6l0g - Revision history

OCA at 10:48, 22 November 2023

OCA — Wed, 22 Nov 2023 10:48:01 GMT

←Older revision		Revision as of 10:48, 22 November 2023
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	<StructureSection load='6l0g' size='340' side='right'caption='[[6l0g]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='6l0g' size='340' side='right'caption='[[6l0g]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6l0g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l0g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L0G FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=LMR:(2S)~~-2~~-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.053Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;~~'>~~[[6l0a\|6l0a]], [[6l0b\|6l0b]], [[6l0f\|6l0f]]~~</~~div~~>~~</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">URA4~~, ~~YLR420W, L9931.1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Dihydroorotase Dihydroorotase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.3 3.5.2.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0g OCA], [https://pdbe.org/6l0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l0g RCSB], [https://www.ebi.ac.uk/pdbsum/6l0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0g OCA], [https://pdbe.org/6l0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l0g RCSB], [https://www.ebi.ac.uk/pdbsum/6l0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0g ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PYRC_YEAST PYRC_YEAST]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6l0g" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6l0g" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dihydroorotase 3D structures\|Dihydroorotase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
-	[[Category: Dihydroorotase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chen, C J~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Guan, H H~~]]	+	[[Category: Chen CJ]]
-	[[Category: ~~Huang, C Y~~]]	+	[[Category: Guan HH]]
-	[[Category: Huang~~, Y H~~]]	+	[[Category: Huang CY]]
-	[[Category: ~~Dihydropyrimidinase dihydroorotase metalloenzyme]]~~	+	[[Category: Huang YH]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 14:50, 17 June 2021

OCA — Thu, 17 Jun 2021 14:50:33 GMT

←Older revision		Revision as of 14:50, 17 June 2021
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	==Crystal structure of dihydroorotase in complex with malate at pH6 from Saccharomyces cerevisiae==		==Crystal structure of dihydroorotase in complex with malate at pH6 from Saccharomyces cerevisiae==
-	<StructureSection load='6l0g' size='340' side='right'caption='[[6l0g]]' scene=''>	+	<StructureSection load='6l0g' size='340' side='right'caption='[[6l0g]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6L0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l0g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L0G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6l0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0g OCA], [~~http~~://pdbe.org/6l0g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6l0g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6l0g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0g ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6l0a\|6l0a]], [[6l0b\|6l0b]], [[6l0f\|6l0f]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">URA4, YLR420W, L9931.1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Dihydroorotase Dihydroorotase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.3 3.5.2.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0g OCA], [https://pdbe.org/6l0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l0g RCSB], [https://www.ebi.ac.uk/pdbsum/6l0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0g ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dihydroorotase (DHOase) is the third enzyme in the de novo biosynthesis pathway of pyrimidine nucleotides and considered an attractive target for potential antimalarial, anticancer, and antipathogen chemotherapy. Whether the FDA-approved clinical drug 5-fluorouracil (5-FU) that is used to target the enzyme thymidylate synthase for anticancer therapy can also bind to DHOase remains unknown. Here, we report the crystal structures of DHOase from Saccharomyces cerevisiae (ScDHOase) complexed with malate, 5-FU, and 5-aminouracil (5-AU). ScDHOase shares structural similarity with Escherichia coli DHOase. We also characterized the binding of 5-FU and 5-AU to ScDHOase by using the fluorescence quenching method. These complexed structures revealed that residues Arg18, Asn43, Thr106, and Ala275 of ScDHOase were involved in the 5-FU (PDB entry 6L0B) and 5-AU binding (PDB entry 6L0F). Overall, these results provide structural insights that may facilitate the development of new inhibitors targeting DHOase and constitute the 5-FU and 5-AU interactomes for further clinical chemotherapies.
		+
		+	Structural basis for the interaction modes of dihydroorotase with the anticancer drugs 5-fluorouracil and 5-aminouracil.,Guan HH, Huang YH, Lin ES, Chen CJ, Huang CY Biochem Biophys Res Commun. 2021 Apr 30;551:33-37. doi:, 10.1016/j.bbrc.2021.03.001. Epub 2021 Mar 11. PMID:33714757<ref>PMID:33714757</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6l0g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
		+	[[Category: Dihydroorotase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen CJ]]	+	[[Category: Chen, C J]]
-	[[Category: Guan HH]]	+	[[Category: Guan, H H]]
-	[[Category: Huang CY]]	+	[[Category: Huang, C Y]]
-	[[Category: Huang YH]]	+	[[Category: Huang, Y H]]
		+	[[Category: Dihydropyrimidinase dihydroorotase metalloenzyme]]
		+	[[Category: Hydrolase]]

OCA at 07:42, 2 December 2020

OCA — Wed, 02 Dec 2020 07:42:32 GMT

←Older revision		Revision as of 07:42, 2 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6l0g ~~is ON HOLD until Dec 02 2021~~	+	==Crystal structure of dihydroorotase in complex with malate at pH6 from Saccharomyces cerevisiae==
-		+	<StructureSection load='6l0g' size='340' side='right'caption='[[6l0g]]' scene=''>
-	~~Authors~~: ~~Guan~~, H.H., ~~Huang~~, Y.H., ~~Huang, C~~.Y., ~~Chen, C~~.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L0G FirstGlance]. <br>
-	~~Description: Crystal structure of dihydroorotase in complex with malate at pH6 from Saccharomyces cerevisiae~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0g OCA], [http://pdbe.org/6l0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l0g RCSB], [http://www.ebi.ac.uk/pdbsum/6l0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0g ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Huang, Y.H~~]]	+	__TOC__
-	[[Category: Guan~~, H.H~~]]	+	</StructureSection>
-	[[Category: ~~Chen, C.J~~]]	+	[[Category: Large Structures]]
-	[[Category: Huang~~, C.Y~~]]	+	[[Category: Chen CJ]]
		+	[[Category: Guan HH]]
		+	[[Category: Huang CY]]
		+	[[Category: Huang YH]]

OCA at 05:06, 25 November 2020

OCA — Wed, 25 Nov 2020 05:06:40 GMT

←Older revision		Revision as of 05:06, 25 November 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6l0g is ON HOLD until ~~Oct 31~~ 2021	+	The entry 6l0g is ON HOLD until Dec 02 2021

	Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.		Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.
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	Description: Crystal structure of dihydroorotase in complex with malate at pH6 from Saccharomyces cerevisiae		Description: Crystal structure of dihydroorotase in complex with malate at pH6 from Saccharomyces cerevisiae
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Guan, H.H]]
-	[[Category: Huang, C.Y]]
	[[Category: Huang, Y.H]]		[[Category: Huang, Y.H]]
		+	[[Category: Guan, H.H]]
	[[Category: Chen, C.J]]		[[Category: Chen, C.J]]
		+	[[Category: Huang, C.Y]]

OCA at 05:14, 4 November 2020

OCA — Wed, 04 Nov 2020 05:14:31 GMT

←Older revision		Revision as of 05:14, 4 November 2020
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	Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.		Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.

-	Description: Crystal structure of dihydroorotase in complex with malate at pH6 from ~~Staphylococcus aureus~~	+	Description: Crystal structure of dihydroorotase in complex with malate at pH6 from Saccharomyces cerevisiae
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Huang, Y.H]]
	[[Category: Guan, H.H]]		[[Category: Guan, H.H]]
-	[[Category: Chen, C.J]]
	[[Category: Huang, C.Y]]		[[Category: Huang, C.Y]]
		+	[[Category: Huang, Y.H]]
		+	[[Category: Chen, C.J]]

OCA at 09:02, 26 August 2020

OCA — Wed, 26 Aug 2020 09:02:12 GMT

←Older revision		Revision as of 09:02, 26 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6l0g is ON HOLD until ~~Paper Publication~~	+	The entry 6l0g is ON HOLD until Oct 31 2021

	Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.		Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.
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	[[Category: Huang, Y.H]]		[[Category: Huang, Y.H]]
	[[Category: Guan, H.H]]		[[Category: Guan, H.H]]
-	[[Category: Huang, C.Y]]
	[[Category: Chen, C.J]]		[[Category: Chen, C.J]]
		+	[[Category: Huang, C.Y]]

OCA at 06:30, 1 January 2020

OCA — Wed, 01 Jan 2020 06:30:37 GMT

←Older revision		Revision as of 06:30, 1 January 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6l0g is ON HOLD	+	The entry 6l0g is ON HOLD until Paper Publication

	Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.		Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.
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	[[Category: Huang, Y.H]]		[[Category: Huang, Y.H]]
	[[Category: Guan, H.H]]		[[Category: Guan, H.H]]
-	[[Category: Chen, C.J]]
	[[Category: Huang, C.Y]]		[[Category: Huang, C.Y]]
		+	[[Category: Chen, C.J]]

OCA at 07:03, 25 December 2019

OCA — Wed, 25 Dec 2019 07:03:22 GMT

←Older revision		Revision as of 07:03, 25 December 2019
Line 3:		Line 3:
	The entry 6l0g is ON HOLD		The entry 6l0g is ON HOLD

-	Authors: Guan, H.H., Huang, Y.H., Huang, C.H., Chen, C.J.	+	Authors: Guan, H.H., Huang, Y.H., Huang, C.Y., Chen, C.J.

-	Description: Crystal structure of dihydroorotase from Staphylococcus aureus	+	Description: Crystal structure of dihydroorotase in complex with malate at pH6 from Staphylococcus aureus
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Huang, C.H]]
-	[[Category: Guan, H.H]]
	[[Category: Huang, Y.H]]		[[Category: Huang, Y.H]]
		+	[[Category: Guan, H.H]]
	[[Category: Chen, C.J]]		[[Category: Chen, C.J]]
		+	[[Category: Huang, C.Y]]

OCA: Protected "6l0g" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 07:05:50 GMT

Protected "6l0g" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:05, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6l0g is ON HOLD Authors: Guan, H.H., Huang, Y.H., Huang, C.H., Chen, C.J. Description: Crystal structure of dihydroorotase from Staphylococcus aure...

OCA — Wed, 02 Oct 2019 07:05:48 GMT

New page: '''Unreleased structure''' The entry 6l0g is ON HOLD Authors: Guan, H.H., Huang, Y.H., Huang, C.H., Chen, C.J. Description: Crystal structure of dihydroorotase from Staphylococcus aure...

New page

'''Unreleased structure'''

The entry 6l0g is ON HOLD

Authors: Guan, H.H., Huang, Y.H., Huang, C.H., Chen, C.J.

Description: Crystal structure of dihydroorotase from Staphylococcus aureus
[[Category: Unreleased Structures]]
[[Category: Huang, C.H]]
[[Category: Guan, H.H]]
[[Category: Huang, Y.H]]
[[Category: Chen, C.J]]