OCA at 10:35, 22 November 2023

OCA — Wed, 22 Nov 2023 10:35:32 GMT

←Older revision		Revision as of 10:35, 22 November 2023
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	==The crystal structure of apo-SiaC from Pseudomonas aeruginosa==		==The crystal structure of apo-SiaC from Pseudomonas aeruginosa==
-	<StructureSection load='6kkp' size='340' side='right'caption='[[6kkp]]' scene=''>	+	<StructureSection load='6kkp' size='340' side='right'caption='[[6kkp]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KKP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KKP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kkp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KKP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kkp OCA], [~~http~~://pdbe.org/6kkp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kkp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kkp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kkp ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kkp OCA], [https://pdbe.org/6kkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kkp RCSB], [https://www.ebi.ac.uk/pdbsum/6kkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kkp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9I6W2_PSEAE Q9I6W2_PSEAE]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial cyclic-di-GMP (c-di-GMP) production is associated with biofilm development and the switch from acute to chronic infections. In Pseudomonas aeruginosa, the diguanylate cyclase (DGC) SiaD and phosphatase SiaA, which are co-transcribed as part of a siaABCD operon, are essential for cellular aggregation. However, the detailed functions of this operon and the relationships among its constituent genes are unknown. Here, we demonstrate that the siaABCD operon encodes for a signaling network that regulates SiaD enzymatic activity to control biofilm and aggregates formation. Through protein-protein interaction, SiaC promotes SiaD diguanylate cyclase activity. Biochemical and structural data revealed that SiaB is an unusual protein kinase that phosphorylates SiaC, whereas SiaA phosphatase can dephosphorylate SiaC. The phosphorylation state of SiaC is critical for its interaction with SiaD, which will switch on or off the DGC activity of SiaD and regulate c-di-GMP levels and subsequent virulence phenotypes. Collectively, our data provide insights into the molecular mechanisms underlying the modulation of DGC activity associated with chronic infections, which may facilitate the development of antimicrobial drugs.
		+
		+	The SiaA/B/C/D signaling network regulates biofilm formation in Pseudomonas aeruginosa.,Chen G, Gan J, Yang C, Zuo Y, Peng J, Li M, Huo W, Xie Y, Zhang Y, Wang T, Deng X, Liang H EMBO J. 2020 Mar 16;39(6):e103412. doi: 10.15252/embj.2019103412. Epub 2020 Feb, 24. PMID:32090355<ref>PMID:32090355</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kkp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudomonas aeruginosa]]
	[[Category: Chen GK]]		[[Category: Chen GK]]
	[[Category: Gan JH]]		[[Category: Gan JH]]
	[[Category: Liang HH]]		[[Category: Liang HH]]
	[[Category: Yang C]]		[[Category: Yang C]]

OCA at 06:22, 10 June 2020

OCA — Wed, 10 Jun 2020 06:22:44 GMT

←Older revision		Revision as of 06:22, 10 June 2020
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-	'''Unreleased structure'''

-	The ~~entry~~ 6kkp is ~~ON HOLD until Paper Publication~~	+	==The crystal structure of apo-SiaC from Pseudomonas aeruginosa==
-		+	<StructureSection load='6kkp' size='340' side='right'caption='[[6kkp]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KKP OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KKP FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kkp OCA], [http://pdbe.org/6kkp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kkp RCSB], [http://www.ebi.ac.uk/pdbsum/6kkp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kkp ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chen GK]]
		+	[[Category: Gan JH]]
		+	[[Category: Liang HH]]
		+	[[Category: Yang C]]

OCA at 04:01, 21 August 2019

OCA — Wed, 21 Aug 2019 04:01:40 GMT

←Older revision		Revision as of 04:01, 21 August 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kkp is ON HOLD	+	The entry 6kkp is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6kkp" [edit=sysop:move=sysop]

OCA — Wed, 31 Jul 2019 04:40:08 GMT

Protected "6kkp" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:40, 31 July 2019

OCA: New page: '''Unreleased structure''' The entry 6kkp is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Jul 2019 04:40:06 GMT

New page: '''Unreleased structure''' The entry 6kkp is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6kkp is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6kkp - Revision history

OCA at 10:35, 22 November 2023

OCA at 06:22, 10 June 2020

OCA at 04:01, 21 August 2019

OCA: Protected "6kkp" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6kkp is ON HOLD Authors: Description: Category: Unreleased Structures