OCA at 10:47, 22 November 2023

OCA — Wed, 22 Nov 2023 10:47:35 GMT

←Older revision		Revision as of 10:47, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6kzn' size='340' side='right'caption='[[6kzn]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6kzn' size='340' side='right'caption='[[6kzn]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6kzn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KZN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6KZN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6kzn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KZN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KZN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E1C:2,5-diethyl-1-methyl-4-sulfamoyl-pyrrole-3-carboxylic+acid'>E1C</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6kzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kzn OCA], [~~http~~://pdbe.org/6kzn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6kzn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6kzn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6kzn ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E1C:2,5-diethyl-1-methyl-4-sulfamoyl-pyrrole-3-carboxylic+acid'>E1C</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kzn OCA], [https://pdbe.org/6kzn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kzn RCSB], [https://www.ebi.ac.uk/pdbsum/6kzn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kzn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9K2N0_PSEAI Q9K2N0_PSEAI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Production of metallo-beta-lactamases (MBLs), which hydrolyze carbapenems, is a cause of carbapenem resistance in Enterobacteriaceae Development of effective inhibitors for MBLs is one approach to restore carbapenem efficacy in carbapenem-resistant Enterobacteriaceae (CRE). We report here that sulfamoyl heteroarylcarboxylic acids (SHCs) can competitively inhibit the globally spreading and clinically relevant MBLs (i.e., IMP-, NDM-, and VIM-type MBLs) at nanomolar to micromolar orders of magnitude. Addition of SHCs restored meropenem efficacy against 17/19 IMP-type and 7/14 NDM-type MBL-producing Enterobacteriaceae to satisfactory clinical levels. SHCs were also effective against IMP-type MBL-producing Acinetobacter spp. and engineered Escherichia coli strains overproducing individual minor MBLs (i.e., TMB-2, SPM-1, DIM-1, SIM-1, and KHM-1). However, SHCs were less effective against MBL-producing Pseudomonas aeruginosa Combination therapy with meropenem and SHCs successfully cured mice infected with IMP-1-producing E. coli and dually NDM-1/VIM-1-producing Klebsiella pneumoniae clinical isolates. X-ray crystallographic analyses revealed the inhibition mode of SHCs against MBLs; the sulfamoyl group of SHCs coordinated to two zinc ions, and the carboxylate group coordinated to one zinc ion and bound to positively charged amino acids Lys224/Arg228 conserved in MBLs. Preclinical testing revealed that the SHCs showed low toxicity in cell lines and mice and high stability in human liver microsomes. Our results indicate that SHCs are promising lead compounds for inhibitors of MBLs to combat MBL-producing CRE.IMPORTANCE Carbapenem antibiotics are the last resort for control of severe infectious diseases, bloodstream infections, and pneumonia caused by Gram-negative bacteria, including Enterobacteriaceae However, carbapenem-resistant Enterobacteriaceae (CRE) strains have spread globally and are a critical concern in clinical settings because CRE infections are recognized as a leading cause of increased mortality among hospitalized patients. Most CRE produce certain kinds of serine carbapenemases (e.g., KPC- and GES-type beta-lactamases) or metallo-beta-lactamases (MBLs), which can hydrolyze carbapenems. Although effective MBL inhibitors are expected to restore carbapenem efficacy against MBL-producing CRE, no MBL inhibitor is currently clinically available. Here, we synthesized 2,5-diethyl-1-methyl-4-sulfamoylpyrrole-3-carboxylic acid (SPC), which is a potent inhibitor of MBLs. SPC is a remarkable lead compound for clinically useful MBL inhibitors and can potentially provide a considerable benefit to patients receiving treatment for lethal infectious diseases caused by MBL-producing CRE.
		+
		+	Sulfamoyl Heteroarylcarboxylic Acids as Promising Metallo-beta-Lactamase Inhibitors for Controlling Bacterial Carbapenem Resistance.,Wachino JI, Jin W, Kimura K, Kurosaki H, Sato A, Arakawa Y mBio. 2020 Mar 17;11(2):e03144-19. doi: 10.1128/mBio.03144-19. PMID:32184250<ref>PMID:32184250</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6kzn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Wachino, J~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Hydrolase~~]]	+	[[Category: Wachino J]]

OCA at 06:21, 19 February 2020

OCA — Wed, 19 Feb 2020 06:21:12 GMT

←Older revision		Revision as of 06:21, 19 February 2020
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-	'''Unreleased structure'''

-	~~The entry 6kzn is ON HOLD until Sep 24 2021~~	+	==Crystal Structure Of VIM-2 Metallo-beta-lactamase In Complex With Inhibitor X2==
-		+	<StructureSection load='6kzn' size='340' side='right'caption='[[6kzn]], [[Resolution\|resolution]] 1.50Å' scene=''>
-	~~Authors: Wachino, J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6kzn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KZN FirstGlance]. <br>
-	~~Description:~~ Crystal Structure Of VIM-2 Metallo-beta-lactamase In Complex With Inhibitor X2	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=E1C:2,5-diethyl-1-methyl-4-sulfamoyl-pyrrole-3-carboxylic+acid'>E1C</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kzn OCA], [http://pdbe.org/6kzn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kzn RCSB], [http://www.ebi.ac.uk/pdbsum/6kzn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kzn ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Wachino, J]]		[[Category: Wachino, J]]
		+	[[Category: Hydrolase]]

OCA at 07:17, 30 October 2019

OCA — Wed, 30 Oct 2019 07:17:54 GMT

←Older revision		Revision as of 07:17, 30 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6kzn is ON HOLD	+	The entry 6kzn is ON HOLD until Sep 24 2021

	Authors: Wachino, J.		Authors: Wachino, J.

OCA: Protected "6kzn" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 07:03:02 GMT

Protected "6kzn" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:03, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6kzn is ON HOLD Authors: Wachino, J. Description: Crystal Structure Of VIM-2 Metallo-beta-lactamase In Complex With Inhibitor X2 [[Category: Unrele...

OCA — Wed, 02 Oct 2019 07:03:00 GMT

New page: '''Unreleased structure''' The entry 6kzn is ON HOLD Authors: Wachino, J. Description: Crystal Structure Of VIM-2 Metallo-beta-lactamase In Complex With Inhibitor X2 [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 6kzn is ON HOLD

Authors: Wachino, J.

Description: Crystal Structure Of VIM-2 Metallo-beta-lactamase In Complex With Inhibitor X2
[[Category: Unreleased Structures]]
[[Category: Wachino, J]]

6kzn - Revision history

OCA at 10:47, 22 November 2023

OCA at 06:21, 19 February 2020

OCA at 07:17, 30 October 2019

OCA: Protected "6kzn" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6kzn is ON HOLD Authors: Wachino, J. Description: Crystal Structure Of VIM-2 Metallo-beta-lactamase In Complex With Inhibitor X2 [[Category: Unrele...