6kvk - Revision history

OCA at 10:44, 22 November 2023

OCA — Wed, 22 Nov 2023 10:44:27 GMT

←Older revision		Revision as of 10:44, 22 November 2023
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	<StructureSection load='6kvk' size='340' side='right'caption='[[6kvk]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6kvk' size='340' side='right'caption='[[6kvk]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kvk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eupatorium_rebaudianum Eupatorium rebaudianum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KVK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stevia_rebaudiana Stevia rebaudiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KVK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DX0:Steviolmonoside'>DX0</scene>~~, ~~<scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.397Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~UGT76G1 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=55670 Eupatorium rebaudianum])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DX0:Steviolmonoside'>DX0</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvk OCA], [~~http~~://pdbe.org/6kvk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kvk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kvk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvk OCA], [https://pdbe.org/6kvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kvk RCSB], [https://www.ebi.ac.uk/pdbsum/6kvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvk ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/U76G1_STERE U76G1_STERE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Eupatorium rebaudianum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Li, J X]]
-	[[Category: Liu, Z F]]
-	[[Category: Wang, Y]]
-	[[Category: Zhang, P]]
-	[[Category: Diterpenoid]]
-	[[Category: Glycosyltransferase]]
-	[[Category: Natural sweetener]]
	[[Category: Stevia rebaudiana]]		[[Category: Stevia rebaudiana]]
-	[[Category: ~~Transferase~~]]	+	[[Category: Li JX]]
		+	[[Category: Liu ZF]]
		+	[[Category: Wang Y]]
		+	[[Category: Zhang P]]

OCA at 08:17, 20 January 2021

OCA — Wed, 20 Jan 2021 08:17:08 GMT

←Older revision		Revision as of 08:17, 20 January 2021
Line 3:		Line 3:
	<StructureSection load='6kvk' size='340' side='right'caption='[[6kvk]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6kvk' size='340' side='right'caption='[[6kvk]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kvk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6KVK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kvk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Eupatorium_rebaudianum Eupatorium rebaudianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KVK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DX0:Steviolmonoside'>DX0</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DX0:Steviolmonoside'>DX0</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvk OCA], [http://pdbe.org/6kvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kvk RCSB], [http://www.ebi.ac.uk/pdbsum/6kvk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvk ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UGT76G1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55670 Eupatorium rebaudianum])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvk OCA], [http://pdbe.org/6kvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kvk RCSB], [http://www.ebi.ac.uk/pdbsum/6kvk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvk ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Diterpene glycosyltransferase UGT76G1 from Stevia rebaudiana (SrUGT76G1) is key to the generation of economically important steviol glycosides (SGs), a group of natural sweeteners with high-intensity sweetness. SrUGT76G1 accommodates a wide range of steviol-derived substrates and many other small molecules. We report here the crystal structures of SrUGT76G1 in complex with multiple ligands to answer how this enzyme recognizes diterpenoid aglycones and catalyzes the 1,3-sugar chain branching. A spacious pocket for sugar-acceptor binding was observed from the determined SrUGT76G1 structures, which can explain its broad substrate spectrum. Residues Gly87 and Leu204 lining the pocket play key roles in switching between diterpenoid and flavonoid glucosylation. An engineered mutant of SrUGT76G1, T284S, could catalyze a selectively increased production of next-generation sweetener rebaudioside M, with diminished side product of rebaudioside I. Taken together, these resutls provide significant insights into molecular basis of the substrate specificity of scarcely documented diterpenoid glycosyltransferases and would facilitate the structure-guided glycoengineering to produce diversified diterpenoids with new activities.
		+
		+	Structural Insights into the Catalytic Mechanism of a Plant Diterpene Glycosyltransferase SrUGT76G1.,Liu Z, Li J, Sun Y, Zhang P, Wang Y Plant Commun. 2019 Sep 28;1(1):100004. doi: 10.1016/j.xplc.2019.100004., eCollection 2020 Jan 13. PMID:33404544<ref>PMID:33404544</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kvk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Eupatorium rebaudianum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Li, J X]]		[[Category: Li, J X]]

OCA at 15:23, 20 November 2019

OCA — Wed, 20 Nov 2019 15:23:19 GMT

←Older revision		Revision as of 15:23, 20 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6kvk is ~~ON HOLD~~	+	==Crystal structure of UDP-Sm-SrUGT76G1==
-		+	<StructureSection load='6kvk' size='340' side='right'caption='[[6kvk]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~Authors~~: Li, J.X., ~~Liu~~, Z.F., ~~Wang, Y~~., ~~Zhang, P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6kvk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KVK FirstGlance]. <br>
-	~~Description: Crystal structure of UDP-Sm-SrUGT76G1~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DX0:Steviolmonoside'>DX0</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvk OCA], [http://pdbe.org/6kvk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kvk RCSB], [http://www.ebi.ac.uk/pdbsum/6kvk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvk ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Li, J X]]
		+	[[Category: Liu, Z F]]
		+	[[Category: Wang, Y]]
	[[Category: Zhang, P]]		[[Category: Zhang, P]]
-	[[Category: ~~Wang, Y~~]]	+	[[Category: Diterpenoid]]
-	[[Category: ~~Liu, Z.F~~]]	+	[[Category: Glycosyltransferase]]
-	[[Category: ~~Li, J.X~~]]	+	[[Category: Natural sweetener]]
		+	[[Category: Stevia rebaudiana]]
		+	[[Category: Transferase]]

OCA at 04:59, 6 November 2019

OCA — Wed, 06 Nov 2019 04:59:20 GMT

←Older revision		Revision as of 04:59, 6 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kvk is ON HOLD ~~until Paper Publication~~	+	The entry 6kvk is ON HOLD

	Authors: Li, J.X., Liu, Z.F., Wang, Y., Zhang, P.		Authors: Li, J.X., Liu, Z.F., Wang, Y., Zhang, P.

OCA at 07:17, 30 October 2019

OCA — Wed, 30 Oct 2019 07:17:11 GMT

←Older revision		Revision as of 07:17, 30 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kvk is ON HOLD	+	The entry 6kvk is ON HOLD until Paper Publication

	Authors: Li, J.X., Liu, Z.F., Wang, Y., Zhang, P.		Authors: Li, J.X., Liu, Z.F., Wang, Y., Zhang, P.
Line 7:		Line 7:
	Description: Crystal structure of UDP-Sm-SrUGT76G1		Description: Crystal structure of UDP-Sm-SrUGT76G1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Li, J.X]]
	[[Category: Zhang, P]]		[[Category: Zhang, P]]
	[[Category: Wang, Y]]		[[Category: Wang, Y]]
	[[Category: Liu, Z.F]]		[[Category: Liu, Z.F]]
		+	[[Category: Li, J.X]]

OCA: Protected "6kvk" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 06:51:04 GMT

Protected "6kvk" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:51, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kvk is ON HOLD Authors: Li, J.X., Liu, Z.F., Wang, Y., Zhang, P. Description: Crystal structure of UDP-Sm-SrUGT76G1 [[Category: Unreleased Structu...

OCA — Wed, 02 Oct 2019 06:51:03 GMT

New page: '''Unreleased structure''' The entry 6kvk is ON HOLD Authors: Li, J.X., Liu, Z.F., Wang, Y., Zhang, P. Description: Crystal structure of UDP-Sm-SrUGT76G1 [[Category: Unreleased Structu...

New page

'''Unreleased structure'''

The entry 6kvk is ON HOLD

Authors: Li, J.X., Liu, Z.F., Wang, Y., Zhang, P.

Description: Crystal structure of UDP-Sm-SrUGT76G1
[[Category: Unreleased Structures]]
[[Category: Li, J.X]]
[[Category: Zhang, P]]
[[Category: Wang, Y]]
[[Category: Liu, Z.F]]