OCA at 15:05, 29 November 2023

OCA — Wed, 29 Nov 2023 15:05:16 GMT

←Older revision		Revision as of 15:05, 29 November 2023
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	==The crystal structure of SAUGI/KSHVUDG complex==		==The crystal structure of SAUGI/KSHVUDG complex==
-	<StructureSection load='6lyv' size='340' side='right'caption='[[6lyv]]' scene=''>	+	<StructureSection load='6lyv' size='340' side='right'caption='[[6lyv]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LYV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6LYV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6lyv]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_gammaherpesvirus_8 Human gammaherpesvirus 8] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LYV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6lyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lyv OCA], [~~http~~://pdbe.org/6lyv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6lyv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6lyv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6lyv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lyv OCA], [https://pdbe.org/6lyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lyv RCSB], [https://www.ebi.ac.uk/pdbsum/6lyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lyv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/UNG_HHV8P UNG_HHV8P] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or deamination of cytosines. Therefore may reduce deleterious uracil incorporation into the viral genome, particularly in terminally differentiated cells which lack DNA repair enzymes.[HAMAP-Rule:MF_04046]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Uracil-DNA glycosylases (UDGs) are conserved DNA-repair enzymes that can be found in many species, including herpesviruses. Since they play crucial roles for efficient viral DNA replication in herpesviruses, they have been considered as potential antiviral targets. In our previous work, Staphylococcus aureus SAUGI was identified as a DNA mimic protein that targets UDGs from S. aureus, human, Herpes simplex virus (HSV) and Epstein-Barr virus (EBV). Interestingly, SAUGI has the strongest inhibitory effects with EBVUDG. Here, we determined complex structures of SAUGI with EBVUDG and another gamma-herpesvirus UDG from Kaposi's sarcoma-associated herpesvirus (KSHVUDG), which SAUGI fails to effectively inhibit. Structural analysis of the SAUGI/EBVUDG complex suggests that the additional interaction between SAUGI and the leucine loop may explain why SAUGI shows the highest binding capacity with EBVUDG. In contrast, SAUGI appears to make only partial contacts with the key components responsible for the compression and stabilization of the DNA backbone in the leucine loop extension of KSHVUDG. The findings in this study provide a molecular explanation for the differential inhibitory effects and binding strengths that SAUGI has on these two UDGs, and the structural basis of the differences should be helpful in developing inhibitors that would interfere with viral DNA replication.
		+
		+	Structural insight into the differential interactions between the DNA mimic protein SAUGI and two gamma herpesvirus uracil-DNA glycosylases.,Liao YT, Lin SJ, Ko TP, Liu CY, Hsu KC, Wang HC Int J Biol Macromol. 2020 Jun 3;160:903-914. doi: 10.1016/j.ijbiomac.2020.05.267. PMID:32502608<ref>PMID:32502608</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6lyv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA glycosylase 3D structures\|DNA glycosylase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human gammaherpesvirus 8]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Staphylococcus aureus]]
	[[Category: Ko TP]]		[[Category: Ko TP]]
	[[Category: Liao YT]]		[[Category: Liao YT]]
	[[Category: Wang HC]]		[[Category: Wang HC]]

OCA at 06:17, 25 June 2020

OCA — Thu, 25 Jun 2020 06:17:04 GMT

←Older revision		Revision as of 06:17, 25 June 2020
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-	'''Unreleased structure'''

-	The ~~entry~~ 6lyv is ~~ON HOLD until Paper Publication~~	+	==The crystal structure of SAUGI/KSHVUDG complex==
-		+	<StructureSection load='6lyv' size='340' side='right'caption='[[6lyv]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LYV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LYV FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lyv OCA], [http://pdbe.org/6lyv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lyv RCSB], [http://www.ebi.ac.uk/pdbsum/6lyv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lyv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ko TP]]
		+	[[Category: Liao YT]]
		+	[[Category: Wang HC]]

OCA at 05:26, 4 March 2020

OCA — Wed, 04 Mar 2020 05:26:41 GMT

←Older revision		Revision as of 05:26, 4 March 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6lyv is ON HOLD	+	The entry 6lyv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6lyv" [edit=sysop:move=sysop]

OCA — Wed, 26 Feb 2020 08:00:52 GMT

Protected "6lyv" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:00, 26 February 2020

OCA: New page: '''Unreleased structure''' The entry 6lyv is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Feb 2020 08:00:51 GMT

New page: '''Unreleased structure''' The entry 6lyv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6lyv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6lyv - Revision history

OCA at 15:05, 29 November 2023

OCA at 06:17, 25 June 2020

OCA at 05:26, 4 March 2020

OCA: Protected "6lyv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6lyv is ON HOLD Authors: Description: Category: Unreleased Structures