OCA at 06:48, 27 November 2019

OCA — Wed, 27 Nov 2019 06:48:59 GMT

←Older revision		Revision as of 06:48, 27 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6lav is ~~ON HOLD~~	+	==MicroED structure of lysozyme at 1.73A determained using crystal lamellas prepared by focused ion beam milling==
		+	<StructureSection load='6lav' size='340' side='right'caption='[[6lav]], [[Resolution\|resolution]] 1.73Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6lav]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LAV FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6lav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lav OCA], [http://pdbe.org/6lav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lav RCSB], [http://www.ebi.ac.uk/pdbsum/6lav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lav ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Electron diffraction provides a powerful tool to solve the structures of small protein crystals. However, strong interactions between the electrons and the materials limit the application of the electron crystallographic method on large protein crystals with micrometer or larger sizes. Here, we used the focused ion beam (FIB) equipped on the scanning electron microscope (SEM) to mill a large crystal to thin lamella. The influences of the milling on the crystal lamella were observed and investigated, including radiation damage on the crystal surface during the FIB imaging, deformation of the thin crystal lamella, and variation in the diffraction intensities under electron radiation. These observations provide important information to optimize the FIB milling, and hence is important to obtain high-quality crystal samples for routine structure determination of protein crystals using the electron cryo-microscope.

-	~~Authors~~:	+	Using focus ion beam to prepare crystal lamella for electron diffraction.,Zhou H, Luo Z, Li X J Struct Biol. 2019 Mar 1;205(3):59-64. doi: 10.1016/j.jsb.2019.02.004. Epub 2019, Feb 20. PMID:30794865<ref>PMID:30794865</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6lav" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
		+	[[Category: Lysozyme]]
		+	[[Category: Li, X]]
		+	[[Category: Luo, Z]]
		+	[[Category: Zhou, H]]
		+	[[Category: Crystal lamella]]
		+	[[Category: Focused ion beam]]
		+	[[Category: Hydrolase]]
		+	[[Category: Microed]]

OCA: Protected "6lav" [edit=sysop:move=sysop]

OCA — Wed, 20 Nov 2019 13:29:49 GMT

Protected "6lav" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:29, 20 November 2019

OCA: New page: '''Unreleased structure''' The entry 6lav is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Nov 2019 13:29:47 GMT

New page: '''Unreleased structure''' The entry 6lav is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6lav is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6lav - Revision history

OCA at 06:48, 27 November 2019

OCA: Protected "6lav" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6lav is ON HOLD Authors: Description: Category: Unreleased Structures