OCA at 10:40, 22 November 2023

OCA — Wed, 22 Nov 2023 10:40:16 GMT

←Older revision		Revision as of 10:40, 22 November 2023
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	==A pre-assembled molecular-helical Cascade backbone of Csy3 subunits from Zymomonas mobilis==		==A pre-assembled molecular-helical Cascade backbone of Csy3 subunits from Zymomonas mobilis==
-	<StructureSection load='6kqr' size='340' side='right'caption='[[6kqr]]' scene=''>	+	<StructureSection load='6kqr' size='340' side='right'caption='[[6kqr]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KQR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KQR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kqr]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Zymomonas_mobilis_subsp._mobilis Zymomonas mobilis subsp. mobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KQR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kqr OCA], [~~http~~://pdbe.org/6kqr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kqr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kqr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kqr ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.899Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kqr OCA], [https://pdbe.org/6kqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kqr RCSB], [https://www.ebi.ac.uk/pdbsum/6kqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kqr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5NPQ2_ZYMMO Q5NPQ2_ZYMMO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The currently known prokaryotic adaptive immune system against mobile genetic elements is based on clustered regularly interspaced short palindromic repeats (CRISPR). CRISPR-associated (Cas) proteins and the transcribed short CRISPR RNA (crRNA) molecule form a heterologous ribonucleoprotein complex that neutralizes invading foreign nucleic acids, wherein the crRNA molecule base-pairs with the exogenous genetic elements. In the ribonucleoprotein complexes of the type I CRISPR system, a helical backbone of six identical subunits is commonly found. However, it is not clear how this ribonucleoprotein complex is assembled and what is the determinant factor for its size. We elucidated the crystal structure of the Csy3 subunit of the type I-F ribonucleoprotein complex from Zymomonas mobilis (ZmCsy3), in which seven ZmCsy3 protomers in the asymmetric unit form a molecular helix that is part of a filamentous structure in the entire crystal system. This ZmCsy3 helical structure is remarkably similar to the crRNA-bound hexameric Csy3 backbone from Pseudomonas aeruginosa, with conserved interactions between neighboring subunits. The monomeric ZmCsy3 in solution is transformed into different oligomeric states depending on the added crRNAs. These results suggest that a crRNA and Csy3 subunit play a determinant role in the stepwise formation of the functional Cascade ribonucleoprotein complex and the recruitment of other subunits, and crRNA functions as a molecular ruler for determining the size of the Cascade silencing complex.
		+
		+	A CRISPR RNA Is Closely Related With the Size of the Cascade Nucleoprotein Complex.,Gu DH, Ha SC, Kim JS Front Microbiol. 2019 Oct 29;10:2458. doi: 10.3389/fmicb.2019.02458. eCollection , 2019. PMID:31736904<ref>PMID:31736904</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kqr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Zymomonas mobilis subsp. mobilis]]
	[[Category: Gu DH]]		[[Category: Gu DH]]
	[[Category: Ha SC]]		[[Category: Ha SC]]
	[[Category: Kim JS]]		[[Category: Kim JS]]

OCA at 05:25, 13 May 2020

OCA — Wed, 13 May 2020 05:25:01 GMT

←Older revision		Revision as of 05:25, 13 May 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6kqr is ~~ON HOLD~~	+	==A pre-assembled molecular-helical Cascade backbone of Csy3 subunits from Zymomonas mobilis==
-		+	<StructureSection load='6kqr' size='340' side='right'caption='[[6kqr]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KQR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KQR FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kqr OCA], [http://pdbe.org/6kqr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kqr RCSB], [http://www.ebi.ac.uk/pdbsum/6kqr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kqr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gu DH]]
		+	[[Category: Ha SC]]
		+	[[Category: Kim JS]]

OCA: Protected "6kqr" [edit=sysop:move=sysop]

OCA — Wed, 28 Aug 2019 13:37:22 GMT

Protected "6kqr" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:37, 28 August 2019

OCA: New page: '''Unreleased structure''' The entry 6kqr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Aug 2019 13:37:20 GMT

New page: '''Unreleased structure''' The entry 6kqr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6kqr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6kqr - Revision history

OCA at 10:40, 22 November 2023

OCA at 05:25, 13 May 2020

OCA: Protected "6kqr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6kqr is ON HOLD Authors: Description: Category: Unreleased Structures