6lxs - Revision history

OCA at 10:47, 27 March 2024

OCA — Wed, 27 Mar 2024 10:47:51 GMT

←Older revision		Revision as of 10:47, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6lxs' size='340' side='right'caption='[[6lxs]], [[Resolution\|resolution]] 1.58Å' scene=''>		<StructureSection load='6lxs' size='340' side='right'caption='[[6lxs]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6lxs]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LXS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LXS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lxs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LXS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.58Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~sdrC ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6lxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lxs OCA], [~~http~~://pdbe.org/6lxs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lxs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lxs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lxs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lxs OCA], [https://pdbe.org/6lxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lxs RCSB], [https://www.ebi.ac.uk/pdbsum/6lxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lxs ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style="background-color:#fffaf0;">~~	+	== Function ==
-	== ~~Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/SDRC_STAA8 SDRC_STAA8] Cell surface-associated calcium-binding protein which plays an important role in adhesion and pathogenesis. Mediates interactions with components of the extracellular matrix such as host NRXN1 to promote bacterial adhesion.[UniProtKB:O86487]
-	~~Staphylococcus aureus surface proteins play important roles in host tissue colonization, biofilm formation, and bacterial virulence and are thus essential for successful host infections~~. ~~The~~ surface protein SdrC from S. aureus induces bacterial biofilm formation via an intermolecular homophilic interaction of its N2 domains. However, the molecular mechanism of how the homophilic interaction is achieved is unknown. Here, we report two crystal structures of SdrC N2N3 domains, revealing two possible homophilic interaction mechanisms: Ca(2+)-mediated intermolecular metal chelation of N2 domains and intermolecular interaction of N2 and N3 domains. Given the unnecessary role of the N3 domain in the induction of biofilm formation, the N2 domain-~~mediated metal chelation mechanism is likely the mechanism that facilitates SdrC homophilic interaction. Mutation of key Ca(2+)-chelating residues differentially reduced the level of~~ protein ~~dimer formation, further supporting the key~~ role ~~of metal chelation~~ in ~~the N2 domain interaction. Together, these results reveal the possible mechanism of the homophilic interaction of SdrC N2 domains~~ and ~~pave the way for the rational development of new strategies against this mechanism~~.	+
-		+
-	Structural Basis of Staphylococcus aureus Surface Protein SdrC.,Pi Y, Chen W, Ji Q Biochemistry. 2020 Apr 21;59(15):1465-1469. doi: 10.1021/acs.biochem.0c00124., Epub 2020 Apr 7. PMID:32250096<ref>PMID:32250096</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database~~ of the U.~~S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6lxs" style="background-color~~:~~#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ji, Q]]
-	[[Category: Pi, Y]]
-	[[Category: Biofilm accumulation]]
-	[[Category: N2n3 domain]]
-	[[Category: Self-association]]
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: ~~Surface protein~~]]	+	[[Category: Ji Q]]
-	[[Category: ~~Surfactant protein~~]]	+	[[Category: Pi Y]]

OCA at 06:27, 6 May 2020

OCA — Wed, 06 May 2020 06:27:37 GMT

←Older revision		Revision as of 06:27, 6 May 2020
Line 1:		Line 1:

	==CWA protein-SdrC==		==CWA protein-SdrC==
-	<StructureSection load='6lxs' size='340' side='right'caption='[[6lxs]]' scene=''>	+	<StructureSection load='6lxs' size='340' side='right'caption='[[6lxs]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LXS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LXS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lxs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LXS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LXS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lxs OCA], [http://pdbe.org/6lxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lxs RCSB], [http://www.ebi.ac.uk/pdbsum/6lxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lxs ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sdrC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lxs OCA], [http://pdbe.org/6lxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lxs RCSB], [http://www.ebi.ac.uk/pdbsum/6lxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lxs ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus surface proteins play important roles in host tissue colonization, biofilm formation, and bacterial virulence and are thus essential for successful host infections. The surface protein SdrC from S. aureus induces bacterial biofilm formation via an intermolecular homophilic interaction of its N2 domains. However, the molecular mechanism of how the homophilic interaction is achieved is unknown. Here, we report two crystal structures of SdrC N2N3 domains, revealing two possible homophilic interaction mechanisms: Ca(2+)-mediated intermolecular metal chelation of N2 domains and intermolecular interaction of N2 and N3 domains. Given the unnecessary role of the N3 domain in the induction of biofilm formation, the N2 domain-mediated metal chelation mechanism is likely the mechanism that facilitates SdrC homophilic interaction. Mutation of key Ca(2+)-chelating residues differentially reduced the level of protein dimer formation, further supporting the key role of metal chelation in the N2 domain interaction. Together, these results reveal the possible mechanism of the homophilic interaction of SdrC N2 domains and pave the way for the rational development of new strategies against this mechanism.
		+
		+	Structural Basis of Staphylococcus aureus Surface Protein SdrC.,Pi Y, Chen W, Ji Q Biochemistry. 2020 Apr 21;59(15):1465-1469. doi: 10.1021/acs.biochem.0c00124., Epub 2020 Apr 7. PMID:32250096<ref>PMID:32250096</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6lxs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ji Q]]	+	[[Category: Ji, Q]]
-	[[Category: Pi Y]]	+	[[Category: Pi, Y]]
		+	[[Category: Biofilm accumulation]]
		+	[[Category: N2n3 domain]]
		+	[[Category: Self-association]]
		+	[[Category: Staphylococcus aureus]]
		+	[[Category: Surface protein]]
		+	[[Category: Surfactant protein]]

OCA at 05:40, 22 April 2020

OCA — Wed, 22 Apr 2020 05:40:45 GMT

←Older revision		Revision as of 05:40, 22 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6lxs ~~is ON HOLD until Paper Publication~~	+	==CWA protein-SdrC==
-		+	<StructureSection load='6lxs' size='340' side='right'caption='[[6lxs]]' scene=''>
-	~~Authors~~: Pi, Y., Ji, Q.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LXS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LXS FirstGlance]. <br>
-	~~Description~~: ~~CWA protein~~-~~SdrC~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lxs OCA], [http://pdbe.org/6lxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lxs RCSB], [http://www.ebi.ac.uk/pdbsum/6lxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lxs ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Ji, Q]]	+	__TOC__
-	[[Category: Pi, Y]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ji Q]]
		+	[[Category: Pi Y]]

OCA at 05:27, 8 April 2020

OCA — Wed, 08 Apr 2020 05:27:50 GMT

←Older revision		Revision as of 05:27, 8 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6lxs is ON HOLD	+	The entry 6lxs is ON HOLD until Paper Publication

	Authors: Pi, Y., Ji, Q.		Authors: Pi, Y., Ji, Q.

OCA: Protected "6lxs" [edit=sysop:move=sysop]

OCA — Wed, 19 Feb 2020 05:11:17 GMT

Protected "6lxs" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 19 February 2020

OCA: New page: '''Unreleased structure''' The entry 6lxs is ON HOLD Authors: Pi, Y., Ji, Q. Description: CWA protein-SdrC Category: Unreleased Structures Category: Ji, Q Category: Pi, Y

OCA — Wed, 19 Feb 2020 05:11:16 GMT

New page: '''Unreleased structure''' The entry 6lxs is ON HOLD Authors: Pi, Y., Ji, Q. Description: CWA protein-SdrC Category: Unreleased Structures Category: Ji, Q Category: Pi, Y

New page

'''Unreleased structure'''

The entry 6lxs is ON HOLD

Authors: Pi, Y., Ji, Q.

Description: CWA protein-SdrC
[[Category: Unreleased Structures]]
[[Category: Ji, Q]]
[[Category: Pi, Y]]