cho, G.Y]]