6lhw - Revision history

OCA at 10:46, 27 March 2024

OCA — Wed, 27 Mar 2024 10:46:25 GMT

←Older revision		Revision as of 10:46, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6lhw' size='340' side='right'caption='[[6lhw]], [[Resolution\|resolution]] 4.84Å' scene=''>		<StructureSection load='6lhw' size='340' side='right'caption='[[6lhw]], [[Resolution\|resolution]] 4.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lhw]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~African_clawed_frog African clawed frog~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LHW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lhw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LHW FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.84Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">fanca, FANCA ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=8355 African clawed frog])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lhw OCA], [https://pdbe.org/6lhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lhw RCSB], [https://www.ebi.ac.uk/pdbsum/6lhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lhw ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6lhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lhw OCA], [~~http~~://pdbe.org/6lhw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lhw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lhw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lhw ProSAT]</span></td></tr>	+
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Monoubiquitination of the Fanconi anemia complementation group D2 (FANCD2) protein by the FA core ubiquitin ligase complex is the central event in the FA pathway. FANCA and FANCG play major roles in the nuclear localization of the FA core complex. Mutations of these two genes are the most frequently observed genetic alterations in FA patients, and most point mutations in FANCA are clustered in the C-terminal domain (CTD). To understand the basis of the FA-associated FANCA mutations, we determined the cryo-electron microscopy (EM) structures of Xenopus laevis FANCA alone at 3.35 A and 3.46 A resolution and two distinct FANCA-FANCG complexes at 4.59 and 4.84 A resolution, respectively. The FANCA CTD adopts an arc-shaped solenoid structure that forms a pseudo-symmetric dimer through its outer surface. FA- and cancer-associated point mutations are widely distributed over the CTD. The two different complex structures capture independent interactions of FANCG with either FANCA C-terminal HEAT repeats, or the N-terminal region. We show that mutations that disturb either of these two interactions prevent the nuclear localization of FANCA, thereby leading to an FA pathway defect. The structure provides insights into the function of FANCA CTD, and provides a framework for understanding FA- and cancer-associated mutations.
-
-	Structural basis of the fanconi anemia-associated mutations within the FANCA and FANCG complex.,Jeong E, Lee SG, Kim HS, Yang J, Shin J, Kim Y, Kim J, Scharer OD, Kim Y, Yeo JE, Kim HM, Cho Y Nucleic Acids Res. 2020 Jan 31. pii: 5718259. doi: 10.1093/nar/gkaa062. PMID:32002546<ref>PMID:32002546</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 6lhw" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: African clawed frog]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cho, Y]]	+	[[Category: Xenopus laevis]]
-	[[Category: Jeong, E]]	+	[[Category: Cho Y]]
-	[[Category: Kim, H]]	+	[[Category: Jeong E]]
-	[[Category: Kim, J]]	+	[[Category: Kim H]]
-	[[Category: Kim, Y]]	+	[[Category: Kim J]]
-	[[Category: Lee, S]]	+	[[Category: Kim Y]]
-	[[Category: Scharer, O]]	+	[[Category: Lee S]]
-	[[Category: Shin, J]]	+	[[Category: Scharer O]]
-	~~[[Category: Dna repair]]~~	+	[[Category: Shin J]]
-	~~[[Category: Fanconi anemia complementation group some]]~~	+
-	~~[[Category: Fanconi anemia core protein]]~~	+
-	~~[[Category: Interstrand crosslink repair]]~~	+
-	~~[[Category: Nuclear localization~~]]	+

OCA at 07:17, 8 April 2020

OCA — Wed, 08 Apr 2020 07:17:54 GMT

←Older revision		Revision as of 07:17, 8 April 2020
Line 3:		Line 3:
	<StructureSection load='6lhw' size='340' side='right'caption='[[6lhw]], [[Resolution\|resolution]] 4.84Å' scene=''>		<StructureSection load='6lhw' size='340' side='right'caption='[[6lhw]], [[Resolution\|resolution]] 4.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lhw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LHW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6LHW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lhw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LHW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LHW FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6lhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lhw OCA], [http://pdbe.org/6lhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lhw RCSB], [http://www.ebi.ac.uk/pdbsum/6lhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lhw ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fanca, FANCA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 African clawed frog])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lhw OCA], [http://pdbe.org/6lhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lhw RCSB], [http://www.ebi.ac.uk/pdbsum/6lhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lhw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: African clawed frog]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cho, Y]]		[[Category: Cho, Y]]

OCA at 10:08, 27 March 2020

OCA — Fri, 27 Mar 2020 10:08:37 GMT

←Older revision		Revision as of 10:08, 27 March 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6lhw is ~~ON HOLD~~	+	==Structure of N-terminal and C-terminal domains of FANCA==
		+	<StructureSection load='6lhw' size='340' side='right'caption='[[6lhw]], [[Resolution\|resolution]] 4.84Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6lhw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LHW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LHW FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6lhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lhw OCA], [http://pdbe.org/6lhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lhw RCSB], [http://www.ebi.ac.uk/pdbsum/6lhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lhw ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Monoubiquitination of the Fanconi anemia complementation group D2 (FANCD2) protein by the FA core ubiquitin ligase complex is the central event in the FA pathway. FANCA and FANCG play major roles in the nuclear localization of the FA core complex. Mutations of these two genes are the most frequently observed genetic alterations in FA patients, and most point mutations in FANCA are clustered in the C-terminal domain (CTD). To understand the basis of the FA-associated FANCA mutations, we determined the cryo-electron microscopy (EM) structures of Xenopus laevis FANCA alone at 3.35 A and 3.46 A resolution and two distinct FANCA-FANCG complexes at 4.59 and 4.84 A resolution, respectively. The FANCA CTD adopts an arc-shaped solenoid structure that forms a pseudo-symmetric dimer through its outer surface. FA- and cancer-associated point mutations are widely distributed over the CTD. The two different complex structures capture independent interactions of FANCG with either FANCA C-terminal HEAT repeats, or the N-terminal region. We show that mutations that disturb either of these two interactions prevent the nuclear localization of FANCA, thereby leading to an FA pathway defect. The structure provides insights into the function of FANCA CTD, and provides a framework for understanding FA- and cancer-associated mutations.

-	~~Authors~~:	+	Structural basis of the fanconi anemia-associated mutations within the FANCA and FANCG complex.,Jeong E, Lee SG, Kim HS, Yang J, Shin J, Kim Y, Kim J, Scharer OD, Kim Y, Yeo JE, Kim HM, Cho Y Nucleic Acids Res. 2020 Jan 31. pii: 5718259. doi: 10.1093/nar/gkaa062. PMID:32002546<ref>PMID:32002546</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6lhw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cho, Y]]
		+	[[Category: Jeong, E]]
		+	[[Category: Kim, H]]
		+	[[Category: Kim, J]]
		+	[[Category: Kim, Y]]
		+	[[Category: Lee, S]]
		+	[[Category: Scharer, O]]
		+	[[Category: Shin, J]]
		+	[[Category: Dna repair]]
		+	[[Category: Fanconi anemia complementation group some]]
		+	[[Category: Fanconi anemia core protein]]
		+	[[Category: Interstrand crosslink repair]]
		+	[[Category: Nuclear localization]]

OCA at 02:29, 13 February 2020

OCA — Thu, 13 Feb 2020 02:29:07 GMT

←Older revision		Revision as of 02:29, 13 February 2020
Line 3:		Line 3:
	The entry 6lhw is ON HOLD		The entry 6lhw is ON HOLD

-	Authors: ~~Jeong, E.~~	+	Authors:

-	Description: ~~FANCA CTD and FANCG~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Jeong, E]]

OCA: Protected "6lhw" [edit=sysop:move=sysop]

OCA — Wed, 18 Dec 2019 05:42:09 GMT

Protected "6lhw" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:42, 18 December 2019

OCA: New page: '''Unreleased structure''' The entry 6lhw is ON HOLD Authors: Jeong, E. Description: FANCA CTD and FANCG Category: Unreleased Structures Category: Jeong, E

OCA — Wed, 18 Dec 2019 05:42:07 GMT

New page: '''Unreleased structure''' The entry 6lhw is ON HOLD Authors: Jeong, E. Description: FANCA CTD and FANCG Category: Unreleased Structures Category: Jeong, E

New page

'''Unreleased structure'''

The entry 6lhw is ON HOLD

Authors: Jeong, E.

Description: FANCA CTD and FANCG
[[Category: Unreleased Structures]]
[[Category: Jeong, E]]