6kxf - Revision history

OCA at 14:30, 8 July 2020

OCA — Wed, 08 Jul 2020 14:30:42 GMT

←Older revision		Revision as of 14:30, 8 July 2020
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	<StructureSection load='6kxf' size='340' side='right'caption='[[6kxf]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='6kxf' size='340' side='right'caption='[[6kxf]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kxf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kxf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._msc090213je08 Streptomyces sp. msc090213je08]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYF:[(3~{R})-2,2-dimethyl-4-[[3-[2-[[(~{E})-oct-2-enoyl]amino]ethylamino]-3-oxidanylidene-propyl]amino]-3-oxidanyl-4-oxidanylidene-butyl]+dihydrogen+phosphate'>DYF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYF:[(3~{R})-2,2-dimethyl-4-[[3-[2-[[(~{E})-oct-2-enoyl]amino]ethylamino]-3-oxidanylidene-propyl]amino]-3-oxidanyl-4-oxidanylidene-butyl]+dihydrogen+phosphate'>DYF</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">iga11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1670457 Streptomyces sp. MSC090213JE08]), iga12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1670457 Streptomyces sp. MSC090213JE08]), iga10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1670457 Streptomyces sp. MSC090213JE08])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxf OCA], [http://pdbe.org/6kxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxf RCSB], [http://www.ebi.ac.uk/pdbsum/6kxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxf OCA], [http://pdbe.org/6kxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxf RCSB], [http://www.ebi.ac.uk/pdbsum/6kxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxf ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptomyces sp. msc090213je08]]
	[[Category: Burkart, M]]		[[Category: Burkart, M]]
	[[Category: Chen, A]]		[[Category: Chen, A]]

OCA at 06:00, 20 May 2020

OCA — Wed, 20 May 2020 06:00:14 GMT

←Older revision		Revision as of 06:00, 20 May 2020
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	==The ishigamide ketosynthase/chain length factor==		==The ishigamide ketosynthase/chain length factor==
-	<StructureSection load='6kxf' size='340' side='right'caption='[[6kxf]]' scene=''>	+	<StructureSection load='6kxf' size='340' side='right'caption='[[6kxf]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kxf]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxf OCA], [http://pdbe.org/6kxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxf RCSB], [http://www.ebi.ac.uk/pdbsum/6kxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxf ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DYF:[(3~{R})-2,2-dimethyl-4-[[3-[2-[[(~{E})-oct-2-enoyl]amino]ethylamino]-3-oxidanylidene-propyl]amino]-3-oxidanyl-4-oxidanylidene-butyl]+dihydrogen+phosphate'>DYF</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxf OCA], [http://pdbe.org/6kxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxf RCSB], [http://www.ebi.ac.uk/pdbsum/6kxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxf ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In type II polyketide synthases (PKSs), the ketosynthase-chain length factor (KS-CLF) complex catalyzes polyketide chain elongation with the acyl carrier protein (ACP). Highly reducing type II PKSs, represented by IgaPKS, produce polyene structures instead of the well-known aromatic skeletons. Here, we report the crystal structures of the Iga11-Iga12 (KS-CLF) heterodimer and the covalently cross-linked Iga10=Iga11-Iga12 (ACP=KS-CLF) tripartite complex. The latter structure revealed the molecular basis of the interaction between Iga10 and Iga11-Iga12, which differs from that between the ACP and KS of Escherichia coli fatty acid synthase. Furthermore, the reaction pocket structure and site-directed mutagenesis revealed that the negative charge of Asp 113 of Iga11 prevents further condensation using a beta-ketoacyl product as a substrate, which distinguishes IgaPKS from typical type II PKSs. This work will facilitate the future rational design of PKSs.
		+
		+	Structural basis for selectivity in a highly reducing type II polyketide synthase.,Du D, Katsuyama Y, Horiuchi M, Fushinobu S, Chen A, Davis TD, Burkart MD, Ohnishi Y Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0530-0. doi:, 10.1038/s41589-020-0530-0. PMID:32367018<ref>PMID:32367018</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kxf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Burkart M]]	+	[[Category: Burkart, M]]
-	[[Category: Chen A]]	+	[[Category: Chen, A]]
-	[[Category: Davis T]]	+	[[Category: Davis, T]]
-	[[Category: Du D]]	+	[[Category: Du, D]]
-	[[Category: Fushinobu S]]	+	[[Category: Fushinobu, S]]
-	[[Category: Horiuchi M]]	+	[[Category: Horiuchi, M]]
-	[[Category: Katsuyama Y]]	+	[[Category: Katsuyama, Y]]
-	[[Category: Ohnishi Y]]	+	[[Category: Ohnishi, Y]]
		+	[[Category: Polyketide synthase]]
		+	[[Category: Transferase]]

OCA at 05:59, 6 May 2020

OCA — Wed, 06 May 2020 05:59:14 GMT

←Older revision		Revision as of 05:59, 6 May 2020
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-	'''Unreleased structure'''

-	The ~~entry~~ 6kxf is ~~ON HOLD until Sep 10 2021~~	+	==The ishigamide ketosynthase/chain length factor==
-		+	<StructureSection load='6kxf' size='340' side='right'caption='[[6kxf]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KXF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KXF FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kxf OCA], [http://pdbe.org/6kxf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kxf RCSB], [http://www.ebi.ac.uk/pdbsum/6kxf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kxf ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Burkart M]]
		+	[[Category: Chen A]]
		+	[[Category: Davis T]]
		+	[[Category: Du D]]
		+	[[Category: Fushinobu S]]
		+	[[Category: Horiuchi M]]
		+	[[Category: Katsuyama Y]]
		+	[[Category: Ohnishi Y]]

OCA at 07:17, 30 October 2019

OCA — Wed, 30 Oct 2019 07:17:28 GMT

←Older revision		Revision as of 07:17, 30 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kxf is ON HOLD	+	The entry 6kxf is ON HOLD until Sep 10 2021

	Authors:		Authors:

OCA: Protected "6kxf" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 06:55:54 GMT

Protected "6kxf" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:55, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kxf is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Oct 2019 06:55:53 GMT

New page: '''Unreleased structure''' The entry 6kxf is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6kxf is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]