6l8t - Revision history

OCA at 10:53, 22 November 2023

OCA — Wed, 22 Nov 2023 10:53:17 GMT

←Older revision		Revision as of 10:53, 22 November 2023
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	<StructureSection load='6l8t' size='340' side='right'caption='[[6l8t]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='6l8t' size='340' side='right'caption='[[6l8t]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6l8t]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L8T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6L8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l8t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L8T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l8t OCA], [~~http~~://pdbe.org/6l8t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6l8t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6l8t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6l8t ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.766Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l8t OCA], [https://pdbe.org/6l8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l8t RCSB], [https://www.ebi.ac.uk/pdbsum/6l8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l8t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	*[[Sandbox 20009\|Sandbox 20009]]		*[[Sandbox 20009\|Sandbox 20009]]
	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]		*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gu, Y]]	+	[[Category: Gu Y]]
-	[[Category: He~~, M Z~~]]	+	[[Category: He MZ]]
-	[[Category: Li~~, S W]]~~	+	[[Category: Li SW]]
-	~~[[Category: Antibody]]~~	+
-	~~[[Category: Immune system~~]]	+

OCA at 09:48, 9 September 2020

OCA — Wed, 09 Sep 2020 09:48:44 GMT

←Older revision		Revision as of 09:48, 9 September 2020
Line 3:		Line 3:
	<StructureSection load='6l8t' size='340' side='right'caption='[[6l8t]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='6l8t' size='340' side='right'caption='[[6l8t]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6l8t]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L8T OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6L8T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6l8t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L8T OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6L8T FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l8t OCA], [http://pdbe.org/6l8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l8t RCSB], [http://www.ebi.ac.uk/pdbsum/6l8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l8t ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l8t OCA], [http://pdbe.org/6l8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l8t RCSB], [http://www.ebi.ac.uk/pdbsum/6l8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l8t ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have reported that E6F6, a mouse monoclonal antibody, is a promising treatment option for patients with chronic hepatitis B (CHB). A humanized E6F6 antibody B11 with affinity loss was obtained by CDR-grafting approach. To address this issue, in silico affinity maturation through scanning mutagenesis using CHARMM force field methods was performed on an predicted immune complex model of the B11:HBsAg. We chose four variants with top increased interaction energy for further characterization. The antibody huE6F6-1 within two point mutations (Heavy Chain: Asp65Val; His66Leu) was identified to restore the parental antibody's high binding affinity, neutralization activity, and potent efficacy of viral suppression in vivo. Crystal structure (1.8 A resolution) based molecular docking proved more stabilized and compact hydrogen bond interactions formed in huE6F6-1.The smaller and dispersed HBV immune complexes of huE6F6-1 by electron microscopy suggested it will have the same therapeutic efficacy as the parental E6F6 mAb. Preclinical study and pharmacokinetics of huE6F6-1 demonstrated that it is a stable and desirable lead candidate to improve the clinical management of CHB. Notably, our structure guided approach may facilitate the humanization and affinity maturation of other rodent antibody candidates during drug development.
		+
		+	Structure guided maturation of a novel humanized anti-HBV antibody and its preclinical development.,Zhou B, Xia L, Zhang T, You M, Huang Y, He M, Su R, Tang J, Zhang J, Li S, An Z, Yuan Q, Luo W, Xia N Antiviral Res. 2020 Aug;180:104757. doi: 10.1016/j.antiviral.2020.104757. Epub, 2020 Mar 12. PMID:32171857<ref>PMID:32171857</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6l8t" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Sandbox 20009\|Sandbox 20009]]
		+	*[[3D structures of non-human antibody\|3D structures of non-human antibody]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Gu, Y]]		[[Category: Gu, Y]]

OCA at 09:14, 26 February 2020

OCA — Wed, 26 Feb 2020 09:14:29 GMT

←Older revision		Revision as of 09:14, 26 February 2020
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-	'''Unreleased structure'''

-	~~The entry 6l8t is ON HOLD~~	+	==Crystal structure of the Fab fragment of a humanized HBV therapeutic antibody==
-		+	<StructureSection load='6l8t' size='340' side='right'caption='[[6l8t]], [[Resolution\|resolution]] 1.77Å' scene=''>
-	~~Authors: He, M.Z., Gu, Y., Li, S.W.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6l8t]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L8T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L8T FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the Fab fragment of a humanized HBV therapeutic antibody	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l8t OCA], [http://pdbe.org/6l8t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l8t RCSB], [http://www.ebi.ac.uk/pdbsum/6l8t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l8t ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: Li, S.W]]	+	__TOC__
-	[[Category: ~~He, M.Z~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Gu, Y]]		[[Category: Gu, Y]]
		+	[[Category: He, M Z]]
		+	[[Category: Li, S W]]
		+	[[Category: Antibody]]
		+	[[Category: Immune system]]

OCA: Protected "6l8t" [edit=sysop:move=sysop]

OCA — Wed, 13 Nov 2019 09:18:20 GMT

Protected "6l8t" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:18, 13 November 2019

OCA: New page: '''Unreleased structure''' The entry 6l8t is ON HOLD Authors: He, M.Z., Gu, Y., Li, S.W. Description: Crystal structure of the Fab fragment of a humanized HBV therapeutic antibody [[Ca...

OCA — Wed, 13 Nov 2019 09:18:19 GMT

New page: '''Unreleased structure''' The entry 6l8t is ON HOLD Authors: He, M.Z., Gu, Y., Li, S.W. Description: Crystal structure of the Fab fragment of a humanized HBV therapeutic antibody [[Ca...

New page

'''Unreleased structure'''

The entry 6l8t is ON HOLD

Authors: He, M.Z., Gu, Y., Li, S.W.

Description: Crystal structure of the Fab fragment of a humanized HBV therapeutic antibody
[[Category: Unreleased Structures]]
[[Category: Li, S.W]]
[[Category: He, M.Z]]
[[Category: Gu, Y]]