6kj1 - Revision history

OCA at 10:43, 27 March 2024

OCA — Wed, 27 Mar 2024 10:43:02 GMT

←Older revision		Revision as of 10:43, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6kj1' size='340' side='right'caption='[[6kj1]], [[Resolution\|resolution]] 0.65Å' scene=''>		<StructureSection load='6kj1' size='340' side='right'caption='[[6kj1]], [[Resolution\|resolution]] 0.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kj1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJ1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6KJ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kj1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KJ1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6kj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kj1 OCA], [~~http~~://pdbe.org/6kj1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kj1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kj1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kj1 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution\|Resolution]] 0.65Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kj1 OCA], [https://pdbe.org/6kj1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kj1 RCSB], [https://www.ebi.ac.uk/pdbsum/6kj1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kj1 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/FUS_HUMAN FUS_HUMAN]] Frontotemporal dementia with motor neuron disease;Hereditary essential tremor;Amyotrophic lateral sclerosis;Juvenile amyotrophic lateral sclerosis;Myxofibrosarcoma;Myxoid/round cell liposarcoma. A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3. A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG. The disease may be caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.	+	[https://www.uniprot.org/uniprot/FUS_HUMAN FUS_HUMAN] Frontotemporal dementia with motor neuron disease;Hereditary essential tremor;Amyotrophic lateral sclerosis;Juvenile amyotrophic lateral sclerosis;Myxofibrosarcoma;Myxoid/round cell liposarcoma. A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3. A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG. The disease may be caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FUS_HUMAN FUS_HUMAN]] Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.	+	[https://www.uniprot.org/uniprot/FUS_HUMAN FUS_HUMAN] Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Microcrystal electron diffraction (MicroED) is becoming a powerful tool in determining the crystal structures of biological macromolecules and small organic compounds. However, wide applications of this technique are still limited by the special requirement for radiation-tolerated movie-mode camera and the lack of automated data collection methods. Herein, we develop a stage-camera synchronization scheme to minimize the hardware requirements and enable the use of the conventional electron cryo-microscope with a single-frame CCD camera, which ensures not only the acquisition of ultrahigh-resolution diffraction data but also low cost in practice. This method renders the structure determination of both peptide and small organic compounds at ultrahigh resolution up to approximately 0.60 A with unambiguous assignment of nearly all hydrogen atoms. The present work provides a widely applicable solution for routine structure determination of MicroED and demonstrates the capability of the low-end 120 kV microscope with a CCD camera in solving ultrahigh resolution structures of both organic compounds and biological macromolecules.	+
-		+
-	Programming Conventional Electron Microscopes for Solving Ultrahigh-Resolution Structures of Small and Macro-Molecules.,Zhou H, Luo F, Luo Z, Li D, Liu C, Li X Anal Chem. 2019 Sep 3;91(17):10996-11003. doi: 10.1021/acs.analchem.9b01162. Epub, 2019 Aug 15. PMID:31334636<ref>PMID:31334636</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6kj1" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Li, D]]	+	[[Category: Li D]]
-	[[Category: Li, X]]	+	[[Category: Li X]]
-	[[Category: Liu, C]]	+	[[Category: Liu C]]
-	[[Category: Luo, F]]	+	[[Category: Luo F]]
-	[[Category: Luo, Z]]	+	[[Category: Luo Z]]
-	[[Category: Zhou, H]]	+	[[Category: Zhou H]]
-	~~[[Category: Fus]]~~	+
-	~~[[Category: Microed]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Ultrahigh resolution~~]]	+

OCA at 10:24, 2 October 2019

OCA — Wed, 02 Oct 2019 10:24:29 GMT

←Older revision		Revision as of 10:24, 2 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The entry ~~6kj1~~ is ~~ON HOLD~~	+	==200kV MicroED structure of FUS (37-42) SYSGYS solved from merged datasets at 0.65 A==
		+	<StructureSection load='6kj1' size='340' side='right'caption='[[6kj1]], [[Resolution\|resolution]] 0.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6kj1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KJ1 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kj1 OCA], [http://pdbe.org/6kj1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kj1 RCSB], [http://www.ebi.ac.uk/pdbsum/6kj1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kj1 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/FUS_HUMAN FUS_HUMAN]] Frontotemporal dementia with motor neuron disease;Hereditary essential tremor;Amyotrophic lateral sclerosis;Juvenile amyotrophic lateral sclerosis;Myxofibrosarcoma;Myxoid/round cell liposarcoma. A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3. A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG. The disease may be caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FUS_HUMAN FUS_HUMAN]] Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microcrystal electron diffraction (MicroED) is becoming a powerful tool in determining the crystal structures of biological macromolecules and small organic compounds. However, wide applications of this technique are still limited by the special requirement for radiation-tolerated movie-mode camera and the lack of automated data collection methods. Herein, we develop a stage-camera synchronization scheme to minimize the hardware requirements and enable the use of the conventional electron cryo-microscope with a single-frame CCD camera, which ensures not only the acquisition of ultrahigh-resolution diffraction data but also low cost in practice. This method renders the structure determination of both peptide and small organic compounds at ultrahigh resolution up to approximately 0.60 A with unambiguous assignment of nearly all hydrogen atoms. The present work provides a widely applicable solution for routine structure determination of MicroED and demonstrates the capability of the low-end 120 kV microscope with a CCD camera in solving ultrahigh resolution structures of both organic compounds and biological macromolecules.

-	~~Authors~~:	+	Programming Conventional Electron Microscopes for Solving Ultrahigh-Resolution Structures of Small and Macro-Molecules.,Zhou H, Luo F, Luo Z, Li D, Liu C, Li X Anal Chem. 2019 Sep 3;91(17):10996-11003. doi: 10.1021/acs.analchem.9b01162. Epub, 2019 Aug 15. PMID:31334636<ref>PMID:31334636</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6kj1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Li, D]]
		+	[[Category: Li, X]]
		+	[[Category: Liu, C]]
		+	[[Category: Luo, F]]
		+	[[Category: Luo, Z]]
		+	[[Category: Zhou, H]]
		+	[[Category: Fus]]
		+	[[Category: Microed]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Ultrahigh resolution]]

OCA: Protected "6kj1" [edit=sysop:move=sysop]

OCA — Wed, 31 Jul 2019 04:35:55 GMT

Protected "6kj1" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:35, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6kj1 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Jul 2019 04:35:54 GMT

New page: '''Unreleased structure''' The entry 6kj1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6kj1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]