6l3n - Revision history

OCA at 10:50, 22 November 2023

OCA — Wed, 22 Nov 2023 10:50:58 GMT

←Older revision		Revision as of 10:50, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6l3n' size='340' side='right'caption='[[6l3n]], [[Resolution\|resolution]] 1.83Å' scene=''>		<StructureSection load='6l3n' size='340' side='right'caption='[[6l3n]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6l3n]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Actpa Actpa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6L3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l3n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinosynnema_pretiosum_subsp._auranticum Actinosynnema pretiosum subsp. auranticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L3N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DXX:METHYLMALONIC+ACID'>DXX</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.83Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AsmAT3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42198 ACTPA])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DXX:METHYLMALONIC+ACID'>DXX</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6l3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3n OCA], [~~http~~://pdbe.org/6l3n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6l3n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6l3n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3n OCA], [https://pdbe.org/6l3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l3n RCSB], [https://www.ebi.ac.uk/pdbsum/6l3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3n ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1U9Y7T8_9PSEU A0A1U9Y7T8_9PSEU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Actpa~~]]	+	[[Category: Actinosynnema pretiosum subsp. auranticum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Zhang, F]]	+	[[Category: Zhang F]]
-	[[Category: Zheng, J]]	+	[[Category: Zheng J]]
-	~~[[Category: Acp-linked extender unit]]~~	+
-	~~[[Category: Acyltransferase]]~~	+
-	~~[[Category: Biosynthesis]]~~	+
-	~~[[Category: Carrier specificity]]~~	+
-	~~[[Category: Polyketide]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:58, 29 April 2020

OCA — Wed, 29 Apr 2020 06:58:52 GMT

←Older revision		Revision as of 06:58, 29 April 2020
Line 3:		Line 3:
	<StructureSection load='6l3n' size='340' side='right'caption='[[6l3n]], [[Resolution\|resolution]] 1.83Å' scene=''>		<StructureSection load='6l3n' size='340' side='right'caption='[[6l3n]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6l3n]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3N OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6L3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l3n]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Actpa Actpa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3N OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L3N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DXX:METHYLMALONIC+ACID'>DXX</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DXX:METHYLMALONIC+ACID'>DXX</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6l3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3n OCA], [http://pdbe.org/6l3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3n RCSB], [http://www.ebi.ac.uk/pdbsum/6l3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3n ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AsmAT3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=42198 ACTPA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3n OCA], [http://pdbe.org/6l3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3n RCSB], [http://www.ebi.ac.uk/pdbsum/6l3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Actpa]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Zhang, F]]		[[Category: Zhang, F]]

OCA at 07:42, 18 December 2019

OCA — Wed, 18 Dec 2019 07:42:23 GMT

←Older revision		Revision as of 07:42, 18 December 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6l3n is ~~ON HOLD~~	+	==Crystal Structure of the acyltransferase domain from the third module of the ansamitocin polyketide synthase==
		+	<StructureSection load='6l3n' size='340' side='right'caption='[[6l3n]], [[Resolution\|resolution]] 1.83Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6l3n]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L3N FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DXX:METHYLMALONIC+ACID'>DXX</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3n OCA], [http://pdbe.org/6l3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3n RCSB], [http://www.ebi.ac.uk/pdbsum/6l3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3n ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A few acyltransferase (AT) domains of modular polyketide synthases (PKSs) recruit acyl carrier protein (ACP)-linked extender units with unusual C2 substituents to confer functionalities that are not available in coenzyme A (CoA)-linked ones. Here, an AT specific for methoxymalonyl (MOM)-ACP in the third module of the ansamitocin PKS was structurally and biochemically characterized. The AT uses a conserved tryptophan at the entrance of the substrate binding tunnel to discriminate between different carriers. A W275R mutation switches its carrier specificity from the ACP protein to the CoA molecule. The acyl-AT complex structures clearly show that the MOM-ACP accepted by the AT has the 2S instead of the opposite 2R stereochemistry that is predicted according to the biosynthetic derivation from a D-glycolytic intermediate. Together, these results reveal the structural basis of ATs recognizing ACP-linked extender units in polyketide biosynthesis.

-	~~Authors: Zhang~~, F., ~~Zheng~~, J.	+	Structural and Biochemical insights to the Recruitment of Acyl Carrier Protein-linked Extender Units in Ansamitocin Biosynthesis.,Zhang F, Ji H, Ali I, Deng Z, Bai L, Zheng J Chembiochem. 2019 Nov 27. doi: 10.1002/cbic.201900628. PMID:31777147<ref>PMID:31777147</ref>

-	~~Description: Crystal Structure~~ of the ~~acyltransferase domain from the third module~~ of ~~the ansamitocin polyketide synthase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Zheng, J~~]]	+	<div class="pdbe-citations 6l3n" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Zhang, F]]		[[Category: Zhang, F]]
		+	[[Category: Zheng, J]]
		+	[[Category: Acp-linked extender unit]]
		+	[[Category: Acyltransferase]]
		+	[[Category: Biosynthesis]]
		+	[[Category: Carrier specificity]]
		+	[[Category: Polyketide]]
		+	[[Category: Transferase]]

OCA: Protected "6l3n" [edit=sysop:move=sysop]

OCA — Wed, 23 Oct 2019 04:49:53 GMT

Protected "6l3n" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 23 October 2019

OCA: New page: '''Unreleased structure''' The entry 6l3n is ON HOLD Authors: Zhang, F., Zheng, J. Description: Crystal Structure of the acyltransferase domain from the third module of the ansamitocin...

OCA — Wed, 23 Oct 2019 04:49:52 GMT

New page: '''Unreleased structure''' The entry 6l3n is ON HOLD Authors: Zhang, F., Zheng, J. Description: Crystal Structure of the acyltransferase domain from the third module of the ansamitocin...

New page

'''Unreleased structure'''

The entry 6l3n is ON HOLD

Authors: Zhang, F., Zheng, J.

Description: Crystal Structure of the acyltransferase domain from the third module of the ansamitocin polyketide synthase
[[Category: Unreleased Structures]]
[[Category: Zheng, J]]
[[Category: Zhang, F]]