6lkl - Revision history

OCA at 10:46, 27 March 2024

OCA — Wed, 27 Mar 2024 10:46:43 GMT

←Older revision		Revision as of 10:46, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6lkl' size='340' side='right'caption='[[6lkl]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='6lkl' size='340' side='right'caption='[[6lkl]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lkl]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LKL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lkl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LKL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.213Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>E4U00_07700, EP54_06205, EQ90_12025, HMPREF3211_02751, NCTC10654_00249, NCTC10702_00414, RK64_01575 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1280~~ "~~Micrococcus aureus~~" ~~(Rosenbach 1884) Zopf 1885])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6lkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkl OCA], [~~http~~://pdbe.org/6lkl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lkl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lkl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkl OCA], [https://pdbe.org/6lkl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lkl RCSB], [https://www.ebi.ac.uk/pdbsum/6lkl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkl ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/X5DVD1_STAAU X5DVD1_STAAU]
-	Two-component systems (TCS), which typically consist of a membrane-embedded histidine kinase and a cytoplasmic response regulator, are the dominant signaling proteins for transduction of environmental stimuli into cellular response pathways in prokaryotic cells. HptRSA is a recently identified TCS consisting of the G6P-associated sensor protein (HptA), transmembrane histidine kinase (HptS), and cytoplasmic effector (HptR). HptRSA mediates glucose-6-phosphate (G6P) uptake to support Staphylococcus aureus growth and multiplication within various host cells. How the mechanism by which HptRSA perceives G6P and triggers a downstream response has remained elusive. Here, we solved the HptA structures in apo and G6P-bound states. G6P binding in the cleft between two HptA domains caused a conformational closing movement. The solved structures of HptA in complex with the periplasmic domain of HptS showed that HptA interacts with HptS through both constitutive and switchable interfaces. The G6P-free form of HptA binds to the membrane-distal side of the HptS periplasmic domain (HptSp), resulting in a parallel conformation of the HptSp protomer pair. However, once HptA associates with G6P, its intramolecular domain closure switches the HptA-HptSp contact region into the membrane-proximal domain, which causes rotation and closure of the C termini of each HptSp protomer. Through biochemical and growth assays of HptA and HptS mutant variants, we proposed a distinct mechanism of interface switch-mediated signaling transduction. Our results provide mechanistic insights into bacterial nutrient sensing and expand our understanding of the activation modes by which TCS communicates external signals.	+
-		+
-	~~Interface switch mediates signal transmission in a two-component system.,Wang M, Guo Q, Zhu K, Fang B, Yang Y, Teng M, Li X, Tao Y Proc Natl Acad Sci U S A. 2020 Dec 1;117(48)~~:~~30433-30440. doi:, 10.1073~~/~~pnas.1912080117. Epub 2020 Nov 16. PMID:33199635<ref>PMID:33199635<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6lkl" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Tao, Y~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Wang, M~~]]	+	[[Category: Tao Y]]
-	[[Category: ~~G6p sensor]]~~	+	[[Category: Wang M]]
-	~~[[Category: Signal transduction]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Two-component system~~]]	+

OCA at 12:41, 16 December 2020

OCA — Wed, 16 Dec 2020 12:41:34 GMT

←Older revision		Revision as of 12:41, 16 December 2020
Line 1:		Line 1:

	==Two-component system protein mediate signal transduction==		==Two-component system protein mediate signal transduction==
-	<StructureSection load='6lkl' size='340' side='right'caption='[[6lkl]]' scene=''>	+	<StructureSection load='6lkl' size='340' side='right'caption='[[6lkl]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LKL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lkl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LKL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkl OCA], [http://pdbe.org/6lkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lkl RCSB], [http://www.ebi.ac.uk/pdbsum/6lkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkl ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">E4U00_07700, EP54_06205, EQ90_12025, HMPREF3211_02751, NCTC10654_00249, NCTC10702_00414, RK64_01575 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkl OCA], [http://pdbe.org/6lkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lkl RCSB], [http://www.ebi.ac.uk/pdbsum/6lkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkl ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Two-component systems (TCS), which typically consist of a membrane-embedded histidine kinase and a cytoplasmic response regulator, are the dominant signaling proteins for transduction of environmental stimuli into cellular response pathways in prokaryotic cells. HptRSA is a recently identified TCS consisting of the G6P-associated sensor protein (HptA), transmembrane histidine kinase (HptS), and cytoplasmic effector (HptR). HptRSA mediates glucose-6-phosphate (G6P) uptake to support Staphylococcus aureus growth and multiplication within various host cells. How the mechanism by which HptRSA perceives G6P and triggers a downstream response has remained elusive. Here, we solved the HptA structures in apo and G6P-bound states. G6P binding in the cleft between two HptA domains caused a conformational closing movement. The solved structures of HptA in complex with the periplasmic domain of HptS showed that HptA interacts with HptS through both constitutive and switchable interfaces. The G6P-free form of HptA binds to the membrane-distal side of the HptS periplasmic domain (HptSp), resulting in a parallel conformation of the HptSp protomer pair. However, once HptA associates with G6P, its intramolecular domain closure switches the HptA-HptSp contact region into the membrane-proximal domain, which causes rotation and closure of the C termini of each HptSp protomer. Through biochemical and growth assays of HptA and HptS mutant variants, we proposed a distinct mechanism of interface switch-mediated signaling transduction. Our results provide mechanistic insights into bacterial nutrient sensing and expand our understanding of the activation modes by which TCS communicates external signals.
		+
		+	Interface switch mediates signal transmission in a two-component system.,Wang M, Guo Q, Zhu K, Fang B, Yang Y, Teng M, Li X, Tao Y Proc Natl Acad Sci U S A. 2020 Dec 1;117(48):30433-30440. doi:, 10.1073/pnas.1912080117. Epub 2020 Nov 16. PMID:33199635<ref>PMID:33199635</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6lkl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Tao Y]]	+	[[Category: Tao, Y]]
-	[[Category: Wang M]]	+	[[Category: Wang, M]]
		+	[[Category: G6p sensor]]
		+	[[Category: Signal transduction]]
		+	[[Category: Signaling protein]]
		+	[[Category: Two-component system]]

OCA at 07:43, 2 December 2020

OCA — Wed, 02 Dec 2020 07:43:15 GMT

←Older revision		Revision as of 07:43, 2 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6lkl ~~is ON HOLD~~	+	==Two-component system protein mediate signal transduction==
-		+	<StructureSection load='6lkl' size='340' side='right'caption='[[6lkl]]' scene=''>
-	~~Authors~~: ~~Wang~~, M., ~~Tao~~, Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LKL FirstGlance]. <br>
-	~~Description~~: ~~Two~~-~~component system protein mediate signal transduction~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkl OCA], [http://pdbe.org/6lkl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lkl RCSB], [http://www.ebi.ac.uk/pdbsum/6lkl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Wang, M~~]]	+	__TOC__
-	[[Category: ~~Tao, Y~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Tao Y]]
		+	[[Category: Wang M]]

OCA at 06:34, 1 January 2020

OCA — Wed, 01 Jan 2020 06:34:58 GMT

←Older revision		Revision as of 06:34, 1 January 2020
Line 5:		Line 5:
	Authors: Wang, M., Tao, Y.		Authors: Wang, M., Tao, Y.

-	Description: ~~two~~-component system protein mediate signal transduction	+	Description: Two-component system protein mediate signal transduction
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Wang, M]]		[[Category: Wang, M]]
	[[Category: Tao, Y]]		[[Category: Tao, Y]]

OCA: Protected "6lkl" [edit=sysop:move=sysop]

OCA — Wed, 25 Dec 2019 07:12:18 GMT

Protected "6lkl" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:12, 25 December 2019

OCA: New page: '''Unreleased structure''' The entry 6lkl is ON HOLD Authors: Wang, M., Tao, Y. Description: two-component system protein mediate signal transduction [[Category: Unreleased Structures]...

OCA — Wed, 25 Dec 2019 07:12:16 GMT

New page: '''Unreleased structure''' The entry 6lkl is ON HOLD Authors: Wang, M., Tao, Y. Description: two-component system protein mediate signal transduction [[Category: Unreleased Structures]...

New page

'''Unreleased structure'''

The entry 6lkl is ON HOLD

Authors: Wang, M., Tao, Y.

Description: two-component system protein mediate signal transduction
[[Category: Unreleased Structures]]
[[Category: Wang, M]]
[[Category: Tao, Y]]