6kx6 - Revision history

OCA at 10:45, 22 November 2023

OCA — Wed, 22 Nov 2023 10:45:55 GMT

←Older revision		Revision as of 10:45, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6kx6' size='340' side='right'caption='[[6kx6]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6kx6' size='340' side='right'caption='[[6kx6]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kx6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KX6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kx6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KX6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYU:~{N}-[2-(2,4-dimethylphenyl)-4,6-dihydrothieno[3,4-c]pyrazol-3-yl]-3,4-dimethyl-benzamide'>DYU</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cry1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYU:~{N}-[2-(2,4-dimethylphenyl)-4,6-dihydrothieno[3,4-c]pyrazol-3-yl]-3,4-dimethyl-benzamide'>DYU</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx6 OCA], [https://pdbe.org/6kx6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kx6 RCSB], [https://www.ebi.ac.uk/pdbsum/6kx6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx6 OCA], [https://pdbe.org/6kx6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kx6 RCSB], [https://www.ebi.ac.uk/pdbsum/6kx6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CRY1_MOUSE CRY1_MOUSE]] Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK\|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK\|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.<ref>PMID:10428031</ref> <ref>PMID:16628007</ref> <ref>PMID:16478995</ref>	+	[https://www.uniprot.org/uniprot/CRY1_MOUSE CRY1_MOUSE] Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK\|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK\|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.<ref>PMID:10428031</ref> <ref>PMID:16628007</ref> <ref>PMID:16478995</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Aikawa, Y]]	+	[[Category: Aikawa Y]]
-	[[Category: Hirota, T]]	+	[[Category: Hirota T]]
-	[[Category: Miller~~, S A]]~~	+	[[Category: Miller SA]]
-	~~[[Category: Circadian clock protein~~]]	+

OCA at 15:24, 8 June 2021

OCA — Tue, 08 Jun 2021 15:24:37 GMT

←Older revision		Revision as of 15:24, 8 June 2021
Line 3:		Line 3:
	<StructureSection load='6kx6' size='340' side='right'caption='[[6kx6]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6kx6' size='340' side='right'caption='[[6kx6]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kx6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KX6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kx6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KX6 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYU:~{N}-[2-(2,4-dimethylphenyl)-4,6-dihydrothieno[3,4-c]pyrazol-3-yl]-3,4-dimethyl-benzamide'>DYU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYU:~{N}-[2-(2,4-dimethylphenyl)-4,6-dihydrothieno[3,4-c]pyrazol-3-yl]-3,4-dimethyl-benzamide'>DYU</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cry1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cry1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx6 OCA], [~~http~~://pdbe.org/6kx6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kx6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kx6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx6 OCA], [https://pdbe.org/6kx6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kx6 RCSB], [https://www.ebi.ac.uk/pdbsum/6kx6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CRY1_MOUSE CRY1_MOUSE]] Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK\|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK\|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.<ref>PMID:10428031</ref> <ref>PMID:16628007</ref> <ref>PMID:16478995</ref>	+	[[https://www.uniprot.org/uniprot/CRY1_MOUSE CRY1_MOUSE]] Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK\|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK\|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.<ref>PMID:10428031</ref> <ref>PMID:16628007</ref> <ref>PMID:16478995</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 09:08, 20 July 2020

OCA — Mon, 20 Jul 2020 09:08:02 GMT

←Older revision		Revision as of 09:08, 20 July 2020
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6kx6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6kx6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cryptochrome 3D structures\|Cryptochrome 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:11, 15 April 2020

OCA — Wed, 15 Apr 2020 07:11:54 GMT

←Older revision		Revision as of 07:11, 15 April 2020
Line 3:		Line 3:
	<StructureSection load='6kx6' size='340' side='right'caption='[[6kx6]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6kx6' size='340' side='right'caption='[[6kx6]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kx6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6KX6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kx6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KX6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYU:~{N}-[2-(2,4-dimethylphenyl)-4,6-dihydrothieno[3,4-c]pyrazol-3-yl]-3,4-dimethyl-benzamide'>DYU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYU:~{N}-[2-(2,4-dimethylphenyl)-4,6-dihydrothieno[3,4-c]pyrazol-3-yl]-3,4-dimethyl-benzamide'>DYU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6kx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx6 OCA], [http://pdbe.org/6kx6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kx6 RCSB], [http://www.ebi.ac.uk/pdbsum/6kx6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx6 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cry1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx6 OCA], [http://pdbe.org/6kx6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kx6 RCSB], [http://www.ebi.ac.uk/pdbsum/6kx6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CRY1_MOUSE CRY1_MOUSE]] Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK\|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK\|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.<ref>PMID:10428031</ref> <ref>PMID:16628007</ref> <ref>PMID:16478995</ref>		[[http://www.uniprot.org/uniprot/CRY1_MOUSE CRY1_MOUSE]] Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK\|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK\|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.<ref>PMID:10428031</ref> <ref>PMID:16628007</ref> <ref>PMID:16478995</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CRY1 and CRY2 are essential components of the circadian clock controlling daily physiological rhythms. Accumulating evidences indicate distinct roles of these highly homologous proteins, in addition to redundant functions. Therefore, the development of isoform-selective compounds represents an effective approach towards understanding the similarities and differences of CRY1 and CRY2 by controlling each isoform individually. We conducted phenotypic screenings of circadian clock modulators, and identified KL101 and TH301 that selectively stabilize CRY1 and CRY2, respectively. Crystal structures of CRY-compound complexes revealed conservation of compound-binding sites between CRY1 and CRY2. We further discovered a unique mechanism underlying compound selectivity in which the disordered C-terminal region outside the pocket was required for the differential effects of KL101 and TH301 against CRY isoforms. By using these compounds, we found a new role of CRY1 and CRY2 as enhancers of brown adipocyte differentiation, providing the basis of CRY-mediated regulation of energy expenditure.
		+
		+	Isoform-selective regulation of mammalian cryptochromes.,Miller S, Son YL, Aikawa Y, Makino E, Nagai Y, Srivastava A, Oshima T, Sugiyama A, Hara A, Abe K, Hirata K, Oishi S, Hagihara S, Sato A, Tama F, Itami K, Kay SA, Hatori M, Hirota T Nat Chem Biol. 2020 Mar 30. pii: 10.1038/s41589-020-0505-1. doi:, 10.1038/s41589-020-0505-1. PMID:32231341<ref>PMID:32231341</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kx6" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 14:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Aikawa, Y]]		[[Category: Aikawa, Y]]
	[[Category: Hirota, T]]		[[Category: Hirota, T]]
	[[Category: Miller, S A]]		[[Category: Miller, S A]]
	[[Category: Circadian clock protein]]		[[Category: Circadian clock protein]]

OCA at 09:07, 1 April 2020

OCA — Wed, 01 Apr 2020 09:07:50 GMT

←Older revision		Revision as of 09:07, 1 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6kx6 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of mouse Cryptochrome 1 in complex with KL101 compound==
-		+	<StructureSection load='6kx6' size='340' side='right'caption='[[6kx6]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~: ~~Miller~~, S.A., ~~Aikawa~~, Y., ~~Hirota~~, T.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6kx6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KX6 FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~mouse Cryptochrome 1~~ in ~~complex~~ with ~~KL101 compound~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DYU:~{N}-[2-(2,4-dimethylphenyl)-4,6-dihydrothieno[3,4-c]pyrazol-3-yl]-3,4-dimethyl-benzamide'>DYU</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx6 OCA], [http://pdbe.org/6kx6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kx6 RCSB], [http://www.ebi.ac.uk/pdbsum/6kx6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx6 ProSAT]</span></td></tr>
-	[[Category: ~~Miller~~, ~~S.A~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CRY1_MOUSE CRY1_MOUSE]] Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK\|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK\|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.<ref>PMID:10428031</ref> <ref>PMID:16628007</ref> <ref>PMID:16478995</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Aikawa, Y]]
	[[Category: Hirota, T]]		[[Category: Hirota, T]]
-	[[Category: ~~Aikawa~~, Y]]	+	[[Category: Miller, S A]]
		+	[[Category: Circadian clock protein]]

OCA: Protected "6kx6" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 06:55:12 GMT

Protected "6kx6" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:55, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kx6 is ON HOLD until Paper Publication Authors: Miller, S.A., Aikawa, Y., Hirota, T. Description: Crystal structure of mouse Cryptochrome 1 in com...

OCA — Wed, 02 Oct 2019 06:55:10 GMT

New page: '''Unreleased structure''' The entry 6kx6 is ON HOLD until Paper Publication Authors: Miller, S.A., Aikawa, Y., Hirota, T. Description: Crystal structure of mouse Cryptochrome 1 in com...

New page

'''Unreleased structure'''

The entry 6kx6 is ON HOLD until Paper Publication

Authors: Miller, S.A., Aikawa, Y., Hirota, T.

Description: Crystal structure of mouse Cryptochrome 1 in complex with KL101 compound
[[Category: Unreleased Structures]]
[[Category: Miller, S.A]]
[[Category: Hirota, T]]
[[Category: Aikawa, Y]]