6kou - Revision history

OCA at 10:43, 27 March 2024

OCA — Wed, 27 Mar 2024 10:43:38 GMT

←Older revision		Revision as of 10:43, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6kou' size='340' side='right'caption='[[6kou]], [[Resolution\|resolution]] 2.43Å' scene=''>		<StructureSection load='6kou' size='340' side='right'caption='[[6kou]], [[Resolution\|resolution]] 2.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kou]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KOU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6KOU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kou]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KOU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.43Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6kou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kou OCA], [~~http~~://pdbe.org/6kou PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kou RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kou PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kou ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kou OCA], [https://pdbe.org/6kou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kou RCSB], [https://www.ebi.ac.uk/pdbsum/6kou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kou ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/ILVC2_SACS2 ILVC2_SACS2]
-	Protein functions are temperature-dependent, but protein structures are usually solved at a single (often low) temperature because of limitations on the conditions of crystal growth or protein vitrification. Here we demonstrate the feasibility of solving cryo-EM structures of proteins vitrified at high temperatures, solve 12 structures of an archaeal ketol-acid reductoisomerase (KARI) vitrified at 4-70 degrees C, and show that structures of both the Mg(2+) form (KARI:2Mg(2+)) and its ternary complex (KARI:2Mg(2+):NADH:inhibitor) are temperature-dependent in correlation with the temperature dependence of enzyme activity. Furthermore, structural analyses led to dissection of the induced-fit mechanism into ligand-induced and temperature-induced effects and to capture of temperature-resolved intermediates of the temperature-induced conformational change. The results also suggest that it is preferable to solve cryo-EM structures of protein complexes at functional temperatures. ~~These studies should greatly expand the landscapes of protein structure-function relationships and enhance the mechanistic analysis of enzymatic functions~~.	+

-	Temperature-Resolved Cryo-EM Uncovers Structural Bases of Temperature-Dependent Enzyme Functions.,Chen CY, Chang YC, Lin BL, Huang CH, Tsai MD J Am Chem Soc. 2019 Dec 26;141(51):19983-19987. doi: 10.1021/jacs.9b10687. Epub, 2019 Dec 16. PMID:31829582<ref>PMID:31829582</ref>	+	==See Also==
-		+	*[[Ketol-acid reductoisomerase 3D structures\|Ketol-acid reductoisomerase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 6kou" style~~=~~"background-color:#fffaf0;"></div>~~	+
-	~~== References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chang, Y C~~]]	+	[[Category: Saccharolobus solfataricus]]
-	[[Category: ~~Chen, C Y~~]]	+	[[Category: Chang YC]]
-	[[Category: ~~Huang, C H~~]]	+	[[Category: Chen CY]]
-	[[Category: ~~Lin, B L~~]]	+	[[Category: Huang CH]]
-	[[Category: ~~Tsai, M D~~]]	+	[[Category: Lin BL]]
-	[[Category: ~~Complex]]~~	+	[[Category: Tsai MD]]
-	~~[[Category: Isomerase~~]]	+

OCA at 10:06, 27 March 2020

OCA — Fri, 27 Mar 2020 10:06:38 GMT

←Older revision		Revision as of 10:06, 27 March 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6kou is ~~ON HOLD until Paper~~ Publication	+	==277 K cryoEM structure of Sso-KARI in complex with magnesium ions==
		+	<StructureSection load='6kou' size='340' side='right'caption='[[6kou]], [[Resolution\|resolution]] 2.43Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6kou]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KOU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KOU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kou OCA], [http://pdbe.org/6kou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kou RCSB], [http://www.ebi.ac.uk/pdbsum/6kou PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kou ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein functions are temperature-dependent, but protein structures are usually solved at a single (often low) temperature because of limitations on the conditions of crystal growth or protein vitrification. Here we demonstrate the feasibility of solving cryo-EM structures of proteins vitrified at high temperatures, solve 12 structures of an archaeal ketol-acid reductoisomerase (KARI) vitrified at 4-70 degrees C, and show that structures of both the Mg(2+) form (KARI:2Mg(2+)) and its ternary complex (KARI:2Mg(2+):NADH:inhibitor) are temperature-dependent in correlation with the temperature dependence of enzyme activity. Furthermore, structural analyses led to dissection of the induced-fit mechanism into ligand-induced and temperature-induced effects and to capture of temperature-resolved intermediates of the temperature-induced conformational change. The results also suggest that it is preferable to solve cryo-EM structures of protein complexes at functional temperatures. These studies should greatly expand the landscapes of protein structure-function relationships and enhance the mechanistic analysis of enzymatic functions.

-	~~Authors:~~ Chen~~, C.Y.~~, Chang~~, Y.C.~~, Lin~~, B.L.~~, Huang, C.H., ~~Tsai, M.D~~.	+	Temperature-Resolved Cryo-EM Uncovers Structural Bases of Temperature-Dependent Enzyme Functions.,Chen CY, Chang YC, Lin BL, Huang CH, Tsai MD J Am Chem Soc. 2019 Dec 26;141(51):19983-19987. doi: 10.1021/jacs.9b10687. Epub, 2019 Dec 16. PMID:31829582<ref>PMID:31829582</ref>

-	~~Description: 277 K cryoEM structure~~ of ~~Sso~~-~~KARI in complex with magnesium ions~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Huang~~, C.H]]	+	<div class="pdbe-citations 6kou" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Chang~~, Y.C]]	+	== References ==
-	[[Category: ~~Chen~~, C.Y]]	+	<references/>
-	[[Category: Tsai, M.D]]	+	__TOC__
-	[[Category: ~~Lin, B.L~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chang, Y C]]
		+	[[Category: Chen, C Y]]
		+	[[Category: Huang, C H]]
		+	[[Category: Lin, B L]]
		+	[[Category: Tsai, M D]]
		+	[[Category: Complex]]
		+	[[Category: Isomerase]]

OCA at 06:36, 2 October 2019

OCA — Wed, 02 Oct 2019 06:36:47 GMT

←Older revision		Revision as of 06:36, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kou is ON HOLD	+	The entry 6kou is ON HOLD until Paper Publication

	Authors: Chen, C.Y., Chang, Y.C., Lin, B.L., Huang, C.H., Tsai, M.D.		Authors: Chen, C.Y., Chang, Y.C., Lin, B.L., Huang, C.H., Tsai, M.D.

OCA: Protected "6kou" [edit=sysop:move=sysop]

OCA — Wed, 21 Aug 2019 04:04:34 GMT

Protected "6kou" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:04, 21 August 2019

OCA: New page: '''Unreleased structure''' The entry 6kou is ON HOLD Authors: Chen, C.Y., Chang, Y.C., Lin, B.L., Huang, C.H., Tsai, M.D. Description: 277 K cryoEM structure of Sso-KARI in complex wit...

OCA — Wed, 21 Aug 2019 04:04:32 GMT

New page: '''Unreleased structure''' The entry 6kou is ON HOLD Authors: Chen, C.Y., Chang, Y.C., Lin, B.L., Huang, C.H., Tsai, M.D. Description: 277 K cryoEM structure of Sso-KARI in complex wit...

New page

'''Unreleased structure'''

The entry 6kou is ON HOLD

Authors: Chen, C.Y., Chang, Y.C., Lin, B.L., Huang, C.H., Tsai, M.D.

Description: 277 K cryoEM structure of Sso-KARI in complex with magnesium ions
[[Category: Unreleased Structures]]
[[Category: Huang, C.H]]
[[Category: Chang, Y.C]]
[[Category: Chen, C.Y]]
[[Category: Tsai, M.D]]
[[Category: Lin, B.L]]