6kja - Revision history

OCA at 10:34, 22 November 2023

OCA — Wed, 22 Nov 2023 10:34:42 GMT

←Older revision		Revision as of 10:34, 22 November 2023
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	<StructureSection load='6kja' size='340' side='right'caption='[[6kja]], [[Resolution\|resolution]] 3.06Å' scene=''>		<StructureSection load='6kja' size='340' side='right'caption='[[6kja]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kja]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KJA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kja]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KJA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.064Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>pyrB, b4245, JW4204 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83333 ECOLI]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kja OCA], [https://pdbe.org/6kja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kja RCSB], [https://www.ebi.ac.uk/pdbsum/6kja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kja ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kja OCA], [~~http~~://pdbe.org/6kja PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kja RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kja PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kja ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~PYRI_ECOLI PYRI_ECOLI]] Involved in allosteric regulation of aspartate carbamoyltransferase.[HAMAP-Rule:MF_00002~~]	+	[https://www.uniprot.org/uniprot/PYRB_ECOLI PYRB_ECOLI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6kja" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6kja" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aspartate carbamoyltransferase 3D structures\|Aspartate carbamoyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aspartate carbamoyltransferase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jia~~, Z C~~]]	+	[[Category: Jia ZC]]
-	[[Category: Lei, Z]]	+	[[Category: Lei Z]]
-	[[Category: Zheng, J]]	+	[[Category: Zheng J]]
-	~~[[Category: Aspartate transcarbamoylase holoenzyme]]~~	+
-	~~[[Category: De novo pyrimidine biosynthesis]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:22, 26 August 2020

OCA — Wed, 26 Aug 2020 11:22:19 GMT

←Older revision		Revision as of 11:22, 26 August 2020
Line 3:		Line 3:
	<StructureSection load='6kja' size='340' side='right'caption='[[6kja]], [[Resolution\|resolution]] 3.06Å' scene=''>		<StructureSection load='6kja' size='340' side='right'caption='[[6kja]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kja]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJA OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=6KJA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kja]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KJA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pyrB, b4245, JW4204 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), pyrI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6kja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kja OCA], [http://pdbe.org/6kja PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kja RCSB], [http://www.ebi.ac.uk/pdbsum/6kja PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kja ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kja OCA], [http://pdbe.org/6kja PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kja RCSB], [http://www.ebi.ac.uk/pdbsum/6kja PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kja ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Aspartate carbamoyltransferase]]		[[Category: Aspartate carbamoyltransferase]]
		+	[[Category: Ecoli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jia, Z C]]		[[Category: Jia, Z C]]

OCA at 07:15, 11 March 2020

OCA — Wed, 11 Mar 2020 07:15:53 GMT

←Older revision		Revision as of 07:15, 11 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6kja is ~~ON HOLD~~	+	==E. coli ATCase holoenzyme mutant - G128/130A (catalytic chain)==
		+	<StructureSection load='6kja' size='340' side='right'caption='[[6kja]], [[Resolution\|resolution]] 3.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6kja]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KJA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aspartate_carbamoyltransferase Aspartate carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.2 2.1.3.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kja OCA], [http://pdbe.org/6kja PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kja RCSB], [http://www.ebi.ac.uk/pdbsum/6kja PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kja ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PYRI_ECOLI PYRI_ECOLI]] Involved in allosteric regulation of aspartate carbamoyltransferase.[HAMAP-Rule:MF_00002]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aspartate transcarbamoylase (ATCase) is a key enzyme which regulates and catalyzes the second step of de novo pyrimidine synthesis in all organisms. Escherichia coli ATCase is a prototypic enzyme regulated by both product feedback and substrate cooperativity, whereas human ATCase is a potential anticancer target. Through structural and biochemical analyses, we revealed that R167/130's loop region in ATCase serves as a gatekeeper for the active site, playing a new and unappreciated regulatory role in the catalytic cycle of ATCase. Based on virtual compound screening simultaneously targeting the new regulatory region and active site of human ATCase, two compounds were identified to exhibit strong inhibition of ATCase activity, proliferation of multiple cancer cell lines, and growth of xenograft tumors. Our work has not only revealed a previously unknown regulatory region of ATCase that helps uncover the catalytic and regulatory mechanism of ATCase, but also successfully guided the identification of new ATCase inhibitors for anticancer drug development using a dual-targeting strategy. DATABASE: Structure data are available in Protein Data Bank under the accession numbers: 6KJ7 (G166P ecATCase), 6KJ8 (G166P ecATCase-holo), 6KJ9 (G128/130A ecATCase), and 6KJA (G128/130A ecATCase-holo).

-	~~Authors:~~ Lei, Z., ~~Zheng~~, J., Jia, Z.C.	+	New regulatory mechanism-based inhibitors of aspartate transcarbamoylase for potential anticancer drug development.,Lei Z, Wang B, Lu Z, Wang N, Tan H, Zheng J, Jia Z FEBS J. 2020 Jan 22. doi: 10.1111/febs.15220. PMID:31967710<ref>PMID:31967710</ref>

-	~~Description: E~~. ~~coli ATCase holoenzyme mutant~~ -~~G128~~/~~130A (catalytic chain)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Jia, Z.C]]	+	<div class="pdbe-citations 6kja" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aspartate carbamoyltransferase]]
		+	[[Category: Large Structures]]
		+	[[Category: Jia, Z C]]
	[[Category: Lei, Z]]		[[Category: Lei, Z]]
	[[Category: Zheng, J]]		[[Category: Zheng, J]]
		+	[[Category: Aspartate transcarbamoylase holoenzyme]]
		+	[[Category: De novo pyrimidine biosynthesis]]
		+	[[Category: Transferase]]

OCA at 06:29, 1 January 2020

OCA — Wed, 01 Jan 2020 06:29:57 GMT

←Older revision		Revision as of 06:29, 1 January 2020
Line 3:		Line 3:
	The entry 6kja is ON HOLD		The entry 6kja is ON HOLD

-	Authors: ~~Zhen~~, L., ~~Jimin~~, Z., ~~Zongchao~~, J.	+	Authors: Lei, Z., Zheng, J., Jia, Z.C.

	Description: E. coli ATCase holoenzyme mutant -G128/130A (catalytic chain)		Description: E. coli ATCase holoenzyme mutant -G128/130A (catalytic chain)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Jimin~~, Z]]	+	[[Category: Jia, Z.C]]
-	[[Category: ~~Zhen~~, L]]	+	[[Category: Lei, Z]]
-	[[Category: ~~Zongchao~~, J]]	+	[[Category: Zheng, J]]

OCA: Protected "6kja" [edit=sysop:move=sysop]

OCA — Wed, 31 Jul 2019 04:36:31 GMT

Protected "6kja" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:36, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6kja is ON HOLD Authors: Zhen, L., Jimin, Z., Zongchao, J. Description: E. coli ATCase holoenzyme mutant -G128/130A (catalytic chain) [[Category: U...

OCA — Wed, 31 Jul 2019 04:36:30 GMT

New page: '''Unreleased structure''' The entry 6kja is ON HOLD Authors: Zhen, L., Jimin, Z., Zongchao, J. Description: E. coli ATCase holoenzyme mutant -G128/130A (catalytic chain) [[Category: U...

New page

'''Unreleased structure'''

The entry 6kja is ON HOLD

Authors: Zhen, L., Jimin, Z., Zongchao, J.

Description: E. coli ATCase holoenzyme mutant -G128/130A (catalytic chain)
[[Category: Unreleased Structures]]
[[Category: Jimin, Z]]
[[Category: Zhen, L]]
[[Category: Zongchao, J]]