6ljd - Revision history

OCA at 11:01, 22 November 2023

OCA — Wed, 22 Nov 2023 11:01:36 GMT

←Older revision		Revision as of 11:01, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='6ljd' size='340' side='right'caption='[[6ljd]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='6ljd' size='340' side='right'caption='[[6ljd]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ljd]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Phypo Phypo~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LJD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ljd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LJD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ljd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljd OCA], [~~http~~://pdbe.org/6ljd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ljd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ljd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljd ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ljd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljd OCA], [https://pdbe.org/6ljd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ljd RCSB], [https://www.ebi.ac.uk/pdbsum/6ljd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljd ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q94707_PHYPO Q94707_PHYPO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Phypo~~]]	+	[[Category: Physarum polycephalum]]
-	[[Category: Takeda, S]]	+	[[Category: Takeda S]]
-	~~[[Category: Actin filament severing]]~~	+
-	~~[[Category: Calcium regulation]]~~	+
-	~~[[Category: Cytosolic protein]]~~	+
-	~~[[Category: Fragmin]]~~	+
-	~~[[Category: Gelsolin family protein~~]]	+

OCA at 07:12, 15 April 2020

OCA — Wed, 15 Apr 2020 07:12:31 GMT

←Older revision		Revision as of 07:12, 15 April 2020
Line 3:		Line 3:
	<StructureSection load='6ljd' size='340' side='right'caption='[[6ljd]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='6ljd' size='340' side='right'caption='[[6ljd]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ljd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJD OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6LJD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ljd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Phypo Phypo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LJD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ljd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljd OCA], [http://pdbe.org/6ljd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ljd RCSB], [http://www.ebi.ac.uk/pdbsum/6ljd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ljd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljd OCA], [http://pdbe.org/6ljd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ljd RCSB], [http://www.ebi.ac.uk/pdbsum/6ljd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljd ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Phypo]]
	[[Category: Takeda, S]]		[[Category: Takeda, S]]
	[[Category: Actin filament severing]]		[[Category: Actin filament severing]]

OCA at 08:19, 1 January 2020

OCA — Wed, 01 Jan 2020 08:19:31 GMT

←Older revision		Revision as of 08:19, 1 January 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ljd is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of fragmin F2-F3 domains (calcium condition)==
		+	<StructureSection load='6ljd' size='340' side='right'caption='[[6ljd]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ljd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6LJD FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ljd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljd OCA], [http://pdbe.org/6ljd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ljd RCSB], [http://www.ebi.ac.uk/pdbsum/6ljd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljd ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gelsolin superfamily proteins, consisting of multiple domains (usually six), sever actin filaments and cap the barbed ends in a Ca(2+)-dependent manner. Two types of evolutionally conserved Ca(2+)-binding sites have been identified in this family; type-1 (between gelsolin and actin) and type-2 (within the gelsolin domain). Fragmin, a member in the slime mold Physarum polycephalum, consists of three domains (F1-F3) that are highly similar to the N-terminal half of mammalian gelsolin (G1-G3). Despite their similarities, the two proteins exhibit a significant difference in the Ca(2+) dependency; F1-F3 absolutely requires Ca(2+) for the filament severing whereas G1-G3 does not. In this study, we examined the strong dependency of fragmin on Ca(2+) using biochemical and structural approaches. Our co-sedimentation assay demonstrated that Ca(2+) significantly enhanced the binding of F2-F3 to actin. We determined the crystal structure of F2-F3 in the presence of Ca(2+). F2-F3 binds a total of three calcium ions; while two are located in type-2 sites within F2 or F3, the remaining one resides between the F2 long helix and the F3 short helix. The inter-domain Ca(2+)-coordination appears to stabilize F2-F3 in a closely packed configuration. Notably, the F3 long helix exhibits a bent conformation which is different from the straight G3 long helix in the presence of Ca(2+). Our results provide the first structural evidence for the existence of an unconventional Ca(2+)-binding site in the gelsolin superfamily proteins.

-	~~Authors~~:	+	Novel inter-domain Ca(2+)-binding site in the gelsolin superfamily protein fragmin.,Takeda S, Fujiwara I, Sugimoto Y, Oda T, Narita A, Maeda Y J Muscle Res Cell Motil. 2019 Dec 20. pii: 10.1007/s10974-019-09571-5. doi:, 10.1007/s10974-019-09571-5. PMID:31863323<ref>PMID:31863323</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6ljd" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Takeda, S]]
		+	[[Category: Actin filament severing]]
		+	[[Category: Calcium regulation]]
		+	[[Category: Cytosolic protein]]
		+	[[Category: Fragmin]]
		+	[[Category: Gelsolin family protein]]

OCA: Protected "6ljd" [edit=sysop:move=sysop]

OCA — Wed, 25 Dec 2019 07:05:33 GMT

Protected "6ljd" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:05, 25 December 2019

OCA: New page: '''Unreleased structure''' The entry 6ljd is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Dec 2019 07:05:31 GMT

New page: '''Unreleased structure''' The entry 6ljd is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ljd is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]