6kxb - Revision history

OCA at 10:46, 22 November 2023

OCA — Wed, 22 Nov 2023 10:46:05 GMT

←Older revision		Revision as of 10:46, 22 November 2023
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	<StructureSection load='6kxb' size='340' side='right'caption='[[6kxb]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6kxb' size='340' side='right'caption='[[6kxb]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kxb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kxb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KXB FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADA:ALPHA~~-~~D-GALACTOPYRANURONIC+ACID'>ADA</scene>~~, ~~<scene name='pdbligand=GTR:BETA-D-GALACTOPYRANURONIC+ACID'>GTR</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~LGALS3, MAC2 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=GTR:BETA-D-GALACTOPYRANURONIC+ACID'>GTR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxb OCA], [https://pdbe.org/6kxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kxb RCSB], [https://www.ebi.ac.uk/pdbsum/6kxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxb OCA], [https://pdbe.org/6kxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kxb RCSB], [https://www.ebi.ac.uk/pdbsum/6kxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LEG3_HUMAN LEG3_HUMAN]] Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.<ref>PMID:15181153</ref> <ref>PMID:19594635</ref> <ref>PMID:19616076</ref>	+	[https://www.uniprot.org/uniprot/LEG3_HUMAN LEG3_HUMAN] Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.<ref>PMID:15181153</ref> <ref>PMID:19594635</ref> <ref>PMID:19616076</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6kxb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6kxb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galectin 3D structures\|Galectin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Su, J]]	+	[[Category: Su J]]
-	~~[[Category: Gala trimer]]~~	+
-	~~[[Category: Galectin-3 crd]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 08:27, 21 April 2021

OCA — Wed, 21 Apr 2021 08:27:33 GMT

←Older revision		Revision as of 08:27, 21 April 2021
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	<StructureSection load='6kxb' size='340' side='right'caption='[[6kxb]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6kxb' size='340' side='right'caption='[[6kxb]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kxb]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kxb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KXB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=GTR:BETA-D-GALACTOPYRANURONIC+ACID'>GTR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=GTR:BETA-D-GALACTOPYRANURONIC+ACID'>GTR</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGALS3, MAC2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGALS3, MAC2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxb OCA], [~~http~~://pdbe.org/6kxb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kxb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kxb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxb OCA], [https://pdbe.org/6kxb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kxb RCSB], [https://www.ebi.ac.uk/pdbsum/6kxb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LEG3_HUMAN LEG3_HUMAN]] Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.<ref>PMID:15181153</ref> <ref>PMID:19594635</ref> <ref>PMID:19616076</ref>	+	[[https://www.uniprot.org/uniprot/LEG3_HUMAN LEG3_HUMAN]] Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.<ref>PMID:15181153</ref> <ref>PMID:19594635</ref> <ref>PMID:19616076</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:20, 30 September 2020

OCA — Wed, 30 Sep 2020 07:20:23 GMT

←Older revision		Revision as of 07:20, 30 September 2020
Line 1:		Line 1:

	==Galectin-3 CRD binds to GalA trimer==		==Galectin-3 CRD binds to GalA trimer==
-	<StructureSection load='6kxb' size='340' side='right'caption='[[6kxb]]' scene=''>	+	<StructureSection load='6kxb' size='340' side='right'caption='[[6kxb]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kxb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXB FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxb OCA], [http://pdbe.org/6kxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxb RCSB], [http://www.ebi.ac.uk/pdbsum/6kxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxb ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=GTR:BETA-D-GALACTOPYRANURONIC+ACID'>GTR</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGALS3, MAC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxb OCA], [http://pdbe.org/6kxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxb RCSB], [http://www.ebi.ac.uk/pdbsum/6kxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxb ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LEG3_HUMAN LEG3_HUMAN]] Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.<ref>PMID:15181153</ref> <ref>PMID:19594635</ref> <ref>PMID:19616076</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Galectin-3 is crucial to many physiological and pathological processes. The generally accepted dogma is that galectins function extracellularly by binding specifically to beta(1-->4)-galactoside epitopes on cell surface glycoconjugates. Here, we used crystallography and NMR spectroscopy to demonstrate that negatively charged homogalacturonans (HG, linear polysaccharides of alpha(1-->4)-linked-D-galacturonate (GalA)) bind to the galectin-3 carbohydrate recognition domain. The HG carboxylates at the C6 positions in GalA rings mandate that this saccharide bind galectin-3 in an unconventional, "topsy-turvy" orientation that is flipped by about 180o relative to that of the canonical beta-galactoside lactose. In this binding mode, the reducing end GalA beta-anomer of HGs takes the position of the nonreducing end galactose residue in lactose. This novel orientation maintains interactions with the conserved tryptophan and seven of the most crucial lactose-binding residues, albeit with different H-bonding interactions. Nevertheless, the HG molecular orientation and new interactions have essentially the same thermodynamic binding parameters as lactose. Overall, our study provides structural details for a new type of galectin-sugar interaction that broadens glycospace for ligand binding to Gal-3 and suggests how the lectin may recognize other negatively charged polysaccharides like glycoaminoglycans (e.g. heparan sulfate) on the cell surface. This discovery impacts on our understanding of galectin-mediated biological function.
		+
		+	Topsy-turvy binding of negatively charged homogalacturonan oligosaccharides to galectin-3.,Zheng Y, Su J, Miller MC, Geng J, Xu X, Zhang T, Mayzel M, Zhou Y, Mayo KH, Tai G Glycobiology. 2020 Aug 17. pii: 5892985. doi: 10.1093/glycob/cwaa080. PMID:32909036<ref>PMID:32909036</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kxb" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Su J]]	+	[[Category: Su, J]]
		+	[[Category: Gala trimer]]
		+	[[Category: Galectin-3 crd]]
		+	[[Category: Sugar binding protein]]

OCA at 11:11, 26 August 2020

OCA — Wed, 26 Aug 2020 11:11:24 GMT

←Older revision		Revision as of 11:11, 26 August 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6kxb is ON HOLD until Paper Publication~~	+	==Galectin-3 CRD binds to GalA trimer==
-		+	<StructureSection load='6kxb' size='340' side='right'caption='[[6kxb]]' scene=''>
-	~~Authors: Su, J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXB FirstGlance]. <br>
-	~~Description:~~ Galectin-3 CRD binds to GalA trimer	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxb OCA], [http://pdbe.org/6kxb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxb RCSB], [http://www.ebi.ac.uk/pdbsum/6kxb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxb ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Su, J]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Su J]]

OCA at 09:17, 29 July 2020

OCA — Wed, 29 Jul 2020 09:17:50 GMT

←Older revision		Revision as of 09:17, 29 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kxb is ON HOLD	+	The entry 6kxb is ON HOLD until Paper Publication

	Authors: Su, J.		Authors: Su, J.

OCA at 05:47, 22 July 2020

OCA — Wed, 22 Jul 2020 05:47:36 GMT

←Older revision		Revision as of 05:47, 22 July 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kxb is ON HOLD ~~until Paper Publication~~	+	The entry 6kxb is ON HOLD

	Authors: Su, J.		Authors: Su, J.

OCA at 07:17, 30 October 2019

OCA — Wed, 30 Oct 2019 07:17:18 GMT

←Older revision		Revision as of 07:17, 30 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kxb is ON HOLD	+	The entry 6kxb is ON HOLD until Paper Publication

	Authors: Su, J.		Authors: Su, J.

OCA: Protected "6kxb" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 06:55:39 GMT

Protected "6kxb" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:55, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kxb is ON HOLD Authors: Su, J. Description: Galectin-3 CRD binds to GalA trimer Category: Unreleased Structures Category: Su, J

OCA — Wed, 02 Oct 2019 06:55:37 GMT

New page: '''Unreleased structure''' The entry 6kxb is ON HOLD Authors: Su, J. Description: Galectin-3 CRD binds to GalA trimer Category: Unreleased Structures Category: Su, J

New page

'''Unreleased structure'''

The entry 6kxb is ON HOLD

Authors: Su, J.

Description: Galectin-3 CRD binds to GalA trimer
[[Category: Unreleased Structures]]
[[Category: Su, J]]