6kys - Revision history

OCA at 10:47, 22 November 2023

OCA — Wed, 22 Nov 2023 10:47:19 GMT

←Older revision		Revision as of 10:47, 22 November 2023
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	==The structure of the M. tb toxin MazF-mt1==		==The structure of the M. tb toxin MazF-mt1==
-	<StructureSection load='6kys' size='340' side='right'caption='[[6kys]]' scene=''>	+	<StructureSection load='6kys' size='340' side='right'caption='[[6kys]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KYS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6KYS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kys]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KYS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KYS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6kys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kys OCA], [~~http~~://pdbe.org/6kys PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kys RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kys PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kys ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.200414Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kys OCA], [https://pdbe.org/6kys PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kys RCSB], [https://www.ebi.ac.uk/pdbsum/6kys PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kys ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MAZF9_MYCTU MAZF9_MYCTU] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE9. Acts as an mRNA interferase, specifically cleaving between U and C in UAC sequences. May cleave its cognate antitoxin's gene (PubMed:25608501). In E.coli expression with non-cognate antitoxins VapB27 and VapB40 partially neutralizes the toxin.<ref>PMID:16611633</ref> <ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:20876537</ref> <ref>PMID:25608501</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Toxin-antitoxin (TA) systems, which regulate many important cellular processes, are abundantly present in prokaryotic organisms. MazEF is a common type of TA system implicated in the formation of "persisters cells" of the pathogen Mycobacterium tuberculosis, which contains 10 such systems. However, the exact function and inhibition mode of each MazF protein are not quite understood. Here, we report four high-resolution crystal structures of MazF-mt1 in various forms, including one in complex with MazE-mt1. The toxin displayed two unique interlocked loops that allow the formation of a tight dimer. These loops would open upon interacting with the MazE-mt1 antitoxin mediated by the last two helices of MazE-mt1. With our structure-based design, a mutant that could bind to the antitoxin with an enhanced affinity was produced. Combined crystallographic and biochemical studies further revealed that the binding affinity of MazE-mt1 to MazF-mt1 was mainly attributed to its alpha3 helical region, while the terminal helix eta1 contributes very little or even negatively to the association of the pair, in stark contrast to the MazEF-mt9 system. This study provides structural insight into the binding mode and the inhibition mechanism of the MazE/F-mt1 TA pair, which may reflect the functional differences between different TA systems.
		+
		+	Conserved Conformational Changes in the Regulation of Mycobacterium tuberculosis MazEF-mt1.,Chen R, Zhou J, Sun R, Du C, Xie W ACS Infect Dis. 2020 Jul 10;6(7):1783-1795. doi: 10.1021/acsinfecdis.0c00048., Epub 2020 Jun 16. PMID:32485099<ref>PMID:32485099</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kys" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Mycobacterium tuberculosis H37Rv]]
	[[Category: Chen R]]		[[Category: Chen R]]
	[[Category: Xie W]]		[[Category: Xie W]]
	[[Category: Zhou J]]		[[Category: Zhou J]]

OCA at 05:31, 5 August 2020

OCA — Wed, 05 Aug 2020 05:31:49 GMT

←Older revision		Revision as of 05:31, 5 August 2020
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-	'''Unreleased structure'''

-	The ~~entry~~ 6kys ~~is ON HOLD until Sep 20 2021~~	+	==The structure of the M. tb toxin MazF-mt1==
-		+	<StructureSection load='6kys' size='340' side='right'caption='[[6kys]]' scene=''>
-	~~Authors~~: ~~Xie~~, W., ~~Chen, R~~., ~~Zhou~~, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KYS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KYS FirstGlance]. <br>
-	~~Description~~: ~~The structure of the M~~. ~~tb toxin MazF~~-~~mt1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kys FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kys OCA], [http://pdbe.org/6kys PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kys RCSB], [http://www.ebi.ac.uk/pdbsum/6kys PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kys ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Xie, W~~]]	+	__TOC__
-	[[Category: ~~Chen, R~~]]	+	</StructureSection>
-	[[Category: Zhou, J]]	+	[[Category: Large Structures]]
		+	[[Category: Chen R]]
		+	[[Category: Xie W]]
		+	[[Category: Zhou J]]

OCA at 03:33, 10 October 2019

OCA — Thu, 10 Oct 2019 03:33:15 GMT

←Older revision		Revision as of 03:33, 10 October 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kys is ON HOLD	+	The entry 6kys is ON HOLD until Sep 20 2021

	Authors: Xie, W., Chen, R., Zhou, J.		Authors: Xie, W., Chen, R., Zhou, J.

OCA: Protected "6kys" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 07:00:36 GMT

Protected "6kys" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:00, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kys is ON HOLD Authors: Xie, W., Chen, R., Zhou, J. Description: The structure of the M. tb toxin MazF-mt1 Category: Unreleased Structures [[C...

OCA — Wed, 02 Oct 2019 07:00:34 GMT

New page: '''Unreleased structure''' The entry 6kys is ON HOLD Authors: Xie, W., Chen, R., Zhou, J. Description: The structure of the M. tb toxin MazF-mt1 Category: Unreleased Structures [[C...

New page

'''Unreleased structure'''

The entry 6kys is ON HOLD

Authors: Xie, W., Chen, R., Zhou, J.

Description: The structure of the M. tb toxin MazF-mt1
[[Category: Unreleased Structures]]
[[Category: Xie, W]]
[[Category: Chen, R]]
[[Category: Zhou, J]]