6m9i - Revision history

OCA at 14:41, 13 March 2024

OCA — Wed, 13 Mar 2024 14:41:51 GMT

←Older revision		Revision as of 14:41, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6m9i' size='340' side='right'caption='[[6m9i]], [[Resolution\|resolution]] 0.90Å' scene=''>		<StructureSection load='6m9i' size='340' side='right'caption='[[6m9i]], [[Resolution\|resolution]] 0.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6m9i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M9I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6M9I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6m9i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae_pv._syringae Pseudomonas syringae pv. syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M9I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M9I FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[[~~6eex~~\|~~6eex~~]]</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution\|Resolution]] 0.902Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6m9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m9i OCA], [~~http~~://pdbe.org/6m9i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6m9i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6m9i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6m9i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m9i OCA], [https://pdbe.org/6m9i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m9i RCSB], [https://www.ebi.ac.uk/pdbsum/6m9i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m9i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ICEN_PSESY ICEN_PSESY]] Ice nucleation proteins enable bacteria to nucleate crystallization in supercooled water.	+	[https://www.uniprot.org/uniprot/ICEN_PSESY ICEN_PSESY] Ice nucleation proteins enable bacteria to nucleate crystallization in supercooled water.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The ice-nucleation protein InaZ from Pseudomonas syringae contains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA. Ab initio structures of this repeat segment, resolved to 1.1 A by microfocus X-ray crystallography and to 0.9 A by the cryo-EM method MicroED, were determined from both racemic and homochiral crystals. The benefits of racemic protein crystals for structure determination by MicroED were evaluated and it was confirmed that the phase restriction introduced by crystal centrosymmetry increases the number of successful trials during the ab initio phasing of the electron diffraction data. Both homochiral and racemic GSTSTA form amyloid-like protofibrils with labile, corrugated antiparallel beta-sheets that mate face to back. The racemic GSTSTA protofibril represents a new class of amyloid assembly in which all-left-handed sheets mate with their all-right-handed counterparts. This determination of racemic amyloid assemblies by MicroED reveals complex amyloid architectures and illustrates the racemic advantage in macromolecular crystallography, now with submicrometre-sized crystals.	+
-		+
-	Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ.,Zee CT, Glynn C, Gallagher-Jones M, Miao J, Santiago CG, Cascio D, Gonen T, Sawaya MR, Rodriguez JA IUCrJ. 2019 Jan 24;6(Pt 2):197-205. doi: 10.1107/S2052252518017621. eCollection, 2019 Mar 1. PMID:30867917<ref>PMID:30867917</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6m9i" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cascio, D]]	+	[[Category: Pseudomonas syringae pv. syringae]]
-	[[Category: Gallagher-Jones, M]]	+	[[Category: Cascio D]]
-	[[Category: Glynn, C]]	+	[[Category: Gallagher-Jones M]]
-	[[Category: Gonen, T]]	+	[[Category: Glynn C]]
-	[[Category: Miao, J]]	+	[[Category: Gonen T]]
-	[[Category: Rodriguez~~, J A~~]]	+	[[Category: Miao J]]
-	[[Category: Santiago~~, C G~~]]	+	[[Category: Rodriguez JA]]
-	[[Category: Sawaya~~, M R~~]]	+	[[Category: Santiago CG]]
-	[[Category: Zee, C]]	+	[[Category: Sawaya MR]]
-	~~[[Category: Amyloid]]~~	+	[[Category: Zee C]]
-	~~[[Category: Ice nucleation]]~~	+
-	~~[[Category: Inaz]]~~	+
-	~~[[Category: Microed]]~~	+
-	~~[[Category: Protein fibril]]~~	+
-	~~[[Category: Pseudomonas syringae]]~~	+
-	~~[[Category: Racemic~~]]	+

OCA at 06:41, 27 March 2019

OCA — Wed, 27 Mar 2019 06:41:29 GMT

←Older revision		Revision as of 06:41, 27 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6m9i is ~~ON HOLD until Paper~~ Publication	+	==L-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712==
		+	<StructureSection load='6m9i' size='340' side='right'caption='[[6m9i]], [[Resolution\|resolution]] 0.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6m9i]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M9I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6M9I FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6eex\|6eex]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6m9i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m9i OCA], [http://pdbe.org/6m9i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m9i RCSB], [http://www.ebi.ac.uk/pdbsum/6m9i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m9i ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ICEN_PSESY ICEN_PSESY]] Ice nucleation proteins enable bacteria to nucleate crystallization in supercooled water.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ice-nucleation protein InaZ from Pseudomonas syringae contains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA. Ab initio structures of this repeat segment, resolved to 1.1 A by microfocus X-ray crystallography and to 0.9 A by the cryo-EM method MicroED, were determined from both racemic and homochiral crystals. The benefits of racemic protein crystals for structure determination by MicroED were evaluated and it was confirmed that the phase restriction introduced by crystal centrosymmetry increases the number of successful trials during the ab initio phasing of the electron diffraction data. Both homochiral and racemic GSTSTA form amyloid-like protofibrils with labile, corrugated antiparallel beta-sheets that mate face to back. The racemic GSTSTA protofibril represents a new class of amyloid assembly in which all-left-handed sheets mate with their all-right-handed counterparts. This determination of racemic amyloid assemblies by MicroED reveals complex amyloid architectures and illustrates the racemic advantage in macromolecular crystallography, now with submicrometre-sized crystals.

-	~~Authors:~~ Zee~~, C.~~, Glynn, C., Gallagher-Jones, M., Miao, J., Santiago~~, C.G.~~, Cascio, D., Gonen, T., Sawaya, M.R., ~~Rodriguez, J.A~~.	+	Homochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ.,Zee CT, Glynn C, Gallagher-Jones M, Miao J, Santiago CG, Cascio D, Gonen T, Sawaya MR, Rodriguez JA IUCrJ. 2019 Jan 24;6(Pt 2):197-205. doi: 10.1107/S2052252518017621. eCollection, 2019 Mar 1. PMID:30867917<ref>PMID:30867917</ref>

-	~~Description: L-GSTSTA from degenerate octameric repeats in InaZ~~, ~~residues 707-712~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Gonen, T]]~~	+	<div class="pdbe-citations 6m9i" style="background-color:#fffaf0;"></div>
-	~~[[Category: Rodriguez, J.A]]~~	+	== References ==
-	~~[[Category: Glynn, C]]~~	+	<references/>
-	~~[[Category: Zee, C]]~~	+	__TOC__
-	~~[[Category: Santiago, C.G]]~~	+	</StructureSection>
-	[[Category: ~~Miao, J]]~~	+	[[Category: Large Structures]]
-	~~[[Category: Sawaya, M.R~~]]	+
	[[Category: Cascio, D]]		[[Category: Cascio, D]]
	[[Category: Gallagher-Jones, M]]		[[Category: Gallagher-Jones, M]]
		+	[[Category: Glynn, C]]
		+	[[Category: Gonen, T]]
		+	[[Category: Miao, J]]
		+	[[Category: Rodriguez, J A]]
		+	[[Category: Santiago, C G]]
		+	[[Category: Sawaya, M R]]
		+	[[Category: Zee, C]]
		+	[[Category: Amyloid]]
		+	[[Category: Ice nucleation]]
		+	[[Category: Inaz]]
		+	[[Category: Microed]]
		+	[[Category: Protein fibril]]
		+	[[Category: Pseudomonas syringae]]
		+	[[Category: Racemic]]

OCA at 08:51, 5 September 2018

OCA — Wed, 05 Sep 2018 08:51:00 GMT

←Older revision		Revision as of 08:51, 5 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6m9i is ON HOLD	+	The entry 6m9i is ON HOLD until Paper Publication

	Authors: Zee, C., Glynn, C., Gallagher-Jones, M., Miao, J., Santiago, C.G., Cascio, D., Gonen, T., Sawaya, M.R., Rodriguez, J.A.		Authors: Zee, C., Glynn, C., Gallagher-Jones, M., Miao, J., Santiago, C.G., Cascio, D., Gonen, T., Sawaya, M.R., Rodriguez, J.A.
Line 7:		Line 7:
	Description: L-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712		Description: L-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Zee, C]]
-	[[Category: Miao, J]]
-	[[Category: Sawaya, M.R]]
	[[Category: Gonen, T]]		[[Category: Gonen, T]]
	[[Category: Rodriguez, J.A]]		[[Category: Rodriguez, J.A]]
	[[Category: Glynn, C]]		[[Category: Glynn, C]]
		+	[[Category: Zee, C]]
	[[Category: Santiago, C.G]]		[[Category: Santiago, C.G]]
		+	[[Category: Miao, J]]
		+	[[Category: Sawaya, M.R]]
	[[Category: Cascio, D]]		[[Category: Cascio, D]]
	[[Category: Gallagher-Jones, M]]		[[Category: Gallagher-Jones, M]]

OCA: Protected "6m9i" [edit=sysop:move=sysop]

OCA — Wed, 29 Aug 2018 06:09:36 GMT

Protected "6m9i" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:09, 29 August 2018

OCA: New page: '''Unreleased structure''' The entry 6m9i is ON HOLD Authors: Zee, C., Glynn, C., Gallagher-Jones, M., Miao, J., Santiago, C.G., Cascio, D., Gonen, T., Sawaya, M.R., Rodriguez, J.A. De...

OCA — Wed, 29 Aug 2018 06:09:35 GMT

New page: '''Unreleased structure''' The entry 6m9i is ON HOLD Authors: Zee, C., Glynn, C., Gallagher-Jones, M., Miao, J., Santiago, C.G., Cascio, D., Gonen, T., Sawaya, M.R., Rodriguez, J.A. De...

New page

'''Unreleased structure'''

The entry 6m9i is ON HOLD

Authors: Zee, C., Glynn, C., Gallagher-Jones, M., Miao, J., Santiago, C.G., Cascio, D., Gonen, T., Sawaya, M.R., Rodriguez, J.A.

Description: L-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712
[[Category: Unreleased Structures]]
[[Category: Zee, C]]
[[Category: Miao, J]]
[[Category: Sawaya, M.R]]
[[Category: Gonen, T]]
[[Category: Rodriguez, J.A]]
[[Category: Glynn, C]]
[[Category: Santiago, C.G]]
[[Category: Cascio, D]]
[[Category: Gallagher-Jones, M]]