6kww - Revision history

OCA at 10:45, 22 November 2023

OCA — Wed, 22 Nov 2023 10:45:41 GMT

←Older revision		Revision as of 10:45, 22 November 2023
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	==HslU from Staphylococcus aureus==		==HslU from Staphylococcus aureus==
-	<StructureSection load='6kww' size='340' side='right'caption='[[6kww]]' scene=''>	+	<StructureSection load='6kww' size='340' side='right'caption='[[6kww]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KWW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KWW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kww]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KWW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KWW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kww OCA], [~~http~~://pdbe.org/6kww PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kww RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kww PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kww ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kww OCA], [https://pdbe.org/6kww PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kww RCSB], [https://www.ebi.ac.uk/pdbsum/6kww PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kww ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HSLU_STAAM HSLU_STAAM] ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.[HAMAP-Rule:MF_00249]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	HslUV is a bacterial heat shock protein complex consisting of the AAA+ ATPase component HslU and the protease component HslV. HslV is a threonine (Thr) protease employing the N-terminal Thr residue in the mature protein as the catalytic residue. To date, HslUV from Gram-negative bacteria has been extensively studied. However, the mechanisms of action and activation of HslUV from Gram-positive bacteria, which have an additional N-terminal sequence before the catalytic Thr residue, remain to be revealed. In this study, we determined the crystal structures of HslV from the Gram-positive bacterium Staphylococcus aureus with and without HslU in the crystallization conditions. The structural comparison suggested that a structural transition to the symmetric form of HslV was triggered by ATP-bound HslU. More importantly, the additional N-terminal sequence was cleaved in the presence of HslU and ATP, exposing the Thr9 residue at the N-terminus and activating the ATP-dependent protease activity. Further biochemical studies demonstrated that the exposed N-terminal Thr residue is critical for catalysis with binding to the symmetric HslU hexamer. Since eukaryotic proteasomes have a similar additional N-terminal sequence, our results will improve our understanding of the common molecular mechanisms for the activation of proteasomes.
		+
		+	Cleavage-Dependent Activation of ATP-Dependent Protease HslUV from Staphylococcus aureus.,Jeong S, Ahn J, Kwon AR, Ha NC Mol Cells. 2020 Aug 31;43(8):694-704. doi: 10.14348/molcells.2020.0074. PMID:32694241<ref>PMID:32694241</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kww" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATPase 3D structures\|ATPase 3D structures]]
		+	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
	[[Category: Ha N-C]]		[[Category: Ha N-C]]
	[[Category: Jeong S]]		[[Category: Jeong S]]

OCA at 06:45, 9 December 2020

OCA — Wed, 09 Dec 2020 06:45:12 GMT

←Older revision		Revision as of 06:45, 9 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6kww ~~is ON HOLD until Paper Publication~~	+	==HslU from Staphylococcus aureus==
-		+	<StructureSection load='6kww' size='340' side='right'caption='[[6kww]]' scene=''>
-	~~Authors~~: Ha, N.-C., ~~Jeong~~, S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KWW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KWW FirstGlance]. <br>
-	~~Description: HslU from Staphylococcus aureus~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kww FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kww OCA], [http://pdbe.org/6kww PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kww RCSB], [http://www.ebi.ac.uk/pdbsum/6kww PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kww ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Ha, N.-C]]	+	__TOC__
-	[[Category: Jeong, S]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ha N-C]]
		+	[[Category: Jeong S]]

OCA at 04:48, 23 October 2019

OCA — Wed, 23 Oct 2019 04:48:16 GMT

←Older revision		Revision as of 04:48, 23 October 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kww is ON HOLD	+	The entry 6kww is ON HOLD until Paper Publication

	Authors: Ha, N.-C., Jeong, S.		Authors: Ha, N.-C., Jeong, S.

OCA: Protected "6kww" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 06:54:29 GMT

Protected "6kww" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:54, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kww is ON HOLD Authors: Ha, N.-C., Jeong, S. Description: HslU from Staphylococcus aureus Category: Unreleased Structures [[Category: Ha, N.-C...

OCA — Wed, 02 Oct 2019 06:54:27 GMT

New page: '''Unreleased structure''' The entry 6kww is ON HOLD Authors: Ha, N.-C., Jeong, S. Description: HslU from Staphylococcus aureus Category: Unreleased Structures [[Category: Ha, N.-C...

New page

'''Unreleased structure'''

The entry 6kww is ON HOLD

Authors: Ha, N.-C., Jeong, S.

Description: HslU from Staphylococcus aureus
[[Category: Unreleased Structures]]
[[Category: Ha, N.-C]]
[[Category: Jeong, S]]