6l3t - Revision history

OCA at 07:21, 30 September 2020

OCA — Wed, 30 Sep 2020 07:21:02 GMT

←Older revision		Revision as of 07:21, 30 September 2020
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	==Human Cx31.3/GJC3 connexin hemichannel in the absence of calcium==		==Human Cx31.3/GJC3 connexin hemichannel in the absence of calcium==
-	<StructureSection load='6l3t' size='340' side='right'caption='[[6l3t]]' scene=''>	+	<StructureSection load='6l3t' size='340' side='right'caption='[[6l3t]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L3T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l3t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L3T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3t OCA], [http://pdbe.org/6l3t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3t RCSB], [http://www.ebi.ac.uk/pdbsum/6l3t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3t ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMN:LAURYL+MALTOSE+NEOPENTYL+GLYCOL'>LMN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GJC3, GJE1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3t OCA], [http://pdbe.org/6l3t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3t RCSB], [http://www.ebi.ac.uk/pdbsum/6l3t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3t ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CXG3_HUMAN CXG3_HUMAN]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Connexin family proteins assemble into hexameric channels called hemichannels/connexons, which function as transmembrane channels or dock together to form gap junction intercellular channels (GJIChs). We determined the cryo-electron microscopy structures of human connexin 31.3 (Cx31.3)/GJC3 hemichannels in the presence and absence of calcium ions and with a hearing-loss mutation R15G at 2.3-, 2.5-, and 2.6-A resolutions, respectively. Compared with available structures of GJICh in open conformation, Cx31.3 hemichannel shows substantial structural changes of highly conserved regions in the connexin family, including opening of calcium ion-binding tunnels, reorganization of salt-bridge networks, exposure of lipid-binding sites, and collocation of amino-terminal helices at the cytoplasmic entrance. We also found that the hemichannel has a pore with a diameter of ~8 A and selectively transports chloride ions. Our study provides structural insights into the permeant selectivity of Cx31.3 hemichannel.
		+
		+	Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel.,Lee HJ, Jeong H, Hyun J, Ryu B, Park K, Lim HH, Yoo J, Woo JS Sci Adv. 2020 Aug 28;6(35):eaba4996. doi: 10.1126/sciadv.aba4996. eCollection, 2020 Aug. PMID:32923625<ref>PMID:32923625</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6l3t" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hyun J]]	+	[[Category: Hyun, J]]
-	[[Category: Jeong H]]	+	[[Category: Jeong, H]]
-	[[Category: Lee HJ]]	+	[[Category: Lee, H J]]
-	[[Category: Ryu B]]	+	[[Category: Ryu, B]]
-	[[Category: Woo JS]]	+	[[Category: Woo, J S]]
		+	[[Category: Atp release]]
		+	[[Category: Gap junction]]
		+	[[Category: Hemichannel]]
		+	[[Category: Hexamer]]
		+	[[Category: Membrane protein]]

OCA at 09:29, 9 September 2020

OCA — Wed, 09 Sep 2020 09:29:42 GMT

←Older revision		Revision as of 09:29, 9 September 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6l3t ~~is ON HOLD~~	+	==Human Cx31.3/GJC3 connexin hemichannel in the absence of calcium==
-		+	<StructureSection load='6l3t' size='340' side='right'caption='[[6l3t]]' scene=''>
-	~~Authors~~: ~~Lee, H~~.J., ~~Jeong~~, H., ~~Ryu, B~~., ~~Hyun, J~~., ~~Woo, J~~.S.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L3T FirstGlance]. <br>
-	~~Description: Human Cx31.3~~/~~GJC3 connexin hemichannel in the absence of calcium~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3t OCA], [http://pdbe.org/6l3t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l3t RCSB], [http://www.ebi.ac.uk/pdbsum/6l3t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3t ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Lee, H.~~J]]	+	__TOC__
-	[[Category: ~~Woo, J.S~~]]	+	</StructureSection>
-	[[Category: ~~Hyun, J~~]]	+	[[Category: Large Structures]]
-	[[Category: Ryu, B]]	+	[[Category: Hyun J]]
-	[[Category: ~~Jeong, H~~]]	+	[[Category: Jeong H]]
		+	[[Category: Lee HJ]]
		+	[[Category: Ryu B]]
		+	[[Category: Woo JS]]

OCA: Protected "6l3t" [edit=sysop:move=sysop]

OCA — Wed, 23 Oct 2019 04:50:35 GMT

Protected "6l3t" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:50, 23 October 2019

OCA: New page: '''Unreleased structure''' The entry 6l3t is ON HOLD Authors: Lee, H.J., Jeong, H., Ryu, B., Hyun, J., Woo, J.S. Description: Human Cx31.3/GJC3 connexin hemichannel in the absence of c...

OCA — Wed, 23 Oct 2019 04:50:33 GMT

New page: '''Unreleased structure''' The entry 6l3t is ON HOLD Authors: Lee, H.J., Jeong, H., Ryu, B., Hyun, J., Woo, J.S. Description: Human Cx31.3/GJC3 connexin hemichannel in the absence of c...

New page

'''Unreleased structure'''

The entry 6l3t is ON HOLD

Authors: Lee, H.J., Jeong, H., Ryu, B., Hyun, J., Woo, J.S.

Description: Human Cx31.3/GJC3 connexin hemichannel in the absence of calcium
[[Category: Unreleased Structures]]
[[Category: Lee, H.J]]
[[Category: Woo, J.S]]
[[Category: Hyun, J]]
[[Category: Ryu, B]]
[[Category: Jeong, H]]