6mb7 - Revision history

OCA at 06:27, 11 October 2023

OCA — Wed, 11 Oct 2023 06:27:50 GMT

←Older revision		Revision as of 06:27, 11 October 2023
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	==Binary (paromomycin) structure of AAC-IIIb==		==Binary (paromomycin) structure of AAC-IIIb==
-	<StructureSection load='6mb7' size='340' side='right' caption='[[6mb7]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='6mb7' size='340' side='right'caption='[[6mb7]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mb7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MB7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mb7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MB7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=PAR:PAROMOMYCIN'>PAR</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>aac(3)-IIIb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~287~~ "~~Bacillus aeruginosus~~" ~~(Schroeter 1872) Trevisan 1885])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mb7 OCA], [~~http~~://pdbe.org/6mb7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mb7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mb7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mb7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mb7 OCA], [https://pdbe.org/6mb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mb7 RCSB], [https://www.ebi.ac.uk/pdbsum/6mb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mb7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q51405_PSEAI Q51405_PSEAI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cuneo, M J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kumar, P~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Acetyltransferase~~]]	+	[[Category: Cuneo MJ]]
-	[[Category: ~~Antibiotic]]~~	+	[[Category: Kumar P]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Gnat]]~~	+
-	~~[[Category: Promiscuity]]~~	+
-	~~[[Category: Transferase-antibiotic complex~~]]	+

OCA at 07:09, 5 December 2018

OCA — Wed, 05 Dec 2018 07:09:30 GMT

←Older revision		Revision as of 07:09, 5 December 2018
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	<StructureSection load='6mb7' size='340' side='right' caption='[[6mb7]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='6mb7' size='340' side='right' caption='[[6mb7]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mb7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mb7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MB7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac(3)-IIIb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mb7 OCA], [http://pdbe.org/6mb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mb7 RCSB], [http://www.ebi.ac.uk/pdbsum/6mb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mb7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mb7 OCA], [http://pdbe.org/6mb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mb7 RCSB], [http://www.ebi.ac.uk/pdbsum/6mb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mb7 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:24, 7 November 2018

OCA — Wed, 07 Nov 2018 12:24:10 GMT

←Older revision		Revision as of 12:24, 7 November 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6mb7 is ~~ON HOLD until Paper~~ Publication	+	==Binary (paromomycin) structure of AAC-IIIb==
		+	<StructureSection load='6mb7' size='340' side='right' caption='[[6mb7]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mb7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MB7 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mb7 OCA], [http://pdbe.org/6mb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mb7 RCSB], [http://www.ebi.ac.uk/pdbsum/6mb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mb7 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aminoglycoside antibiotics are a large family of antibiotics that can be divided into two distinct classes on the basis of the substitution pattern of the central deoxystreptamine ring. Although aminoglycosides are chemically, structurally, and topologically diverse, some aminoglycoside-modifying enzymes (AGMEs) are able to inactivate as many as 15 aminoglycosides from the two main classes, the kanamycin- and neomycin-based antibiotics. Here, we present the crystal structure of a promiscuous AGME, aminoglycoside- N3-acetyltransferase-IIIb (AAC-IIIb), in the apo form, in binary drug (sisomicin, neomycin, and paromomycin) and coenzyme A (CoASH) complexes, and in the ternary neomycin-CoASH complex. These data provide a structural framework for interpretation of the thermodynamics of enzyme-ligand interactions and the role of solvent in the recognition of ligands. In combination with the recent structure of an AGME that does not have broad substrate specificity, these structures allow for the direct determination of how antibiotic promiscuity is encoded in some AGMEs.

-	~~Authors: Cuneo, M.J~~., Kumar, P.	+	Encoding of Promiscuity in an Aminoglycoside Acetyltransferase.,Kumar P, Selvaraj B, Serpersu EH, Cuneo MJ J Med Chem. 2018 Oct 31. doi: 10.1021/acs.jmedchem.8b01393. PMID:30347146<ref>PMID:30347146</ref>

-	~~Description: Binary (paromomycin) structure~~ of ~~AAC-IIIb~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Cuneo, M.J]]	+	<div class="pdbe-citations 6mb7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cuneo, M J]]
	[[Category: Kumar, P]]		[[Category: Kumar, P]]
		+	[[Category: Acetyltransferase]]
		+	[[Category: Antibiotic]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Gnat]]
		+	[[Category: Promiscuity]]
		+	[[Category: Transferase-antibiotic complex]]

OCA at 06:11, 12 September 2018

OCA — Wed, 12 Sep 2018 06:11:49 GMT

←Older revision		Revision as of 06:11, 12 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mb7 is ON HOLD	+	The entry 6mb7 is ON HOLD until Paper Publication

	Authors: Cuneo, M.J., Kumar, P.		Authors: Cuneo, M.J., Kumar, P.

OCA: Protected "6mb7" [edit=sysop:move=sysop]

OCA — Wed, 05 Sep 2018 08:54:47 GMT

Protected "6mb7" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:54, 5 September 2018

OCA: New page: '''Unreleased structure''' The entry 6mb7 is ON HOLD Authors: Cuneo, M.J., Kumar, P. Description: Binary (paromomycin) structure of AAC-IIIb Category: Unreleased Structures [[Categ...

OCA — Wed, 05 Sep 2018 08:54:46 GMT

New page: '''Unreleased structure''' The entry 6mb7 is ON HOLD Authors: Cuneo, M.J., Kumar, P. Description: Binary (paromomycin) structure of AAC-IIIb Category: Unreleased Structures [[Categ...

New page

'''Unreleased structure'''

The entry 6mb7 is ON HOLD

Authors: Cuneo, M.J., Kumar, P.

Description: Binary (paromomycin) structure of AAC-IIIb
[[Category: Unreleased Structures]]
[[Category: Cuneo, M.J]]
[[Category: Kumar, P]]