6kg9 - Revision history

OCA at 07:35, 1 May 2024

OCA — Wed, 01 May 2024 07:35:47 GMT

←Older revision		Revision as of 07:35, 1 May 2024
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	==Solution structure of CaDoc0917 from Clostridium acetobutylicum==		==Solution structure of CaDoc0917 from Clostridium acetobutylicum==
-	<StructureSection load='6kg9' size='340' side='right'caption='[[6kg9~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6kg9' size='340' side='right'caption='[[6kg9]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kg9]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cloab Cloab~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KG9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KG9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kg9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_acetobutylicum_ATCC_824 Clostridium acetobutylicum ATCC 824]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KG9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KG9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6kg8\|6kg8]], [[6kgf\|6kgf]], [[6kge\|6kge]], [[6kgd\|6kgd]], [[6kgc\|6kgc]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">CA_C0917 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272562 CLOAB])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kg9 OCA], [https://pdbe.org/6kg9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kg9 RCSB], [https://www.ebi.ac.uk/pdbsum/6kg9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kg9 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kg9 OCA], [~~http~~://pdbe.org/6kg9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kg9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kg9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kg9 ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q97KK2_CLOAB Q97KK2_CLOAB]
-	Many important proteins undergo pH-dependent conformational changes resulting in "on-off" switches for protein function, which are essential for regulation of life processes and have wide application potential. Here, we report a pair of cellulosomal assembly modules, comprising a cohesin and a dockerin from Clostridium acetobutylicum, which interact together following a unique pH-dependent switch between two functional sites rather than on-off states. The two cohesin-binding sites on the dockerin are switched from one to the other at pH 4.8 and 7.5 with a 180 degrees rotation of the bound dockerin. Combined analysis by nuclear magnetic resonance spectroscopy, crystal structure determination, mutagenesis, and isothermal titration calorimetry elucidates the chemical and structural mechanism of the pH-dependent switching of the binding sites. The pH-dependent dual-binding-site switch not only represents an elegant example of biological regulation but also provides a new approach for developing pH-dependent protein devices and biomaterials beyond an on-off switch for biotechnological applications.	+
-		+
-	Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules.,Yao X, Chen C, Wang Y, Dong S, Liu YJ, Li Y, Cui Z, Gong W, Perrett S, Yao L, Lamed R, Bayer EA, Cui Q, Feng Y Sci Adv. 2020 Oct 23;6(43). pii: 6/43/~~eabd7182~~. ~~doi: 10~~.~~1126~~/~~sciadv.abd7182., Print 2020 Oct. PMID:33097546<ref>PMID:33097546</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6kg9" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cloab~~]]	+	[[Category: Clostridium acetobutylicum ATCC 824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Feng, Y]]	+	[[Category: Feng Y]]
-	[[Category: Yao, X]]	+	[[Category: Yao X]]
-	~~[[Category: Calcium-binding]]~~	+
-	~~[[Category: Cellulosome]]~~	+
-	~~[[Category: Dockerin]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:30, 4 November 2020

OCA — Wed, 04 Nov 2020 07:30:51 GMT

←Older revision		Revision as of 07:30, 4 November 2020
Line 1:		Line 1:

	==Solution structure of CaDoc0917 from Clostridium acetobutylicum==		==Solution structure of CaDoc0917 from Clostridium acetobutylicum==
-	<StructureSection load='6kg9' size='340' side='right'caption='[[6kg9]]' scene=''>	+	<StructureSection load='6kg9' size='340' side='right'caption='[[6kg9]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KG9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KG9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kg9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cloab Cloab]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KG9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KG9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kg9 OCA], [http://pdbe.org/6kg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kg9 RCSB], [http://www.ebi.ac.uk/pdbsum/6kg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kg9 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6kg8\|6kg8]], [[6kgf\|6kgf]], [[6kge\|6kge]], [[6kgd\|6kgd]], [[6kgc\|6kgc]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CA_C0917 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272562 CLOAB])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kg9 OCA], [http://pdbe.org/6kg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kg9 RCSB], [http://www.ebi.ac.uk/pdbsum/6kg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kg9 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many important proteins undergo pH-dependent conformational changes resulting in "on-off" switches for protein function, which are essential for regulation of life processes and have wide application potential. Here, we report a pair of cellulosomal assembly modules, comprising a cohesin and a dockerin from Clostridium acetobutylicum, which interact together following a unique pH-dependent switch between two functional sites rather than on-off states. The two cohesin-binding sites on the dockerin are switched from one to the other at pH 4.8 and 7.5 with a 180 degrees rotation of the bound dockerin. Combined analysis by nuclear magnetic resonance spectroscopy, crystal structure determination, mutagenesis, and isothermal titration calorimetry elucidates the chemical and structural mechanism of the pH-dependent switching of the binding sites. The pH-dependent dual-binding-site switch not only represents an elegant example of biological regulation but also provides a new approach for developing pH-dependent protein devices and biomaterials beyond an on-off switch for biotechnological applications.
		+
		+	Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules.,Yao X, Chen C, Wang Y, Dong S, Liu YJ, Li Y, Cui Z, Gong W, Perrett S, Yao L, Lamed R, Bayer EA, Cui Q, Feng Y Sci Adv. 2020 Oct 23;6(43). pii: 6/43/eabd7182. doi: 10.1126/sciadv.abd7182., Print 2020 Oct. PMID:33097546<ref>PMID:33097546</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kg9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cloab]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Feng Y]]	+	[[Category: Feng, Y]]
-	[[Category: Yao X]]	+	[[Category: Yao, X]]
		+	[[Category: Calcium-binding]]
		+	[[Category: Cellulosome]]
		+	[[Category: Dockerin]]
		+	[[Category: Hydrolase]]

OCA at 13:59, 8 July 2020

OCA — Wed, 08 Jul 2020 13:59:21 GMT

←Older revision		Revision as of 13:59, 8 July 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6kg9 ~~is ON HOLD until Paper Publication~~	+	==Solution structure of CaDoc0917 from Clostridium acetobutylicum==
-		+	<StructureSection load='6kg9' size='340' side='right'caption='[[6kg9]]' scene=''>
-	~~Authors~~: ~~Feng~~, Y., ~~Yao~~, X.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KG9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KG9 FirstGlance]. <br>
-	~~Description: Solution structure of CaDoc0917 from Clostridium acetobutylicum~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kg9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kg9 OCA], [http://pdbe.org/6kg9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kg9 RCSB], [http://www.ebi.ac.uk/pdbsum/6kg9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kg9 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Yao, X~~]]	+	__TOC__
-	[[Category: ~~Feng, Y~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Feng Y]]
		+	[[Category: Yao X]]

OCA at 04:13, 24 July 2019

OCA — Wed, 24 Jul 2019 04:13:24 GMT

←Older revision		Revision as of 04:13, 24 July 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kg9 is ON HOLD	+	The entry 6kg9 is ON HOLD until Paper Publication

	Authors: Feng, Y., Yao, X.		Authors: Feng, Y., Yao, X.

OCA: Protected "6kg9" [edit=sysop:move=sysop]

OCA — Wed, 17 Jul 2019 08:46:46 GMT

Protected "6kg9" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:46, 17 July 2019

OCA: New page: '''Unreleased structure''' The entry 6kg9 is ON HOLD Authors: Feng, Y., Yao, X. Description: Solution structure of CaDoc0917 from Clostridium acetobutylicum [[Category: Unreleased Stru...

OCA — Wed, 17 Jul 2019 08:46:44 GMT

New page: '''Unreleased structure''' The entry 6kg9 is ON HOLD Authors: Feng, Y., Yao, X. Description: Solution structure of CaDoc0917 from Clostridium acetobutylicum [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 6kg9 is ON HOLD

Authors: Feng, Y., Yao, X.

Description: Solution structure of CaDoc0917 from Clostridium acetobutylicum
[[Category: Unreleased Structures]]
[[Category: Yao, X]]
[[Category: Feng, Y]]