6k7w - Revision history

OCA at 10:53, 14 June 2023

OCA — Wed, 14 Jun 2023 10:53:10 GMT

←Older revision		Revision as of 10:53, 14 June 2023
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	==Solution Structure of the CS1 Domain of USP19==		==Solution Structure of the CS1 Domain of USP19==
-	<StructureSection load='6k7w' size='340' side='right'caption='[[6k7w~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='6k7w' size='340' side='right'caption='[[6k7w]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k7w]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6K7W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k7w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K7W FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">USP19, KIAA0891, ZMYND9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7w OCA], [https://pdbe.org/6k7w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k7w RCSB], [https://www.ebi.ac.uk/pdbsum/6k7w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7w ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitinyl_hydrolase_1 Ubiquitinyl hydrolase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.12 3.4.19.12] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6k7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7w OCA], [~~http~~://pdbe.org/6k7w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6k7w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6k7w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7w ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UBP19_HUMAN UBP19_HUMAN]] Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing their ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains.<ref>PMID:19465887</ref> <ref>PMID:21849505</ref> <ref>PMID:22128162</ref> <ref>PMID:22689415</ref>	+	[https://www.uniprot.org/uniprot/UBP19_HUMAN UBP19_HUMAN] Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing their ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains.<ref>PMID:19465887</ref> <ref>PMID:21849505</ref> <ref>PMID:22128162</ref> <ref>PMID:22689415</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Ubiquitinyl hydrolase 1]]~~	+	[[Category: Hu HY]]
-	[[Category: Hu~~, H Y~~]]	+	[[Category: Xue W]]
-	[[Category: Xue, W]]	+
-	~~[[Category: Autoinhibitory regulation]]~~	+
-	~~[[Category: Cs domain]]~~	+
-	~~[[Category: Hydrolase inhibitor~~]]	+

OCA at 10:47, 24 December 2020

OCA — Thu, 24 Dec 2020 10:47:22 GMT

←Older revision		Revision as of 10:47, 24 December 2020
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	==Solution Structure of the CS1 Domain of USP19==		==Solution Structure of the CS1 Domain of USP19==
-	<StructureSection load='6k7w' size='340' side='right'caption='[[6k7w]]' scene=''>	+	<StructureSection load='6k7w' size='340' side='right'caption='[[6k7w]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K7W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k7w]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K7W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7w OCA], [http://pdbe.org/6k7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7w RCSB], [http://www.ebi.ac.uk/pdbsum/6k7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7w ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">USP19, KIAA0891, ZMYND9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitinyl_hydrolase_1 Ubiquitinyl hydrolase 1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.19.12 3.4.19.12] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7w OCA], [http://pdbe.org/6k7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7w RCSB], [http://www.ebi.ac.uk/pdbsum/6k7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UBP19_HUMAN UBP19_HUMAN]] Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates. Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing their ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains.<ref>PMID:19465887</ref> <ref>PMID:21849505</ref> <ref>PMID:22128162</ref> <ref>PMID:22689415</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ubiquitin-specific protease 19 (USP19) is a member of the deubiquitinating (DUB) enzymes that catalyze removing the ubiquitin signals from target proteins. Our previous research has demonstrated that USP19 up-regulates the protein level and aggregation of polyQ-expanded huntingtin through the involvement of heat shock protein 90 (HSP90). Here, we present solution structures of the CS1, CS2 and UbL domains of USP19 and structural insights into their domain interactions. We found that the tandem CS domains fold back to interact with the C-terminal USP domain (USPD) intra-molecularly that leads to inhibition of the catalytic core of USP19, especially CS1 interacts with the embedded UbL domain and CS2 does with the CH2 catalytic core. Moreover, CS2 specifically interacts with the NBD domain of HSP90, which can activate the DUB enzyme. A mechanism of auto-inhibition of USP19 and activation by HSP90 is proposed, on which USP19 modulates the protein level of polyQ-expanded huntingtin in cells. This study provides structural and mechanistic insights into the modulation of protein level and aggregation by USP19 with the assistance of HSP90.
		+
		+	Domain interactions reveal auto-inhibition of the deubiquitinating enzyme USP19 and its activation by HSP90 in the modulation of huntingtin aggregation.,Xue W, Zhang SX, He WT, Hong JY, Jiang LL, Hu HY Biochem J. 2020 Nov 13;477(21):4295-4312. doi: 10.1042/BCJ20200536. PMID:33094816<ref>PMID:33094816</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6k7w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Thioesterase 3D structures\|Thioesterase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hu HY]]	+	[[Category: Ubiquitinyl hydrolase 1]]
-	[[Category: Xue W]]	+	[[Category: Hu, H Y]]
		+	[[Category: Xue, W]]
		+	[[Category: Autoinhibitory regulation]]
		+	[[Category: Cs domain]]
		+	[[Category: Hydrolase inhibitor]]

OCA at 06:22, 10 June 2020

OCA — Wed, 10 Jun 2020 06:22:35 GMT

←Older revision		Revision as of 06:22, 10 June 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6k7w ~~is ON HOLD until Jun 10 2021~~	+	==Solution Structure of the CS1 Domain of USP19==
-		+	<StructureSection load='6k7w' size='340' side='right'caption='[[6k7w]]' scene=''>
-	~~Authors~~: ~~Xue~~, W., Hu, H.Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K7W FirstGlance]. <br>
-	~~Description: Solution Structure of the CS1 Domain of USP19~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k7w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7w OCA], [http://pdbe.org/6k7w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7w RCSB], [http://www.ebi.ac.uk/pdbsum/6k7w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7w ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Xue, W~~]]	+	__TOC__
-	[[Category: ~~Hu, H.Y~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hu HY]]
		+	[[Category: Xue W]]

OCA at 04:57, 6 November 2019

OCA — Wed, 06 Nov 2019 04:57:27 GMT

←Older revision		Revision as of 04:57, 6 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k7w is ON HOLD	+	The entry 6k7w is ON HOLD until Jun 10 2021

	Authors: Xue, W., Hu, H.Y.		Authors: Xue, W., Hu, H.Y.

OCA: Protected "6k7w" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2019 04:05:05 GMT

Protected "6k7w" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 19 June 2019

OCA: New page: '''Unreleased structure''' The entry 6k7w is ON HOLD Authors: Xue, W., Hu, H.Y. Description: Solution Structure of the CS1 Domain of USP19 Category: Unreleased Structures [[Categor...

OCA — Wed, 19 Jun 2019 04:05:03 GMT

New page: '''Unreleased structure''' The entry 6k7w is ON HOLD Authors: Xue, W., Hu, H.Y. Description: Solution Structure of the CS1 Domain of USP19 Category: Unreleased Structures [[Categor...

New page

'''Unreleased structure'''

The entry 6k7w is ON HOLD

Authors: Xue, W., Hu, H.Y.

Description: Solution Structure of the CS1 Domain of USP19
[[Category: Unreleased Structures]]
[[Category: Xue, W]]
[[Category: Hu, H.Y]]