6l0r - Revision history

OCA at 10:48, 22 November 2023

OCA — Wed, 22 Nov 2023 10:48:09 GMT

←Older revision		Revision as of 10:48, 22 November 2023
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	<StructureSection load='6l0r' size='340' side='right'caption='[[6l0r]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='6l0r' size='340' side='right'caption='[[6l0r]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6l0r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aerpe Aerpe~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L0R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l0r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix_K1 Aeropyrum pernix K1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L0R FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E1R:(2S)-3-acetyloxy-2-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]propanoic+acid'>E1R</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.79Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cysO, APE_1586 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272557 AERPE])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E1R:(2S)-3-acetyloxy-2-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]propanoic+acid'>E1R</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0r OCA], [https://pdbe.org/6l0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l0r RCSB], [https://www.ebi.ac.uk/pdbsum/6l0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0r ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0r OCA], [https://pdbe.org/6l0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l0r RCSB], [https://www.ebi.ac.uk/pdbsum/6l0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0r ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CYSO_AERPE CYSO_AERPE]] Cysteine synthase that can also catalyze the synthesis of S-sulfo-L-cysteine from thiosulfate and O(3)-acetyl-L-serine, as well as the sulfhydrylation of L-serine by sulfide.<ref>PMID:12644499</ref>	+	[https://www.uniprot.org/uniprot/CYSO_AERPE CYSO_AERPE] Cysteine synthase that can also catalyze the synthesis of S-sulfo-L-cysteine from thiosulfate and O(3)-acetyl-L-serine, as well as the sulfhydrylation of L-serine by sulfide.<ref>PMID:12644499</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aerpe~~]]	+	[[Category: Aeropyrum pernix K1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ishikawa, K]]	+	[[Category: Ishikawa K]]
-	[[Category: Nakabayashi, M]]	+	[[Category: Nakabayashi M]]
-	[[Category: Nakamura, T]]	+	[[Category: Nakamura T]]
-	[[Category: Takeda, E]]	+	[[Category: Takeda E]]
-	~~[[Category: Cysteine biosynthesis]]~~	+
-	~~[[Category: Sulfhydrylase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 14:53, 3 March 2021

OCA — Wed, 03 Mar 2021 14:53:08 GMT

←Older revision		Revision as of 14:53, 3 March 2021
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	==Crystal Structure of the O-Phosphoserine Sulfhydrylase from Aeropyrum pernix Complexed with O-Acetylserine==		==Crystal Structure of the O-Phosphoserine Sulfhydrylase from Aeropyrum pernix Complexed with O-Acetylserine==
-	<StructureSection load='6l0r' size='340' side='right'caption='[[6l0r]]' scene=''>	+	<StructureSection load='6l0r' size='340' side='right'caption='[[6l0r]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0R OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6L0R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l0r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aerpe Aerpe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L0R FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6l0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0r OCA], [~~http~~://pdbe.org/6l0r PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6l0r RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6l0r PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0r ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=E1R:(2S)-3-acetyloxy-2-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]propanoic+acid'>E1R</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cysO, APE_1586 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272557 AERPE])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0r OCA], [https://pdbe.org/6l0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l0r RCSB], [https://www.ebi.ac.uk/pdbsum/6l0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0r ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/CYSO_AERPE CYSO_AERPE]] Cysteine synthase that can also catalyze the synthesis of S-sulfo-L-cysteine from thiosulfate and O(3)-acetyl-L-serine, as well as the sulfhydrylation of L-serine by sulfide.<ref>PMID:12644499</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pyridoxal-5'-phosphate-dependent cysteine synthases synthesize l-cysteine from their primary substrates, O-acetyl-l-serine (OAS) and O-phospho-l-serine (OPS), and their secondary substrate, sulfide. The mechanism by which cysteine synthases recognize OPS remains unclear; hence, we investigated the OPS recognition mechanism of the OPS sulfhydrylase obtained from Aeropyrum pernix K1 (ApOPSS) and the OAS sulfhydrylase-B obtained from Escherichia coli (EcOASS-B), using protein engineering methods. From the amino acid sequence alignment data, we found that some OPS sulfhydrylases (OPSSs) had a Tyr corresponding to the Phe225 and Phe141 residues in ApOPSS and EcOASS-B, respectively, and that the Tyr residue could facilitate OPS recognition. The enzymatic activity of the ApOPSS F225Y mutant toward OPS decreased compared with that of the wild-type; the kcat value decreased 2.3-fold during cysteine synthesis. X-ray crystallography results of the complex of ApOPSS F225Y and F225Y/R297A mutants bound to OPS and l-cysteine showed that kcat might have decreased because of the stronger interactions of the reaction product phosphate with Tyr225, Thr203, and Arg297, and that of the l-cysteine with Tyr225. The specific activity of the EcOASS-B F141Y mutant toward OPS increased by 50-fold compared with that of the wild-type. Thus, a Tyr within a cysteine synthase corresponding to the Phe225 in ApOPSS and Phe141 in EcOASS-B could act as a key residue for classifying an unknown cysteine synthase as an OPSS. The elucidation of the substrate recognition system of cysteine synthases would enable us to effectively classify cysteine synthases and develop pathogen-specific drug targets, as OPSS is absent in mammalian hosts.
		+
		+	Identification of amino acid residues important for recognition of O-phospho-l-serine substrates by cysteine synthase.,Takeda E, Matsui E, Kiryu T, Nakagawa T, Nakabayashi M, Ishikawa K, Nakamura T J Biosci Bioeng. 2021 Feb 6. pii: S1389-1723(21)00003-7. doi:, 10.1016/j.jbiosc.2021.01.003. PMID:33563496<ref>PMID:33563496</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6l0r" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aerpe]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ishikawa K]]	+	[[Category: Ishikawa, K]]
-	[[Category: Nakabayashi M]]	+	[[Category: Nakabayashi, M]]
-	[[Category: Nakamura T]]	+	[[Category: Nakamura, T]]
-	[[Category: Takeda E]]	+	[[Category: Takeda, E]]
		+	[[Category: Cysteine biosynthesis]]
		+	[[Category: Sulfhydrylase]]
		+	[[Category: Transferase]]

OCA at 11:08, 23 September 2020

OCA — Wed, 23 Sep 2020 11:08:30 GMT

←Older revision		Revision as of 11:08, 23 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6l0r is ~~ON HOLD until Paper Publication~~	+	==Crystal Structure of the O-Phosphoserine Sulfhydrylase from Aeropyrum pernix Complexed with O-Acetylserine==
-		+	<StructureSection load='6l0r' size='340' side='right'caption='[[6l0r]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L0R OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L0R FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l0r OCA], [http://pdbe.org/6l0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l0r RCSB], [http://www.ebi.ac.uk/pdbsum/6l0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l0r ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ishikawa K]]
		+	[[Category: Nakabayashi M]]
		+	[[Category: Nakamura T]]
		+	[[Category: Takeda E]]

OCA at 04:16, 16 October 2019

OCA — Wed, 16 Oct 2019 04:16:34 GMT

←Older revision		Revision as of 04:16, 16 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6l0r is ON HOLD	+	The entry 6l0r is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6l0r" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 07:06:36 GMT

Protected "6l0r" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:06, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6l0r is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Oct 2019 07:06:34 GMT

New page: '''Unreleased structure''' The entry 6l0r is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6l0r is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]