6ljq - Revision history

OCA at 11:02, 22 November 2023

OCA — Wed, 22 Nov 2023 11:02:21 GMT

←Older revision		Revision as of 11:02, 22 November 2023
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	<StructureSection load='6ljq' size='340' side='right'caption='[[6ljq]], [[Resolution\|resolution]] 1.49Å' scene=''>		<StructureSection load='6ljq' size='340' side='right'caption='[[6ljq]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ljq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ljq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LJQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BGC:BETA-D~~-~~GLUCOSE'>BGC</scene>~~, ~~<scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.49Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~LGALS16 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id~~=~~9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ljq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljq OCA], [~~http~~://pdbe.org/6ljq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ljq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ljq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ljq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljq OCA], [https://pdbe.org/6ljq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ljq RCSB], [https://www.ebi.ac.uk/pdbsum/6ljq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LEG16_HUMAN LEG16_HUMAN]] Binds lactose with high affinity. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>	+	[https://www.uniprot.org/uniprot/LEG16_HUMAN LEG16_HUMAN] Binds lactose with high affinity. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6ljq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6ljq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galectin 3D structures\|Galectin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Su, J]]	+	[[Category: Su J]]
-	~~[[Category: Human galectin-16 r55n]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 20:41, 28 October 2020

OCA — Wed, 28 Oct 2020 20:41:53 GMT

←Older revision		Revision as of 20:41, 28 October 2020
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	==human galectin-16 R55N==		==human galectin-16 R55N==
-	<StructureSection load='6ljq' size='340' side='right'caption='[[6ljq]]' scene=''>	+	<StructureSection load='6ljq' size='340' side='right'caption='[[6ljq]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LJQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ljq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LJQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ljq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljq OCA], [http://pdbe.org/6ljq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ljq RCSB], [http://www.ebi.ac.uk/pdbsum/6ljq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljq ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGALS16 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ljq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljq OCA], [http://pdbe.org/6ljq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ljq RCSB], [http://www.ebi.ac.uk/pdbsum/6ljq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LEG16_HUMAN LEG16_HUMAN]] Binds lactose with high affinity. Strong inducer of T-cell apoptosis.<ref>PMID:19497882</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: The structure of human galectin-16 (Gal-16) has yet to be solved, and its function has remained elusive. METHODS: X-ray crystallography was used to determine the atomic structures of Gal-16 and two of its mutants. The Gal-16 oligomer state was investigated by gel filtration, its hemagglutination activity was determined along with its ability to bind lactose using ITC. The cellular distribution of EGFP-tagged Gal-16 in various cell lines was also investigated, and the interaction between Gal-16 and c-Rel was assessed by pull-down studies, microscale thermophoresis and immunofluorescence. RESULTS: Unlike other galectins, Gal-16 lacks the ability to bind the beta-galactoside lactose. Lactose binding could be regained by replacing an arginine (Arg55) with asparagine, as shown in the crystal structures of two lactose-loaded Gal-16 mutants (R55N and R55N/H57R). Gal-16 was also shown to be monomeric by gel filtration, as well as in crystal structures. Thus, this galectin could not induce erythrocyte agglutination. EGFP-tagged Gal-16 was found to be localized mostly in the nucleus of various cell types, and can interact with c-Rel, a member of NF-kappaB family. CONCLUSIONS: Gal-16 exists as a monomer and its ligand binding is significantly different from that of other prototype galectins, suggesting that it has a novel function(s). The interaction between Gal-16 and c-Rel indicates that Gal-16 may regulate signal transduction pathways via the c-Rel hub in B or T cells at the maternal-fetal interface. GENERAL SIGNIFICANCE: The present study lays the foundation for further studies into the cellular and physiological functions of Gal-16.
		+
		+	Human galectin-16 has a pseudo ligand binding site and plays a role in regulating c-Rel-mediated lymphocyte activity.,Si Y, Yao Y, Jaramillo Ayala G, Li X, Han Q, Zhang W, Xu X, Tai G, Mayo KH, Zhou Y, Su J Biochim Biophys Acta Gen Subj. 2020 Oct 2;1865(1):129755. doi:, 10.1016/j.bbagen.2020.129755. PMID:33011338<ref>PMID:33011338</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ljq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Su J]]	+	[[Category: Su, J]]
		+	[[Category: Human galectin-16 r55n]]
		+	[[Category: Sugar binding protein]]

OCA at 05:44, 14 October 2020

OCA — Wed, 14 Oct 2020 05:44:28 GMT

←Older revision		Revision as of 05:44, 14 October 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6ljq ~~is ON HOLD until Paper Publication~~	+	==human galectin-16 R55N==
-		+	<StructureSection load='6ljq' size='340' side='right'caption='[[6ljq]]' scene=''>
-	~~Authors~~: ~~Su, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LJQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LJQ FirstGlance]. <br>
-	~~Description~~: ~~human galectin~~-~~16 R55N~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ljq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ljq OCA], [http://pdbe.org/6ljq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ljq RCSB], [http://www.ebi.ac.uk/pdbsum/6ljq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ljq ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Su, J]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Su J]]

OCA at 06:33, 1 January 2020

OCA — Wed, 01 Jan 2020 06:33:56 GMT

←Older revision		Revision as of 06:33, 1 January 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ljq is ON HOLD	+	The entry 6ljq is ON HOLD until Paper Publication

	Authors: Su, J.		Authors: Su, J.

OCA: Protected "6ljq" [edit=sysop:move=sysop]

OCA — Wed, 25 Dec 2019 07:06:47 GMT

Protected "6ljq" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:06, 25 December 2019

OCA: New page: '''Unreleased structure''' The entry 6ljq is ON HOLD Authors: Su, J. Description: human galectin-16 R55N Category: Unreleased Structures Category: Su, J

OCA — Wed, 25 Dec 2019 07:06:45 GMT

New page: '''Unreleased structure''' The entry 6ljq is ON HOLD Authors: Su, J. Description: human galectin-16 R55N Category: Unreleased Structures Category: Su, J

New page

'''Unreleased structure'''

The entry 6ljq is ON HOLD

Authors: Su, J.

Description: human galectin-16 R55N
[[Category: Unreleased Structures]]
[[Category: Su, J]]