6lg1 - Revision history

OCA at 10:59, 22 November 2023

OCA — Wed, 22 Nov 2023 10:59:35 GMT

←Older revision		Revision as of 10:59, 22 November 2023
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	<StructureSection load='6lg1' size='340' side='right'caption='[[6lg1]], [[Resolution\|resolution]] 3.05Å' scene=''>		<StructureSection load='6lg1' size='340' side='right'caption='[[6lg1]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lg1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dotted_duckmeat Dotted duckmeat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LG1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LG1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lg1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Landoltia_punctata Landoltia punctata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LG1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.047Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6lg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lg1 OCA], [~~http~~://pdbe.org/6lg1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lg1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lg1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lg1 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lg1 OCA], [https://pdbe.org/6lg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lg1 RCSB], [https://www.ebi.ac.uk/pdbsum/6lg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lg1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dotted duckmeat~~]]	+	[[Category: Landoltia punctata]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gao~~, H M~~]]	+	[[Category: Gao HM]]
-	[[Category: Yun~~, C H]]~~	+	[[Category: Yun CH]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Lpcgta]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Udp~~]]	+

OCA at 08:03, 2 December 2020

OCA — Wed, 02 Dec 2020 08:03:47 GMT

←Older revision		Revision as of 08:03, 2 December 2020
Line 1:		Line 1:

	==Crystal structure of LpCGTa in complex with UDP==		==Crystal structure of LpCGTa in complex with UDP==
-	<StructureSection load='6lg1' size='340' side='right'caption='[[6lg1]]' scene=''>	+	<StructureSection load='6lg1' size='340' side='right'caption='[[6lg1]], [[Resolution\|resolution]] 3.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LG1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LG1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lg1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dotted_duckmeat Dotted duckmeat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LG1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LG1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lg1 OCA], [http://pdbe.org/6lg1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lg1 RCSB], [http://www.ebi.ac.uk/pdbsum/6lg1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lg1 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lg1 OCA], [http://pdbe.org/6lg1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lg1 RCSB], [http://www.ebi.ac.uk/pdbsum/6lg1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lg1 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Schaftoside and isoschaftoside are bioactive natural products widely distributed in higher plants including cereal crops and medicinal herbs. Their biosynthesis may be related with plant defense. However, little is known on the glycosylation biosynthetic pathway of these flavonoid di-C-glycosides with different sugar residues. Herein, we report that the biosynthesis of (iso)schaftosides is sequentially catalyzed by two C-glycosyltransferases (CGTs), i.e., CGTa for C-glucosylation of the 2-hydroxyflavanone aglycone and CGTb for C-arabinosylation of the mono-C-glucoside. The two enzymes of the same plant exhibit high homology but remarkably different sugar acceptor and donor selectivities. A total of 14 CGTa and CGTb enzymes were cloned and characterized from seven dicot and monocot plants, including Scutellaria baicalensis, Glycyrrhiza uralensis, Oryza sativa ssp. japonica, and Zea mays, and the in vivo functions for three enzymes were verified by RNA interference and overexpression. Through transcriptome analysis, we found homologous genes in 119 other plants, indicating this pathway is general for the biosynthesis of (iso)schaftosides. Furthermore, we resolved the crystal structures of five CGTs and realized the functional switch of SbCGTb to SbCGTa by structural analysis and mutagenesis of key amino acids. The CGT enzymes discovered in this paper allow efficient synthesis of (iso)schaftosides, and the general glycosylation pathway presents a platform to study the chemical defense mechanisms of higher plants.
		+
		+	Dissection of the general two-step di-C-glycosylation pathway for the biosynthesis of (iso)schaftosides in higher plants.,Wang ZL, Gao HM, Wang S, Zhang M, Chen K, Zhang YQ, Wang HD, Han BY, Xu LL, Song TQ, Yun CH, Qiao X, Ye M Proc Natl Acad Sci U S A. 2020 Nov 16. pii: 2012745117. doi:, 10.1073/pnas.2012745117. PMID:33199630<ref>PMID:33199630</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6lg1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Dotted duckmeat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gao HM]]	+	[[Category: Gao, H M]]
-	[[Category: Yun CH]]	+	[[Category: Yun, C H]]
		+	[[Category: Complex]]
		+	[[Category: Lpcgta]]
		+	[[Category: Transferase]]
		+	[[Category: Udp]]

OCA at 09:31, 18 November 2020

OCA — Wed, 18 Nov 2020 09:31:52 GMT

←Older revision		Revision as of 09:31, 18 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6lg1 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of LpCGTa in complex with UDP==
-		+	<StructureSection load='6lg1' size='340' side='right'caption='[[6lg1]]' scene=''>
-	~~Authors~~: ~~Gao~~, H.M., ~~Yun~~, C.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LG1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LG1 FirstGlance]. <br>
-	~~Description: Crystal structure of LpCGTa in complex with UDP~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lg1 OCA], [http://pdbe.org/6lg1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lg1 RCSB], [http://www.ebi.ac.uk/pdbsum/6lg1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lg1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Yun, C.H~~]]	+	__TOC__
-	[[Category: ~~Gao, H.M~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gao HM]]
		+	[[Category: Yun CH]]

OCA at 13:28, 22 January 2020

OCA — Wed, 22 Jan 2020 13:28:47 GMT

←Older revision		Revision as of 13:28, 22 January 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6lg1 is ON HOLD	+	The entry 6lg1 is ON HOLD until Paper Publication

	Authors: Gao, H.M., Yun, C.H.		Authors: Gao, H.M., Yun, C.H.

OCA: Protected "6lg1" [edit=sysop:move=sysop]

OCA — Wed, 11 Dec 2019 13:27:44 GMT

Protected "6lg1" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:27, 11 December 2019

OCA: New page: '''Unreleased structure''' The entry 6lg1 is ON HOLD Authors: Gao, H.M., Yun, C.H. Description: Crystal structure of LpCGTa in complex with UDP Category: Unreleased Structures [[Ca...

OCA — Wed, 11 Dec 2019 13:27:42 GMT

New page: '''Unreleased structure''' The entry 6lg1 is ON HOLD Authors: Gao, H.M., Yun, C.H. Description: Crystal structure of LpCGTa in complex with UDP Category: Unreleased Structures [[Ca...

New page

'''Unreleased structure'''

The entry 6lg1 is ON HOLD

Authors: Gao, H.M., Yun, C.H.

Description: Crystal structure of LpCGTa in complex with UDP
[[Category: Unreleased Structures]]
[[Category: Yun, C.H]]
[[Category: Gao, H.M]]