6k7z - Revision history

OCA at 10:27, 22 November 2023

OCA — Wed, 22 Nov 2023 10:27:51 GMT

←Older revision		Revision as of 10:27, 22 November 2023
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	==Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia==		==Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia==
-	<StructureSection load='6k7z' size='340' side='right'caption='[[6k7z]]' scene=''>	+	<StructureSection load='6k7z' size='340' side='right'caption='[[6k7z]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6K7Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k7z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_aurantia Pseudoalteromonas aurantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K7Z FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7z OCA], [~~http~~://pdbe.org/6k7z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6k7z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6k7z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7z ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.799Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7z OCA], [https://pdbe.org/6k7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k7z RCSB], [https://www.ebi.ac.uk/pdbsum/6k7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7z ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A650AJ72_9GAMM A0A650AJ72_9GAMM]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial endochitinases play important roles in environmental chitin degradation and have good applications. Although the structures of some endochitinases, most belonging to the glycoside hydrolase (GH) family 18 and thermostable, have been reported, the structural basis of these enzymes for chitin degradation still remain unclear due to the lack of functional confirmation, and the molecular mechanism for their thermostability is also unknown. Here, we characterized a GH18 endochitinase, Chi23, from marine bacterium Pseudoalteromonas aurantia DSM6057, and solved its structure. Chi23 is a thermostable enzyme that can non-processively hydrolyze crystalline and colloidal chitin. Chi23 contains only a catalytic domain that adopts a classical (beta/alpha)8 TIM-barrel fold. Compared to other GH18 bacterial endochitinases, Chi23 lacks the chitin-binding domain and the beta-hairpin subdomain, indicating that Chi23 has a novel structure. Based on structural analysis of Chi23 docked with (GlcNAc)5 and mutational analysis, the key catalytic residue (Glu117) and seven substrate-binding residues (Asn9, Gln157, Tyr189, Asn190, Asp229, Trp260, and Gln261) are revealed. Among these identified residues, Asn9, Asp229 and Gln261 are unique to Chi23, and their cumulative roles contribute to the activity of Chi23 against both crystalline and soluble chitin. Five substrate-binding residues (Tyr189, Asn190, Asp229, Trp260, and Gln261) are found to play important roles in maintaining the thermostability of Chi23. In particular, hydrogen bond networks involving Asp229 and Gln261 are formed to stabilize the protein structure of Chi23. Phylogenetic analysis indicated that Chi23 and its homologs represent a new group of GH18 endochitinases, which are widely distributed in bacteria. This study sheds light on the molecular mechanism of a GH18 endochitinase for chitin degradation.
		+
		+	Structural Insight Into Chitin Degradation and Thermostability of a Novel Endochitinase From the Glycoside Hydrolase Family 18.,Wang YJ, Jiang WX, Zhang YS, Cao HY, Zhang Y, Chen XL, Li CY, Wang P, Zhang YZ, Song XY, Li PY Front Microbiol. 2019 Oct 30;10:2457. doi: 10.3389/fmicb.2019.02457. eCollection , 2019. PMID:31736903<ref>PMID:31736903</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6k7z" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chitinase 3D structures\|Chitinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudoalteromonas aurantia]]
	[[Category: Cao HY]]		[[Category: Cao HY]]
	[[Category: Chen XL]]		[[Category: Chen XL]]

OCA at 06:22, 10 June 2020

OCA — Wed, 10 Jun 2020 06:22:40 GMT

←Older revision		Revision as of 06:22, 10 June 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6k7z ~~is ON HOLD until Jun 10 2021~~	+	==Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia==
-		+	<StructureSection load='6k7z' size='340' side='right'caption='[[6k7z]]' scene=''>
-	~~Authors~~: ~~Wang, Y~~.J., ~~Li, P~~.Y., ~~Cao~~, H.Y., ~~Chen, X~~.L., ~~Zhang, Y~~.Z.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K7Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K7Z FirstGlance]. <br>
-	~~Description: Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k7z OCA], [http://pdbe.org/6k7z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k7z RCSB], [http://www.ebi.ac.uk/pdbsum/6k7z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k7z ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cao~~, H.Y~~]]	+	__TOC__
-	[[Category: ~~Zhang, Y.Z~~]]	+	</StructureSection>
-	[[Category: Li~~, P.Y~~]]	+	[[Category: Large Structures]]
-	[[Category: Wang~~, Y.J~~]]	+	[[Category: Cao HY]]
-	[[Category: ~~Chen, X.L~~]]	+	[[Category: Chen XL]]
		+	[[Category: Li PY]]
		+	[[Category: Wang YJ]]
		+	[[Category: Zhang YZ]]

OCA at 04:02, 3 July 2019

OCA — Wed, 03 Jul 2019 04:02:28 GMT

←Older revision		Revision as of 04:02, 3 July 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k7z is ON HOLD	+	The entry 6k7z is ON HOLD until Jun 10 2021

	Authors: Wang, Y.J., Li, P.Y., Cao, H.Y., Chen, X.L., Zhang, Y.Z.		Authors: Wang, Y.J., Li, P.Y., Cao, H.Y., Chen, X.L., Zhang, Y.Z.

OCA: Protected "6k7z" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2019 04:05:21 GMT

Protected "6k7z" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 19 June 2019

OCA: New page: '''Unreleased structure''' The entry 6k7z is ON HOLD Authors: Wang, Y.J., Li, P.Y., Cao, H.Y., Chen, X.L., Zhang, Y.Z. Description: Crystal structure of a GH18 chitinase from Pseudoalt...

OCA — Wed, 19 Jun 2019 04:05:19 GMT

New page: '''Unreleased structure''' The entry 6k7z is ON HOLD Authors: Wang, Y.J., Li, P.Y., Cao, H.Y., Chen, X.L., Zhang, Y.Z. Description: Crystal structure of a GH18 chitinase from Pseudoalt...

New page

'''Unreleased structure'''

The entry 6k7z is ON HOLD

Authors: Wang, Y.J., Li, P.Y., Cao, H.Y., Chen, X.L., Zhang, Y.Z.

Description: Crystal structure of a GH18 chitinase from Pseudoalteromonas aurantia
[[Category: Unreleased Structures]]
[[Category: Cao, H.Y]]
[[Category: Zhang, Y.Z]]
[[Category: Li, P.Y]]
[[Category: Wang, Y.J]]
[[Category: Chen, X.L]]