6lgw - Revision history

OCA at 14:41, 13 March 2024

OCA — Wed, 13 Mar 2024 14:41:31 GMT

←Older revision		Revision as of 14:41, 13 March 2024
Line 9:		Line 9:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/GLYCO_RABVC GLYCO_RABVC] Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells (By similarity).		[https://www.uniprot.org/uniprot/GLYCO_RABVC GLYCO_RABVC] Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells (By similarity).
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Rabies virus (RABV), the etiological agent for the lethal disease of rabies, is a deadly zoonotic pathogen. The RABV glycoprotein (RABV-G) is a key factor mediating virus entry and the major target of neutralizing antibodies. Here, we report the crystal structures of RABV-G solved in the free form at approximately pH-8.0 and in the complex form with a neutralizing antibody 523-11 at approximately pH-6.5, respectively. RABV-G has three domains, and the basic-to-acidic pH change results in large domain re-orientations and concomitant domain-linker re-constructions, switching it from a bent hairpin conformation into an extended conformation. During such low-pH-induced structural transitions, residues located in the domain-linker are found to play important roles in glycoprotein-mediated membrane fusion. Finally, the antibody interacts with RABV-G mainly through its heavy chain and binds to a bipartite conformational epitope in the viral protein for neutralization. These structures provide valuable information for vaccine and drug design.
-
-	Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody.,Yang F, Lin S, Ye F, Yang J, Qi J, Chen Z, Lin X, Wang J, Yue D, Cheng Y, Chen Z, Chen H, You Y, Zhang Z, Yang Y, Yang M, Sun H, Li Y, Cao Y, Yang S, Wei Y, Gao GF, Lu G Cell Host Microbe. 2020 Jan 23. pii: S1931-3128(19)30641-9. doi:, 10.1016/j.chom.2019.12.012. PMID:32004500<ref>PMID:32004500</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 6lgw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Antibody 3D structures\|Antibody 3D structures]]		*[[Antibody 3D structures\|Antibody 3D structures]]
	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]		*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:00, 22 November 2023

OCA — Wed, 22 Nov 2023 11:00:04 GMT

←Older revision		Revision as of 11:00, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6lgw' size='340' side='right'caption='[[6lgw]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6lgw' size='340' side='right'caption='[[6lgw]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lgw]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~] and [~~http~~://en.wikipedia.org/wiki/Rabies_lyssavirus Rabies lyssavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LGW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6LGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lgw]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rabies_lyssavirus Rabies lyssavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LGW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6lgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lgw OCA], [~~http~~://pdbe.org/6lgw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lgw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lgw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lgw ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9037Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lgw OCA], [https://pdbe.org/6lgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lgw RCSB], [https://www.ebi.ac.uk/pdbsum/6lgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lgw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GLYCO_RABVC GLYCO_RABVC] Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6lgw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6lgw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 20:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
	[[Category: Rabies lyssavirus]]		[[Category: Rabies lyssavirus]]
-	[[Category: Cao, Y]]	+	[[Category: Cao Y]]
-	[[Category: Chen, H]]	+	[[Category: Chen H]]
-	[[Category: Chen~~, Z J~~]]	+	[[Category: Chen ZJ]]
-	[[Category: Chen~~, Z M~~]]	+	[[Category: Chen ZM]]
-	[[Category: Cheng~~, Y W~~]]	+	[[Category: Cheng YW]]
-	[[Category: Gao~~, G F~~]]	+	[[Category: Gao GF]]
-	[[Category: Li~~, Y H~~]]	+	[[Category: Li YH]]
-	[[Category: Lin, S]]	+	[[Category: Lin S]]
-	[[Category: Lin, X]]	+	[[Category: Lin X]]
-	[[Category: Lu~~, G W~~]]	+	[[Category: Lu GW]]
-	[[Category: Qi~~, J X~~]]	+	[[Category: Qi JX]]
-	[[Category: Sun~~, H L~~]]	+	[[Category: Sun HL]]
-	[[Category: Wang~~, J C~~]]	+	[[Category: Wang JC]]
-	[[Category: Wei~~, Y Q~~]]	+	[[Category: Wei YQ]]
-	[[Category: Yang~~, F L~~]]	+	[[Category: Yang FL]]
-	[[Category: Yang, J]]	+	[[Category: Yang J]]
-	[[Category: Yang, M]]	+	[[Category: Yang M]]
-	[[Category: Yang~~, S Y~~]]	+	[[Category: Yang SY]]
-	[[Category: Yang, Y]]	+	[[Category: Yang Y]]
-	[[Category: Ye, F]]	+	[[Category: Ye F]]
-	[[Category: You, Y]]	+	[[Category: You Y]]
-	[[Category: Yue, D]]	+	[[Category: Yue D]]
-	[[Category: Zhang~~, Z L]]~~	+	[[Category: Zhang ZL]]
-	~~[[Category: Functional class]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 09:34, 1 April 2020

OCA — Wed, 01 Apr 2020 09:34:05 GMT

←Older revision		Revision as of 09:34, 1 April 2020
Line 3:		Line 3:
	<StructureSection load='6lgw' size='340' side='right'caption='[[6lgw]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6lgw' size='340' side='right'caption='[[6lgw]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lgw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LGW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lgw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Rabies_lyssavirus Rabies lyssavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LGW FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6lgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lgw OCA], [http://pdbe.org/6lgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lgw RCSB], [http://www.ebi.ac.uk/pdbsum/6lgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lgw ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6lgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lgw OCA], [http://pdbe.org/6lgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lgw RCSB], [http://www.ebi.ac.uk/pdbsum/6lgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lgw ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
		+	[[Category: Rabies lyssavirus]]
	[[Category: Cao, Y]]		[[Category: Cao, Y]]
	[[Category: Chen, H]]		[[Category: Chen, H]]

OCA at 06:22, 19 February 2020

OCA — Wed, 19 Feb 2020 06:22:39 GMT

←Older revision		Revision as of 06:22, 19 February 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6lgw is ~~ON HOLD until Paper~~ Publication	+	==Structure of Rabies virus glycoprotein in complex with neutralizing antibody 523-11 at acidic pH==
		+	<StructureSection load='6lgw' size='340' side='right'caption='[[6lgw]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6lgw]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LGW FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6lgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lgw OCA], [http://pdbe.org/6lgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lgw RCSB], [http://www.ebi.ac.uk/pdbsum/6lgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lgw ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rabies virus (RABV), the etiological agent for the lethal disease of rabies, is a deadly zoonotic pathogen. The RABV glycoprotein (RABV-G) is a key factor mediating virus entry and the major target of neutralizing antibodies. Here, we report the crystal structures of RABV-G solved in the free form at approximately pH-8.0 and in the complex form with a neutralizing antibody 523-11 at approximately pH-6.5, respectively. RABV-G has three domains, and the basic-to-acidic pH change results in large domain re-orientations and concomitant domain-linker re-constructions, switching it from a bent hairpin conformation into an extended conformation. During such low-pH-induced structural transitions, residues located in the domain-linker are found to play important roles in glycoprotein-mediated membrane fusion. Finally, the antibody interacts with RABV-G mainly through its heavy chain and binds to a bipartite conformational epitope in the viral protein for neutralization. These structures provide valuable information for vaccine and drug design.

-	~~Authors~~:	+	Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody.,Yang F, Lin S, Ye F, Yang J, Qi J, Chen Z, Lin X, Wang J, Yue D, Cheng Y, Chen Z, Chen H, You Y, Zhang Z, Yang Y, Yang M, Sun H, Li Y, Cao Y, Yang S, Wei Y, Gao GF, Lu G Cell Host Microbe. 2020 Jan 23. pii: S1931-3128(19)30641-9. doi:, 10.1016/j.chom.2019.12.012. PMID:32004500<ref>PMID:32004500</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6lgw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cao, Y]]
		+	[[Category: Chen, H]]
		+	[[Category: Chen, Z J]]
		+	[[Category: Chen, Z M]]
		+	[[Category: Cheng, Y W]]
		+	[[Category: Gao, G F]]
		+	[[Category: Li, Y H]]
		+	[[Category: Lin, S]]
		+	[[Category: Lin, X]]
		+	[[Category: Lu, G W]]
		+	[[Category: Qi, J X]]
		+	[[Category: Sun, H L]]
		+	[[Category: Wang, J C]]
		+	[[Category: Wei, Y Q]]
		+	[[Category: Yang, F L]]
		+	[[Category: Yang, J]]
		+	[[Category: Yang, M]]
		+	[[Category: Yang, S Y]]
		+	[[Category: Yang, Y]]
		+	[[Category: Ye, F]]
		+	[[Category: You, Y]]
		+	[[Category: Yue, D]]
		+	[[Category: Zhang, Z L]]
		+	[[Category: Functional class]]
		+	[[Category: Viral protein]]

OCA at 06:32, 1 January 2020

OCA — Wed, 01 Jan 2020 06:32:02 GMT

←Older revision		Revision as of 06:32, 1 January 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6lgw is ON HOLD	+	The entry 6lgw is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6lgw" [edit=sysop:move=sysop]

OCA — Wed, 11 Dec 2019 13:29:56 GMT

Protected "6lgw" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:29, 11 December 2019

OCA: New page: '''Unreleased structure''' The entry 6lgw is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 11 Dec 2019 13:29:54 GMT

New page: '''Unreleased structure''' The entry 6lgw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6lgw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]