OCA at 15:14, 29 November 2023

OCA — Wed, 29 Nov 2023 15:14:40 GMT

←Older revision		Revision as of 15:14, 29 November 2023
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	==Crystal structure of AnkG/beta4-spectrin complex==		==Crystal structure of AnkG/beta4-spectrin complex==
-	<StructureSection load='6m3r' size='340' side='right'caption='[[6m3r]]' scene=''>	+	<StructureSection load='6m3r' size='340' side='right'caption='[[6m3r]], [[Resolution\|resolution]] 4.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M3R OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6M3R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6m3r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M3R FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6m3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m3r OCA], [~~http~~://pdbe.org/6m3r PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6m3r RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6m3r PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6m3r ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.313Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m3r OCA], [https://pdbe.org/6m3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m3r RCSB], [https://www.ebi.ac.uk/pdbsum/6m3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m3r ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ANK3_RAT ANK3_RAT] Membrane-cytoskeleton linker. May participate in the maintenance/targeting of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments. In skeletal muscle, required for costamere localization of DMD and betaDAG1 (By similarity). Regulates KCNA1 channel activity in function of dietary Mg(2+) levels, and thereby contributes to the regulation of renal Mg(2+) reabsorption (By similarity).[UniProtKB:G5E8K5]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ankyrins (encoded by ANK1/2/3 corresponding to Ankyrin-R/B/G or AnkR/B/G), via binding to spectrins, connect plasma membranes with actin cytoskeleton to maintain mechanical strengths and to modulate excitabilities of diverse cells such as neurons, muscle cells, and erythrocytes. Cellular and genetic evidences suggest that each isoform of ankyrins pairs with a specific beta-spectrin in discrete subcellular membrane microdomains for distinct functions, though the molecular mechanisms underlying such ankyrin/beta-spectrin pairings are unknown. In this study, we discover that a conserved and short extension N-terminal to the ZU5N-ZU5C-UPA tandem (exZZU) is critical for each ankyrin to bind to beta-spectrins with high affinities. Structures of AnkB/G exZZU in complex with spectrin repeats13-15 of beta2/beta4-spectrins solved here reveal that the extension sequence of exZZU forms an additional beta-strand contributing to the structural stability and enhanced affinity of each ZU5N/spectrin repeat interaction. The complex structures further reveal that the UPA domain of exZZU directly participates in spectrin binding. Formation of the exZZU supramodule juxtaposes the ZU5N and UPA domains for simultaneous interacting with spectrin repeats 14 and 15. However, our biochemical and structural investigations indicate that the direct and strong interactions between ankyrins and beta-spectrins do not appear to determine their pairing specificities. Therefore, there likely exists additional mechanism(s) for modulating functional pairings between ankyrins and beta-spectrins in cells.
		+
		+	Structural Basis Underlying Strong Interactions between Ankyrins and Spectrins.,Li J, Chen K, Zhu R, Zhang M J Mol Biol. 2020 Apr 27. pii: S0022-2836(20)30321-1. doi:, 10.1016/j.jmb.2020.04.023. PMID:32353364<ref>PMID:32353364</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6m3r" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ankyrin 3D structures\|Ankyrin 3D structures]]
		+	*[[Spectrin 3D structures\|Spectrin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Rattus norvegicus]]
	[[Category: Chen K]]		[[Category: Chen K]]
	[[Category: Li J]]		[[Category: Li J]]
	[[Category: Zhang M]]		[[Category: Zhang M]]
	[[Category: Zhu R]]		[[Category: Zhu R]]

OCA at 05:26, 13 May 2020

OCA — Wed, 13 May 2020 05:26:24 GMT

←Older revision		Revision as of 05:26, 13 May 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6m3r is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of AnkG/beta4-spectrin complex==
-		+	<StructureSection load='6m3r' size='340' side='right'caption='[[6m3r]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M3R OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M3R FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m3r OCA], [http://pdbe.org/6m3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m3r RCSB], [http://www.ebi.ac.uk/pdbsum/6m3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m3r ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chen K]]
		+	[[Category: Li J]]
		+	[[Category: Zhang M]]
		+	[[Category: Zhu R]]

OCA at 08:17, 18 March 2020

OCA — Wed, 18 Mar 2020 08:17:02 GMT

←Older revision		Revision as of 08:17, 18 March 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6m3r is ON HOLD	+	The entry 6m3r is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6m3r" [edit=sysop:move=sysop]

OCA — Wed, 11 Mar 2020 05:59:38 GMT

Protected "6m3r" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:59, 11 March 2020

OCA: New page: '''Unreleased structure''' The entry 6m3r is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 11 Mar 2020 05:59:36 GMT

New page: '''Unreleased structure''' The entry 6m3r is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6m3r is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6m3r - Revision history

OCA at 15:14, 29 November 2023

OCA at 05:26, 13 May 2020

OCA at 08:17, 18 March 2020

OCA: Protected "6m3r" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6m3r is ON HOLD Authors: Description: Category: Unreleased Structures