OCA at 06:20, 19 February 2020

OCA — Wed, 19 Feb 2020 06:20:42 GMT

←Older revision		Revision as of 06:20, 19 February 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6kv2 is ~~ON HOLD~~	+	==Crystal structure of trypsin inhibitor 1 from Senna obtusifolia==
		+	<StructureSection load='6kv2' size='340' side='right'caption='[[6kv2]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6kv2]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KV2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KV2 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxalate_oxidase Oxalate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.3.4 1.2.3.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kv2 OCA], [http://pdbe.org/6kv2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kv2 RCSB], [http://www.ebi.ac.uk/pdbsum/6kv2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kv2 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although digestive resistance of Kunitz protease inhibitors has been reported extensively, the molecular mechanism is not well established. In the present study, the first X-ray structure of Cassia obtusifolia trypsin inhibitor (COTI), a member of Kunitz protease inhibitors, was solved at a resolution of 1.9 A. The structure adopted a classic beta-trefoil fold with the inhibitory loop protruding from the hydrophobic core. The role of Phe139, a unique residue in Kunitz protease inhibitors, and Arg63 in the COTI structure was verified by F139A and R63E mutants. COTI was a specific inhibitor of bovine trypsin and the result was also verified by COTI-trypsin complex formation. Meanwhile, COTI showed equivalent inhibitory activity with that of soybean trypsin inhibitor against bovine trypsin and midgut trypsin from Pieris rapae. The F139 and R63E mutants further indicated that inhibitory specificity and efficiency of COTI were closely related to the global framework, the conformation and the amino acid composition of reactive loop. Finally, a midgut trypsin from P. rapae (PrSP40), which might be involve in the food digestion, was proposed to be a potential target of COTI and might be a promising target for future crop-protection strategy. The results supported the digestive resistance of COTI.

-	~~Authors: Liao~~, H., Yu, Y.	+	Structural and functional relationship of Cassia obtusifolia trypsin inhibitor to understand its digestive resistance against Pieris rapae.,Zhou J, Li C, Chen A, Zhu J, Zou M, Liao H, Yu Y Int J Biol Macromol. 2020 Jan 22;148:908-920. doi:, 10.1016/j.ijbiomac.2020.01.193. PMID:31981663<ref>PMID:31981663</ref>

-	~~Description: Crystal structure~~ of ~~trypsin inhibitor 1 from Senna obtusifolia~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Yu, Y~~]]	+	<div class="pdbe-citations 6kv2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Oxalate oxidase]]
	[[Category: Liao, H]]		[[Category: Liao, H]]
		+	[[Category: Yu, Y]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Trypsin inhibitor]]

OCA: Protected "6kv2" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 06:49:07 GMT

Protected "6kv2" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:49, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kv2 is ON HOLD Authors: Liao, H., Yu, Y. Description: Crystal structure of trypsin inhibitor 1 from Senna obtusifolia [[Category: Unreleased Struc...

OCA — Wed, 02 Oct 2019 06:49:05 GMT

New page: '''Unreleased structure''' The entry 6kv2 is ON HOLD Authors: Liao, H., Yu, Y. Description: Crystal structure of trypsin inhibitor 1 from Senna obtusifolia [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 6kv2 is ON HOLD

Authors: Liao, H., Yu, Y.

Description: Crystal structure of trypsin inhibitor 1 from Senna obtusifolia
[[Category: Unreleased Structures]]
[[Category: Yu, Y]]
[[Category: Liao, H]]

6kv2 - Revision history

OCA at 06:20, 19 February 2020

OCA: Protected "6kv2" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6kv2 is ON HOLD Authors: Liao, H., Yu, Y. Description: Crystal structure of trypsin inhibitor 1 from Senna obtusifolia [[Category: Unreleased Struc...