6l5s - Revision history

OCA at 10:51, 22 November 2023

OCA — Wed, 22 Nov 2023 10:51:52 GMT

←Older revision		Revision as of 10:51, 22 November 2023
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	<StructureSection load='6l5s' size='340' side='right'caption='[[6l5s]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='6l5s' size='340' side='right'caption='[[6l5s]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6l5s]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Glygl Glygl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L5S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6L5S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l5s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycyrrhiza_glabra Glycyrrhiza glabra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L5S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G50:3-(4-HYDROXYPHENYL)-1-(2,4,6-TRIHYDROXYPHENYL)PROPAN-1-ONE'>G50</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.914Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6l5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l5s OCA], [~~http~~://pdbe.org/6l5s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6l5s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6l5s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6l5s ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G50:3-(4-HYDROXYPHENYL)-1-(2,4,6-TRIHYDROXYPHENYL)PROPAN-1-ONE'>G50</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l5s OCA], [https://pdbe.org/6l5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l5s RCSB], [https://www.ebi.ac.uk/pdbsum/6l5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l5s ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glygl~~]]	+	[[Category: Glycyrrhiza glabra]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Li~~, F D~~]]	+	[[Category: Li FD]]
-	[[Category: Ye, M]]	+	[[Category: Ye M]]
-	[[Category: Zhang, M]]	+	[[Category: Zhang M]]
-	~~[[Category: Di-c-glycosyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:11, 4 March 2020

OCA — Wed, 04 Mar 2020 07:11:19 GMT

←Older revision		Revision as of 07:11, 4 March 2020
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	<StructureSection load='6l5s' size='340' side='right'caption='[[6l5s]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='6l5s' size='340' side='right'caption='[[6l5s]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6l5s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L5S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l5s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Glygl Glygl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L5S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G50:3-(4-HYDROXYPHENYL)-1-(2,4,6-TRIHYDROXYPHENYL)PROPAN-1-ONE'>G50</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G50:3-(4-HYDROXYPHENYL)-1-(2,4,6-TRIHYDROXYPHENYL)PROPAN-1-ONE'>G50</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l5s OCA], [http://pdbe.org/6l5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l5s RCSB], [http://www.ebi.ac.uk/pdbsum/6l5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l5s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l5s OCA], [http://pdbe.org/6l5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l5s RCSB], [http://www.ebi.ac.uk/pdbsum/6l5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l5s ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Glygl]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Li, F D]]		[[Category: Li, F D]]

OCA at 06:21, 19 February 2020

OCA — Wed, 19 Feb 2020 06:21:47 GMT

←Older revision		Revision as of 06:21, 19 February 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6l5s is ~~ON HOLD until Paper~~ Publication	+	==crystal structure of GgCGT in complex with UDP-Glu==
		+	<StructureSection load='6l5s' size='340' side='right'caption='[[6l5s]], [[Resolution\|resolution]] 1.91Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6l5s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L5S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G50:3-(4-HYDROXYPHENYL)-1-(2,4,6-TRIHYDROXYPHENYL)PROPAN-1-ONE'>G50</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l5s OCA], [http://pdbe.org/6l5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l5s RCSB], [http://www.ebi.ac.uk/pdbsum/6l5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l5s ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A highly efficient di-C-glycosyltransferase GgCGT was discovered from the medicinal plant Glycyrrhiza glabra. GgCGT catalyzes a two-step di-C-glycosylation of flopropione-containing substrates with conversion rates of >98%. To elucidate the catalytic mechanisms of GgCGT, we solved its crystal structures in complex with UDP-Glc, UDP-Gal, UDP/phloretin, and UDP/nothofagin, respectively. Structural analysis revealed that the sugar donor selectivity was controlled by the hydrogen-bond interactions of sugar hydroxyl groups with D390 and other key residues. The di-C-glycosylation capability of GgCGT was attributed to a spacious substrate-binding tunnel, and the G389K mutation could switch di- to mono-C-glycosylation. GgCGT is the first di-C-glycosyltransferase with a crystal structure, and the first C-glycosyltransferase with a complex structure containing a sugar acceptor. This work could benefit the development of efficient biocatalysts to synthesize C-glycosides with medicinal potential.

-	~~Authors~~:	+	Functional Characterization and Structural Basis of an Efficient Di-C-glycosyltransferase from Glycyrrhiza glabra.,Zhang M, Li FD, Li K, Wang ZL, Wang YX, He JB, Su HF, Zhang ZY, Chi CB, Shi XM, Yun CH, Zhang ZY, Liu ZM, Zhang LR, Yang DH, Ma M, Qiao X, Ye M J Am Chem Soc. 2020 Feb 11. doi: 10.1021/jacs.9b12211. PMID:31986016<ref>PMID:31986016</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6l5s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Li, F D]]
		+	[[Category: Ye, M]]
		+	[[Category: Zhang, M]]
		+	[[Category: Di-c-glycosyltransferase]]
		+	[[Category: Transferase]]

OCA at 09:14, 13 November 2019

OCA — Wed, 13 Nov 2019 09:14:19 GMT

←Older revision		Revision as of 09:14, 13 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6l5s is ON HOLD	+	The entry 6l5s is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6l5s" [edit=sysop:move=sysop]

OCA — Wed, 30 Oct 2019 07:22:18 GMT

Protected "6l5s" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:22, 30 October 2019

OCA: New page: '''Unreleased structure''' The entry 6l5s is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 30 Oct 2019 07:22:16 GMT

New page: '''Unreleased structure''' The entry 6l5s is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6l5s is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]