6m6s - Revision history

OCA at 15:19, 29 November 2023

OCA — Wed, 29 Nov 2023 15:19:13 GMT

←Older revision		Revision as of 15:19, 29 November 2023
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-	====	+	==Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5'-ppp 12-mer dsRNA==
-	<StructureSection load='6m6s' size='340' side='right'caption='[[6m6s]]' scene=''>	+	<StructureSection load='6m6s' size='340' side='right'caption='[[6m6s]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6m6s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M6S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m6s OCA], [https://pdbe.org/6m6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m6s RCSB], [https://www.ebi.ac.uk/pdbsum/6m6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m6s ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m6s OCA], [https://pdbe.org/6m6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m6s RCSB], [https://www.ebi.ac.uk/pdbsum/6m6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m6s ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q93413_CAEEL Q93413_CAEEL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DRH-3 is critically involved in germline development and RNA interference (RNAi) facilitated chromosome segregation via the 22G-siRNA pathway in Caenorhabditis elegans. DRH-3 has similar domain architecture to RIG-I-like receptors (RLRs) and belongs to the RIG-I-like RNA helicase family. The molecular understanding of DRH-3 and its function in endogenous RNAi pathways remains elusive. In this study, we solved the crystal structures of the DRH-3 N-terminal domain (NTD) and the C-terminal domains (CTDs) in complex with 5'-triphosphorylated RNAs. The NTD of DRH-3 adopts a distinct fold of tandem caspase activation and recruitment domains (CARDs) structurally similar to the CARDs of RIG-I and MDA5, suggesting a signaling function in the endogenous RNAi biogenesis. The CTD preferentially recognizes 5'-triphosphorylated double-stranded RNAs bearing the typical features of secondary siRNA transcripts. The full-length DRH-3 displays unique structural dynamics upon binding to RNA duplexes that differ from RIG-I or MDA5. These features of DRH-3 showcase the evolutionary divergence of the Dicer and RLR family of helicases.
		+
		+	Insights into the structure and RNA-binding specificity of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3).,Li K, Zheng J, Wirawan M, Trinh NM, Fedorova O, Griffin PR, Pyle AM, Luo D Nucleic Acids Res. 2021 Sep 27;49(17):9978-9991. doi: 10.1093/nar/gkab712. PMID:34403472<ref>PMID:34403472</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6m6s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Caenorhabditis elegans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z~~-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Fedorova O]]
		+	[[Category: Griffin P]]
		+	[[Category: Li K]]
		+	[[Category: Luo D]]
		+	[[Category: Plyle A]]
		+	[[Category: Wirawan M]]
		+	[[Category: Xiong Z]]
		+	[[Category: Zheng J]]

OCA at 06:41, 17 March 2021

OCA — Wed, 17 Mar 2021 06:41:33 GMT

←Older revision		Revision as of 06:41, 17 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6m6s ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6m6s' size='340' side='right'caption='[[6m6s]]' scene=''>
-	~~Authors~~: ~~Li, K~~.~~, Zheng, J~~.~~, Wirawan, M~~.~~, Xiong, Z~~.~~, Fedorova, O~~.~~, Griffin, P~~.~~, Plyle, A., Luo, D~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5~~'~~-ppp 12-mer dsRNA~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m6s OCA], [https://pdbe.org/6m6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m6s RCSB], [https://www.ebi.ac.uk/pdbsum/6m6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m6s ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Wirawan~~, ~~M]]~~	+	__TOC__
-	[~~[Category~~: Li, ~~K]]~~	+	</StructureSection>
-	[~~[Category~~: ~~Griffin, P~~]]	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Plyle~~, ~~A]]~~	+	[[Category: Z-disk]]
-	[~~[Category~~: ~~Zheng, J]~~]	+
-	[[Category: ~~Luo, D~~]]	+
-	[[Category: ~~Xiong,~~ Z]]	+
-	~~[[Category: Fedorova, O~~]]	+

OCA at 05:06, 3 June 2020

OCA — Wed, 03 Jun 2020 05:06:29 GMT

←Older revision		Revision as of 05:06, 3 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6m6s is ON HOLD	+	The entry 6m6s is ON HOLD until Paper Publication

	Authors: Li, K., Zheng, J., Wirawan, M., Xiong, Z., Fedorova, O., Griffin, P., Plyle, A., Luo, D.		Authors: Li, K., Zheng, J., Wirawan, M., Xiong, Z., Fedorova, O., Griffin, P., Plyle, A., Luo, D.
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	[[Category: Wirawan, M]]		[[Category: Wirawan, M]]
	[[Category: Li, K]]		[[Category: Li, K]]
-	[[Category: Fedorova, O]]
	[[Category: Griffin, P]]		[[Category: Griffin, P]]
	[[Category: Plyle, A]]		[[Category: Plyle, A]]
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	[[Category: Luo, D]]		[[Category: Luo, D]]
	[[Category: Xiong, Z]]		[[Category: Xiong, Z]]
		+	[[Category: Fedorova, O]]

OCA: Protected "6m6s" [edit=sysop:move=sysop]

OCA — Fri, 27 Mar 2020 08:17:27 GMT

Protected "6m6s" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:17, 27 March 2020

OCA: New page: '''Unreleased structure''' The entry 6m6s is ON HOLD Authors: Li, K., Zheng, J., Wirawan, M., Xiong, Z., Fedorova, O., Griffin, P., Plyle, A., Luo, D. Description: Crystal structure of...

OCA — Fri, 27 Mar 2020 08:17:25 GMT

New page: '''Unreleased structure''' The entry 6m6s is ON HOLD Authors: Li, K., Zheng, J., Wirawan, M., Xiong, Z., Fedorova, O., Griffin, P., Plyle, A., Luo, D. Description: Crystal structure of...

New page

'''Unreleased structure'''

The entry 6m6s is ON HOLD

Authors: Li, K., Zheng, J., Wirawan, M., Xiong, Z., Fedorova, O., Griffin, P., Plyle, A., Luo, D.

Description: Crystal structure of Caenorhabditis elegans Dicer-related helicase 3 (DRH-3) C-terminal domain with 5'-ppp 12-mer dsRNA
[[Category: Unreleased Structures]]
[[Category: Wirawan, M]]
[[Category: Li, K]]
[[Category: Fedorova, O]]
[[Category: Griffin, P]]
[[Category: Plyle, A]]
[[Category: Zheng, J]]
[[Category: Luo, D]]
[[Category: Xiong, Z]]