6k84 - Revision history

OCA at 10:53, 14 June 2023

OCA — Wed, 14 Jun 2023 10:53:12 GMT

←Older revision		Revision as of 10:53, 14 June 2023
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	==Structure of anti-prion RNA aptamer==		==Structure of anti-prion RNA aptamer==
-	<StructureSection load='6k84' size='340' side='right'caption='[[6k84~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='6k84' size='340' side='right'caption='[[6k84]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6k84]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K84 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6K84 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6k84]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K84 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6k84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k84 OCA], [~~http~~://pdbe.org/6k84 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6k84 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6k84 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6k84 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k84 OCA], [https://pdbe.org/6k84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k84 RCSB], [https://www.ebi.ac.uk/pdbsum/6k84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k84 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hayashi, T]]	+	[[Category: Synthetic construct]]
-	[[Category: Katahira, M]]	+	[[Category: Hayashi T]]
-	[[Category: Kinoshita, M]]	+	[[Category: Katahira M]]
-	[[Category: Lee~~, J H~~]]	+	[[Category: Kinoshita M]]
-	[[Category: Mashima, T]]	+	[[Category: Lee JH]]
-	[[Category: Nagata, T]]	+	[[Category: Mashima T]]
-	~~[[Category: Aptamer]]~~	+	[[Category: Nagata T]]
-	~~[[Category: Quadruplex]]~~	+
-	~~[[Category: Rna~~]]	+

OCA at 09:06, 1 April 2020

OCA — Wed, 01 Apr 2020 09:06:36 GMT

←Older revision		Revision as of 09:06, 1 April 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6k84 is ~~ON HOLD until Jun 11 2021~~	+	==Structure of anti-prion RNA aptamer==
		+	<StructureSection load='6k84' size='340' side='right'caption='[[6k84]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6k84]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K84 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K84 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k84 OCA], [http://pdbe.org/6k84 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k84 RCSB], [http://www.ebi.ac.uk/pdbsum/6k84 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k84 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Prion diseases comprise a fatal neuropathy caused by the conversion of prion protein from a cellular (PrP(C)) to a pathological (PrP(Sc)) isoform. Previously, we obtained an RNA aptamer, r(GGAGGAGGAGGA) (R12), that folds into a unique G-quadruplex. The R12 homodimer binds to a PrP(C) molecule, inhibiting PrP(C)-to-PrP(Sc) conversion. Here, we developed a new RNA aptamer, r(GGAGGAGGAGGAGGAGGAGGAGGA) (R24), where two R12s are tandemly connected. The 50% inhibitory concentration for the formation of PrP(Sc) (IC50) of R24 in scrapie-infected cell lines was ca. 100 nM, i.e., much lower than that of R12 by two orders. Except for some antibodies, R24 exhibited the lowest recorded IC50 and the highest anti-prion activity. We also developed a related aptamer, r(GGAGGAGGAGGA-A-GGAGGAGGAGGA) (R12-A-R12), IC50 being ca. 500 nM. The structure of a single R12-A-R12 molecule determined by NMR resembled that of the R12 homodimer. The quadruplex structure of either R24 or R12-A-R12 is unimolecular, and therefore the structure could be stably formed when they are administered to a prion-infected cell culture. This may be the reason they can exert high anti-prion activity.

-	~~Authors~~:	+	Development and structural determination of an anti-PrP(C) aptamer that blocks pathological conformational conversion of prion protein.,Mashima T, Lee JH, Kamatari YO, Hayashi T, Nagata T, Nishikawa F, Nishikawa S, Kinoshita M, Kuwata K, Katahira M Sci Rep. 2020 Mar 18;10(1):4934. doi: 10.1038/s41598-020-61966-4. PMID:32188933<ref>PMID:32188933</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6k84" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hayashi, T]]
		+	[[Category: Katahira, M]]
		+	[[Category: Kinoshita, M]]
		+	[[Category: Lee, J H]]
		+	[[Category: Mashima, T]]
		+	[[Category: Nagata, T]]
		+	[[Category: Aptamer]]
		+	[[Category: Quadruplex]]
		+	[[Category: Rna]]

OCA at 04:02, 3 July 2019

OCA — Wed, 03 Jul 2019 04:02:40 GMT

←Older revision		Revision as of 04:02, 3 July 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6k84 is ON HOLD	+	The entry 6k84 is ON HOLD until Jun 11 2021

	Authors:		Authors:

OCA: Protected "6k84" [edit=sysop:move=sysop]

OCA — Wed, 19 Jun 2019 04:05:50 GMT

Protected "6k84" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 19 June 2019

OCA: New page: '''Unreleased structure''' The entry 6k84 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 Jun 2019 04:05:48 GMT

New page: '''Unreleased structure''' The entry 6k84 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6k84 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]