6k73 - Revision history

OCA at 06:06, 14 October 2020

OCA — Wed, 14 Oct 2020 06:06:21 GMT

←Older revision		Revision as of 06:06, 14 October 2020
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	==Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis==		==Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis==
-	<StructureSection load='6k73' size='340' side='right'caption='[[6k73]]' scene=''>	+	<StructureSection load='6k73' size='340' side='right'caption='[[6k73]], [[Resolution\|resolution]] 2.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K73 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K73 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k73]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K73 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K73 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k73 OCA], [http://pdbe.org/6k73 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k73 RCSB], [http://www.ebi.ac.uk/pdbsum/6k73 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k73 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D4V05_24280 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), cfaE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k73 OCA], [http://pdbe.org/6k73 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k73 RCSB], [http://www.ebi.ac.uk/pdbsum/6k73 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k73 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Colonization factor CFA/I defines the major adhesive fimbriae of enterotoxigenic Escherichia coli and mediates bacterial attachment to host intestinal epithelial cells. The CFA/I fimbria consists of a tip-localized minor adhesive subunit, CfaE, and thousands of copies of the major subunit CfaB polymerized into an ordered helical rod. Biosynthesis of CFA/I fimbriae requires the assistance of the periplasmic chaperone CfaA and outer membrane usher CfaC. Although the CfaE subunit is proposed to initiate the assembly of CFA/I fimbriae, how it performs this function remains elusive. Here, we report the establishment of an in vitro assay for CFA/I fimbria assembly and show that stabilized CfaA-CfaB and CfaA-CfaE binary complexes together with CfaC are sufficient to drive fimbria formation. The presence of both CfaA-CfaE and CfaC accelerates fimbria formation, while the absence of either component leads to linearized CfaB polymers in vitro. We further report the crystal structure of the stabilized CfaA-CfaE complex, revealing features unique for biogenesis of Class 5 fimbriae.
		+
		+	Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis.,He LH, Wang H, Liu Y, Kang M, Li T, Li CC, Tong AP, Zhu YB, Song YJ, Savarino SJ, Prouty MG, Xia D, Bao R PLoS Pathog. 2020 Oct 2;16(10):e1008848. doi: 10.1371/journal.ppat.1008848., eCollection 2020 Oct. PMID:33007034<ref>PMID:33007034</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6k73" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bao R]]	+	[[Category: Bao, R]]
-	[[Category: He LH]]	+	[[Category: He, L H]]
		+	[[Category: Adhesive subunit]]
		+	[[Category: Binding motif]]
		+	[[Category: Chaperone]]
		+	[[Category: Chaperone-usher fimbriae]]
		+	[[Category: Enterotoxigenic escherichia coli]]
		+	[[Category: Periplasmic chaperone]]

OCA at 06:22, 10 June 2020

OCA — Wed, 10 Jun 2020 06:22:18 GMT

←Older revision		Revision as of 06:22, 10 June 2020
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-	'''Unreleased structure'''

-	~~The entry 6k73 is ON HOLD until Jun 05 2021~~	+	==Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis==
-		+	<StructureSection load='6k73' size='340' side='right'caption='[[6k73]]' scene=''>
-	~~Authors: Bao, R., He, L.H.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K73 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K73 FirstGlance]. <br>
-	~~Description:~~ Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k73 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k73 OCA], [http://pdbe.org/6k73 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k73 RCSB], [http://www.ebi.ac.uk/pdbsum/6k73 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k73 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: He, L.H]]	+	__TOC__
-	[[Category: Bao, R]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bao R]]
		+	[[Category: He LH]]

OCA at 06:30, 2 October 2019

OCA — Wed, 02 Oct 2019 06:30:30 GMT

←Older revision		Revision as of 06:30, 2 October 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k73 is ON HOLD	+	The entry 6k73 is ON HOLD until Jun 05 2021

	Authors: Bao, R., He, L.H.		Authors: Bao, R., He, L.H.

OCA: Protected "6k73" [edit=sysop:move=sysop]

OCA — Wed, 12 Jun 2019 03:55:58 GMT

Protected "6k73" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:55, 12 June 2019

OCA: New page: '''Unreleased structure''' The entry 6k73 is ON HOLD Authors: Bao, R., He, L.H. Description: Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogene...

OCA — Wed, 12 Jun 2019 03:55:56 GMT

New page: '''Unreleased structure''' The entry 6k73 is ON HOLD Authors: Bao, R., He, L.H. Description: Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogene...

New page

'''Unreleased structure'''

The entry 6k73 is ON HOLD

Authors: Bao, R., He, L.H.

Description: Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis
[[Category: Unreleased Structures]]
[[Category: He, L.H]]
[[Category: Bao, R]]