6k69 - Revision history

OCA at 10:27, 22 November 2023

OCA — Wed, 22 Nov 2023 10:27:15 GMT

←Older revision		Revision as of 10:27, 22 November 2023
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	<StructureSection load='6k69' size='340' side='right'caption='[[6k69]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6k69' size='340' side='right'caption='[[6k69]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k69]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K69 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6K69 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k69]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K69 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6k69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k69 OCA], [~~http~~://pdbe.org/6k69 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6k69 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6k69 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6k69 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.401Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k69 OCA], [https://pdbe.org/6k69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k69 RCSB], [https://www.ebi.ac.uk/pdbsum/6k69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k69 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6k69" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6k69" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia virus T4]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cho~~, G Y~~]]	+	[[Category: Cho GY]]
-	[[Category: Jin~~, M S~~]]	+	[[Category: Jin MS]]
-	[[Category: Kim~~, J W~~]]	+	[[Category: Kim JW]]
-	[[Category: Kim, S]]	+	[[Category: Kim S]]
-	[[Category: Lee, H]]	+	[[Category: Lee H]]
-	[[Category: Lee~~, J O]]~~	+	[[Category: Lee JO]]
-	~~[[Category: Antibody]]~~	+
-	~~[[Category: Protein design]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 16:35, 14 August 2019

OCA — Wed, 14 Aug 2019 16:35:15 GMT

←Older revision		Revision as of 16:35, 14 August 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6k69 is ~~ON HOLD until Paper~~ Publication	+	==Application of anti-helix antibodies in protein structure determination (9213-3LRH)==
		+	<StructureSection load='6k69' size='340' side='right'caption='[[6k69]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6k69]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K69 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K69 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k69 OCA], [http://pdbe.org/6k69 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k69 RCSB], [http://www.ebi.ac.uk/pdbsum/6k69 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k69 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antibodies are indispensable tools in protein engineering and structural biology. Antibodies suitable for structural studies should recognize the 3-dimensional (3D) conformations of target proteins. Generating such antibodies and characterizing their complexes with antigens take a significant amount of time and effort. Here, we show that we can expand the application of well-characterized antibodies by "transplanting" the epitopes that they recognize to proteins with completely different structures and sequences. Previously, several antibodies have been shown to recognize the alpha-helical conformation of antigenic peptides. We demonstrate that these antibodies can be made to bind to a variety of unrelated "off-target" proteins by modifying amino acids in the preexisting alpha helices of such proteins. Using X-ray crystallography, we determined the structures of the engineered protein-antibody complexes. All of the antibodies bound to the epitope-transplanted proteins, forming accurately predictable structures. Furthermore, we showed that binding of these antihelix antibodies to the engineered target proteins can modulate their catalytic activities by trapping them in selected functional states. Our method is simple and efficient, and it will have applications in protein X-ray crystallography, electron microscopy, and nanotechnology.

-	~~Authors: Lee, J.O~~., ~~Jin~~, M.S., ~~Kim~~, ~~J.W.~~, Kim~~, S.~~, Lee, H., ~~Cho~~, G.Y.	+	Application of antihelix antibodies in protein structure determination.,Kim JW, Kim S, Lee H, Cho G, Kim SC, Lee H, Jin MS, Lee JO Proc Natl Acad Sci U S A. 2019 Aug 1. pii: 1910080116. doi:, 10.1073/pnas.1910080116. PMID:31371498<ref>PMID:31371498</ref>

-	~~Description: Application~~ of ~~anti~~-~~helix antibodies in protein structure determination (9213~~-~~3LRH)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6k69" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cho, G Y]]
		+	[[Category: Jin, M S]]
		+	[[Category: Kim, J W]]
	[[Category: Kim, S]]		[[Category: Kim, S]]
-	[[Category: Lee, J.O]]
	[[Category: Lee, H]]		[[Category: Lee, H]]
-	[[Category: ~~Kim~~, J.W]]	+	[[Category: Lee, J O]]
-	[[Category: ~~Jin, M.S~~]]	+	[[Category: Antibody]]
-	[[Category: ~~Cho, G.Y~~]]	+	[[Category: Protein design]]
		+	[[Category: Structural protein]]

OCA at 04:02, 3 July 2019

OCA — Wed, 03 Jul 2019 04:02:05 GMT

←Older revision		Revision as of 04:02, 3 July 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k69 is ON HOLD	+	The entry 6k69 is ON HOLD until Paper Publication

	Authors: Lee, J.O., Jin, M.S., Kim, J.W., Kim, S., Lee, H., Cho, G.Y.		Authors: Lee, J.O., Jin, M.S., Kim, J.W., Kim, S., Lee, H., Cho, G.Y.

OCA: Protected "6k69" [edit=sysop:move=sysop]

OCA — Wed, 12 Jun 2019 03:53:37 GMT

Protected "6k69" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:53, 12 June 2019

OCA: New page: '''Unreleased structure''' The entry 6k69 is ON HOLD Authors: Lee, J.O., Jin, M.S., Kim, J.W., Kim, S., Lee, H., Cho, G.Y. Description: Application of anti-helix antibodies in protein ...

OCA — Wed, 12 Jun 2019 03:53:36 GMT

New page: '''Unreleased structure''' The entry 6k69 is ON HOLD Authors: Lee, J.O., Jin, M.S., Kim, J.W., Kim, S., Lee, H., Cho, G.Y. Description: Application of anti-helix antibodies in protein ...

New page

'''Unreleased structure'''

The entry 6k69 is ON HOLD

Authors: Lee, J.O., Jin, M.S., Kim, J.W., Kim, S., Lee, H., Cho, G.Y.

Description: Application of anti-helix antibodies in protein structure determination (9213-3LRH)
[[Category: Unreleased Structures]]
[[Category: Kim, S]]
[[Category: Lee, J.O]]
[[Category: Lee, H]]
[[Category: Kim, J.W]]
[[Category: Jin, M.S]]
[[Category: Cho, G.Y]]