6kvn - Revision history

OCA at 10:44, 27 March 2024

OCA — Wed, 27 Mar 2024 10:44:34 GMT

←Older revision		Revision as of 10:44, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6kvn' size='340' side='right'caption='[[6kvn]], [[Resolution\|resolution]] 2.02Å' scene=''>		<StructureSection load='6kvn' size='340' side='right'caption='[[6kvn]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kvn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KVN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kvn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KVN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvn OCA], [~~http~~://pdbe.org/6kvn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kvn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kvn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvn ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.02Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvn OCA], [https://pdbe.org/6kvn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kvn RCSB], [https://www.ebi.ac.uk/pdbsum/6kvn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvn ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A1S4CVP6_TOBAC A0A1S4CVP6_TOBAC]
-	Holliday junctions (HJs) are key DNA intermediates in genetic recombination and are eliminated by nuclease, termed resolvase, to ensure genome stability. HJ resolvases have been identified across all kingdoms of life, members of which exhibit sequence-dependent HJ resolution. However, the molecular basis of sequence selectivity remains largely unknown. Here, we present the chloroplast resolvase MOC1, which cleaves HJ in a cytosine-dependent manner. We determine the crystal structure of MOC1 with and without HJs. MOC1 exhibits an RNase H fold, belonging to the retroviral integrase family. MOC1 functions as a dimer, and the HJ is embedded into the basic cleft of the dimeric enzyme. We characterize a base recognition loop (BR loop) that protrudes into and opens the junction. Residues from the BR loop intercalate into the bases, disrupt the C-G base pairing at the crossover and recognize the cytosine, providing the molecular basis for sequence-dependent HJ resolution by a resolvase.	+

-	Structural insights into sequence-dependent Holliday junction resolution by the chloroplast resolvase MOC1.,Yan J, Hong S, Guan Z, He W, Zhang D, Yin P Nat Commun. 2020 Mar 17;11(1):1417. doi: 10.1038/s41467-020-15242-8. PMID:32184398<ref>PMID:32184398</ref>	+	==See Also==
-		+	*[[Resolvase 3D structures\|Resolvase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6kvn" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Guan, Z Y~~]]	+	[[Category: Nicotiana tabacum]]
-	[[Category: ~~Hong, S X~~]]	+	[[Category: Guan ZY]]
-	[[Category: ~~Yan, J J~~]]	+	[[Category: Hong SX]]
-	[[Category: ~~Yin, P~~]]	+	[[Category: Yan JJ]]
-	[[Category: ~~Chloroplast]]~~	+	[[Category: Yin P]]
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Resolvase~~]]	+

OCA at 06:48, 8 April 2020

OCA — Wed, 08 Apr 2020 06:48:30 GMT

←Older revision		Revision as of 06:48, 8 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6kvn is ~~ON HOLD~~	+	==Crystal structure of chloroplast resolvase==
		+	<StructureSection load='6kvn' size='340' side='right'caption='[[6kvn]], [[Resolution\|resolution]] 2.02Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6kvn]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KVN FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvn OCA], [http://pdbe.org/6kvn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kvn RCSB], [http://www.ebi.ac.uk/pdbsum/6kvn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvn ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Holliday junctions (HJs) are key DNA intermediates in genetic recombination and are eliminated by nuclease, termed resolvase, to ensure genome stability. HJ resolvases have been identified across all kingdoms of life, members of which exhibit sequence-dependent HJ resolution. However, the molecular basis of sequence selectivity remains largely unknown. Here, we present the chloroplast resolvase MOC1, which cleaves HJ in a cytosine-dependent manner. We determine the crystal structure of MOC1 with and without HJs. MOC1 exhibits an RNase H fold, belonging to the retroviral integrase family. MOC1 functions as a dimer, and the HJ is embedded into the basic cleft of the dimeric enzyme. We characterize a base recognition loop (BR loop) that protrudes into and opens the junction. Residues from the BR loop intercalate into the bases, disrupt the C-G base pairing at the crossover and recognize the cytosine, providing the molecular basis for sequence-dependent HJ resolution by a resolvase.

-	~~Authors~~:	+	Structural insights into sequence-dependent Holliday junction resolution by the chloroplast resolvase MOC1.,Yan J, Hong S, Guan Z, He W, Zhang D, Yin P Nat Commun. 2020 Mar 17;11(1):1417. doi: 10.1038/s41467-020-15242-8. PMID:32184398<ref>PMID:32184398</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6kvn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Guan, Z Y]]
		+	[[Category: Hong, S X]]
		+	[[Category: Yan, J J]]
		+	[[Category: Yin, P]]
		+	[[Category: Chloroplast]]
		+	[[Category: Plant protein]]
		+	[[Category: Resolvase]]

OCA: Protected "6kvn" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 06:51:16 GMT

Protected "6kvn" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:51, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kvn is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Oct 2019 06:51:14 GMT

New page: '''Unreleased structure''' The entry 6kvn is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6kvn is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]