6kjn - Revision history

OCA at 10:53, 14 June 2023

OCA — Wed, 14 Jun 2023 10:53:23 GMT

←Older revision		Revision as of 10:53, 14 June 2023
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	==The microtubule-binding domains of yeast cytoplasmic dynein in the high affinity state==		==The microtubule-binding domains of yeast cytoplasmic dynein in the high affinity state==
-	<StructureSection load='6kjn' size='340' side='right'caption='[[6kjn~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='6kjn' size='340' side='right'caption='[[6kjn]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kjn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6KJN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kjn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KJN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6kjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kjn OCA], [~~http~~://pdbe.org/6kjn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kjn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kjn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kjn ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kjn OCA], [https://pdbe.org/6kjn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kjn RCSB], [https://www.ebi.ac.uk/pdbsum/6kjn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kjn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST]] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref>	+	[https://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6kjn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6kjn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dynein 3D structures\|Dynein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kikkawa, M]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Komori, Y]]	+	[[Category: Kikkawa M]]
-	[[Category: Kubo, S]]	+	[[Category: Komori Y]]
-	[[Category: Nishida, N]]	+	[[Category: Kubo S]]
-	[[Category: Shimada, I]]	+	[[Category: Nishida N]]
-	[[Category: Takarada, O]]	+	[[Category: Shimada I]]
-	[[Category: Tamura, S]]	+	[[Category: Takarada O]]
-	[[Category: Watanabe, A]]	+	[[Category: Tamura S]]
-	~~[[Category: Disulfide bond]]~~	+	[[Category: Watanabe A]]
-	~~[[Category: Dynein]]~~	+
-	~~[[Category: High affinity]]~~	+
-	~~[[Category: Microtubule]]~~	+
-	~~[[Category: Motor protein~~]]	+

OCA at 09:39, 18 March 2020

OCA — Wed, 18 Mar 2020 09:39:36 GMT

←Older revision		Revision as of 09:39, 18 March 2020
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-	'''Unreleased structure'''

-	The ~~entry~~ 6kjn is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==The microtubule-binding domains of yeast cytoplasmic dynein in the high affinity state==
		+	<StructureSection load='6kjn' size='340' side='right'caption='[[6kjn]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6kjn]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KJN FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kjn OCA], [http://pdbe.org/6kjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kjn RCSB], [http://www.ebi.ac.uk/pdbsum/6kjn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kjn ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST]] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The movements of cytoplasmic dynein on microtubule (MT) tracks is achieved by two-way communication between the microtubule-binding domain (MTBD) and the ATPase domain via a coiled-coil stalk, but the structural basis of this communication remains elusive. Here, we regulate MTBD either in high-affinity or low-affinity states by introducing a disulfide bond to the stalk and analyze the resulting structures by NMR and cryo-EM. In the MT-unbound state, the affinity changes of MTBD are achieved by sliding of the stalk alpha-helix by a half-turn, which suggests that structural changes propagate from the ATPase-domain to MTBD. In addition, MT binding induces further sliding of the stalk alpha-helix even without the disulfide bond, suggesting how the MT-induced conformational changes propagate toward the ATPase domain. Based on differences in the MT-binding surface between the high- and low-affinity states, we propose a potential mechanism for the directional bias of dynein movement on MT tracks.

-	~~Authors~~:	+	Structural basis for two-way communication between dynein and microtubules.,Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M Nat Commun. 2020 Feb 25;11(1):1038. doi: 10.1038/s41467-020-14842-8. PMID:32098965<ref>PMID:32098965</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6kjn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kikkawa, M]]
		+	[[Category: Komori, Y]]
		+	[[Category: Kubo, S]]
		+	[[Category: Nishida, N]]
		+	[[Category: Shimada, I]]
		+	[[Category: Takarada, O]]
		+	[[Category: Tamura, S]]
		+	[[Category: Watanabe, A]]
		+	[[Category: Disulfide bond]]
		+	[[Category: Dynein]]
		+	[[Category: High affinity]]
		+	[[Category: Microtubule]]
		+	[[Category: Motor protein]]

OCA at 04:06, 7 August 2019

OCA — Wed, 07 Aug 2019 04:06:27 GMT

←Older revision		Revision as of 04:06, 7 August 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kjn is ON HOLD	+	The entry 6kjn is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6kjn" [edit=sysop:move=sysop]

OCA — Wed, 31 Jul 2019 04:37:37 GMT

Protected "6kjn" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:37, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6kjn is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Jul 2019 04:37:35 GMT

New page: '''Unreleased structure''' The entry 6kjn is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6kjn is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]