6ltd - Revision history

OCA at 14:51, 29 November 2023

OCA — Wed, 29 Nov 2023 14:51:24 GMT

←Older revision		Revision as of 14:51, 29 November 2023
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	<StructureSection load='6ltd' size='340' side='right'caption='[[6ltd]], [[Resolution\|resolution]] 4.10Å' scene=''>		<StructureSection load='6ltd' size='340' side='right'caption='[[6ltd]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ltd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp.~~_sp080513ge~~-23 Streptomyces sp. ~~sp080513ge~~-23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LTD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ltd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._Sp080513GE-23 Streptomyces sp. Sp080513GE-23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LTD FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~, ~~<scene name='pdbligand=EW6:alpha-methyl-L-serine'>EW6</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.1Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~fmoA3 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=630397 Streptomyces sp. Sp080513GE~~-~~23])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=EW6:alpha-methyl-L-serine'>EW6</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ltd OCA], [https://pdbe.org/6ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ltd RCSB], [https://www.ebi.ac.uk/pdbsum/6ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ltd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ltd OCA], [https://pdbe.org/6ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ltd RCSB], [https://www.ebi.ac.uk/pdbsum/6ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ltd ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A077JG85_9ACTN A0A077JG85_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Streptomyces sp. ~~sp080513ge~~-23]]	+	[[Category: Streptomyces sp. Sp080513GE-23]]
-	[[Category: Harada, A]]	+	[[Category: Harada A]]
-	[[Category: Senda, T]]	+	[[Category: Senda T]]
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Jbir-34 and -35~~]]	+

OCA at 07:01, 7 April 2021

OCA — Wed, 07 Apr 2021 07:01:22 GMT

←Older revision		Revision as of 07:01, 7 April 2021
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	==Crystal Structure of Nonribosomal peptide synthetases (NRPS), FmoA3 (S1046A)-alpha-methyl-L-serine-AMP bound form==		==Crystal Structure of Nonribosomal peptide synthetases (NRPS), FmoA3 (S1046A)-alpha-methyl-L-serine-AMP bound form==
-	<StructureSection load='6ltd' size='340' side='right'caption='[[6ltd]]' scene=''>	+	<StructureSection load='6ltd' size='340' side='right'caption='[[6ltd]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LTD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ltd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._sp080513ge-23 Streptomyces sp. sp080513ge-23]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LTD FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ltd OCA], [https://pdbe.org/6ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ltd RCSB], [https://www.ebi.ac.uk/pdbsum/6ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ltd ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=EW6:alpha-methyl-L-serine'>EW6</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fmoA3 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630397 Streptomyces sp. Sp080513GE-23])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ltd OCA], [https://pdbe.org/6ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ltd RCSB], [https://www.ebi.ac.uk/pdbsum/6ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ltd ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nonribosomal peptide synthetases (NRPSs) are attractive targets for bioengineering to generate useful peptides . F moA3 is a single modular NRPS composed of heterocyclization (Cy), adenylation (A), and peptidyl carrier protein (PCP) domains. It use s alpha - methyl- l -serine to synthesize a 4-methyloxazoline ring, probably with another Cy domain in the preceding module FmoA2. Here, we determined the head-to-tail homodimeric structures of FmoA3 by X-ray crystallography ( apo -form, with adenylyl-imidodiphosphate and alpha -methyl- l -seryl-AMP) and cryogenic electron microscopy single particle analysis, and performed site-directed mutagenesis experiments. The data revealed that alpha -methyl- l -serine can be accommodated in the active site because of the extra space around Ala688. The Cy domains of FmoA2 and FmoA3 catalyze peptide bond formation and heterocyclization, respectively. FmoA3's Cy domain seems to lose its donor PCP binding activit y. The collective data support a proposed catalytic cycle of FmoA3.
		+
		+	Structural and functional analyses of the tridomain-nonribosomal peptide synthetase FmoA3 for 4-methyloxazoline ring formation.,Katsuyama Y, Sone K, Harada A, Kawai S, Urano N, Adachi N, Moriya T, Kawasaki M, Shin-Ya K, Senda T, Ohnishi Y Angew Chem Int Ed Engl. 2021 Mar 29. doi: 10.1002/anie.202102760. PMID:33783097<ref>PMID:33783097</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ltd" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Harada A]]	+	[[Category: Streptomyces sp. sp080513ge-23]]
-	[[Category: Senda T]]	+	[[Category: Harada, A]]
		+	[[Category: Senda, T]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Jbir-34 and -35]]

OCA at 17:12, 10 March 2021

OCA — Wed, 10 Mar 2021 17:12:54 GMT

←Older revision		Revision as of 17:12, 10 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6ltd ~~is ON HOLD~~	+	==Crystal Structure of Nonribosomal peptide synthetases (NRPS), FmoA3 (S1046A)-alpha-methyl-L-serine-AMP bound form==
-		+	<StructureSection load='6ltd' size='340' side='right'caption='[[6ltd]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LTD FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ltd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ltd OCA], [https://pdbe.org/6ltd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ltd RCSB], [https://www.ebi.ac.uk/pdbsum/6ltd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ltd ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Harada A]]
		+	[[Category: Senda T]]

OCA at 13:03, 3 March 2021

OCA — Wed, 03 Mar 2021 13:03:53 GMT

←Older revision		Revision as of 13:03, 3 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ltd is ON HOLD ~~until Feb 24 2022~~	+	The entry 6ltd is ON HOLD

	Authors:		Authors:

OCA at 08:30, 17 February 2021

OCA — Wed, 17 Feb 2021 08:30:18 GMT

←Older revision		Revision as of 08:30, 17 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ltd is ON HOLD	+	The entry 6ltd is ON HOLD until Feb 24 2022

	Authors:		Authors:

OCA: Protected "6ltd" [edit=sysop:move=sysop]

OCA — Wed, 29 Jan 2020 13:07:10 GMT

Protected "6ltd" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:07, 29 January 2020

OCA: New page: '''Unreleased structure''' The entry 6ltd is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Jan 2020 13:07:08 GMT

New page: '''Unreleased structure''' The entry 6ltd is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ltd is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]