6mfz - Revision history

OCA at 06:30, 11 October 2023

OCA — Wed, 11 Oct 2023 06:30:59 GMT

←Older revision		Revision as of 06:30, 11 October 2023
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	<StructureSection load='6mfz' size='340' side='right'caption='[[6mfz]], [[Resolution\|resolution]] 6.00Å' scene=''>		<StructureSection load='6mfz' size='340' side='right'caption='[[6mfz]], [[Resolution\|resolution]] 6.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mfz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_10027 Atcc 10027~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MFZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mfz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacillus_parabrevis Brevibacillus parabrevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MFZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PNS:4~~-~~PHOSPHOPANTETHEINE'>PNS</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~lgrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=54914 ATCC 10027])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mfz OCA], [~~http~~://pdbe.org/6mfz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mfz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mfz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mfz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mfz OCA], [https://pdbe.org/6mfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mfz RCSB], [https://www.ebi.ac.uk/pdbsum/6mfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mfz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LGRA_BREPA LGRA_BREPA]] Activates valine (or leucine, but much less frequently), and then glycine and catalyzes the formation of the peptide bond in the first step of peptide synthesis. This enzyme may also play a role in N-formylation of the first amino acid residue in the synthesized dipeptide.	+	[https://www.uniprot.org/uniprot/LGRA_BREPA LGRA_BREPA] Activates valine (or leucine, but much less frequently), and then glycine and catalyzes the formation of the peptide bond in the first step of peptide synthesis. This enzyme may also play a role in N-formylation of the first amino acid residue in the synthesized dipeptide.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6mfz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6mfz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Linear gramicidin synthase\|Linear gramicidin synthase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 10027~~]]	+	[[Category: Brevibacillus parabrevis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eivaskhani, M]]	+	[[Category: Eivaskhani M]]
-	[[Category: Harb, I]]	+	[[Category: Harb I]]
-	[[Category: Reimer~~, J M~~]]	+	[[Category: Reimer JM]]
-	[[Category: Schmeing~~, T M]]~~	+	[[Category: Schmeing TM]]
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Linear gramicidin]]~~	+
-	~~[[Category: Natural product]]~~	+
-	~~[[Category: Nonribosomal peptide synthetase]]~~	+
-	~~[[Category: Nrp]]~~	+
-	~~[[Category: Tailoring domain~~]]	+

OCA at 09:04, 8 January 2020

OCA — Wed, 08 Jan 2020 09:04:43 GMT

←Older revision		Revision as of 09:04, 8 January 2020
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	<StructureSection load='6mfz' size='340' side='right'caption='[[6mfz]], [[Resolution\|resolution]] 6.00Å' scene=''>		<StructureSection load='6mfz' size='340' side='right'caption='[[6mfz]], [[Resolution\|resolution]] 6.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mfz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MFZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mfz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10027 Atcc 10027]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MFZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lgrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=54914 ATCC 10027])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mfz OCA], [http://pdbe.org/6mfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mfz RCSB], [http://www.ebi.ac.uk/pdbsum/6mfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mfz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mfz OCA], [http://pdbe.org/6mfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mfz RCSB], [http://www.ebi.ac.uk/pdbsum/6mfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mfz ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 10027]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Eivaskhani, M]]		[[Category: Eivaskhani, M]]

OCA at 15:24, 20 November 2019

OCA — Wed, 20 Nov 2019 15:24:33 GMT

←Older revision		Revision as of 15:24, 20 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6mfz is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of dimodular LgrA in a condensation state==
		+	<StructureSection load='6mfz' size='340' side='right'caption='[[6mfz]], [[Resolution\|resolution]] 6.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mfz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MFZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MFZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mfz OCA], [http://pdbe.org/6mfz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mfz RCSB], [http://www.ebi.ac.uk/pdbsum/6mfz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mfz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LGRA_BREPA LGRA_BREPA]] Activates valine (or leucine, but much less frequently), and then glycine and catalyzes the formation of the peptide bond in the first step of peptide synthesis. This enzyme may also play a role in N-formylation of the first amino acid residue in the synthesized dipeptide.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nonribosomal peptide synthetases (NRPSs) are biosynthetic enzymes that synthesize natural product therapeutics using a modular synthetic logic, whereby each module adds one aminoacyl substrate to the nascent peptide. We have determined five x-ray crystal structures of large constructs of the NRPS linear gramicidin synthetase, including a structure of a full core dimodule in conformations organized for the condensation reaction and intermodular peptidyl substrate delivery. The structures reveal differences in the relative positions of adjacent modules, which are not strictly coupled to the catalytic cycle and are consistent with small-angle x-ray scattering data. The structures and covariation analysis of homologs allowed us to create mutants that improve the yield of a peptide from a module-swapped dimodular NRPS.

-	~~Authors~~:	+	Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility.,Reimer JM, Eivaskhani M, Harb I, Guarne A, Weigt M, Schmeing TM Science. 2019 Nov 8;366(6466). pii: 366/6466/eaaw4388. doi:, 10.1126/science.aaw4388. PMID:31699907<ref>PMID:31699907</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6mfz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Eivaskhani, M]]
		+	[[Category: Harb, I]]
		+	[[Category: Reimer, J M]]
		+	[[Category: Schmeing, T M]]
		+	[[Category: Enzyme]]
		+	[[Category: Ligase]]
		+	[[Category: Linear gramicidin]]
		+	[[Category: Natural product]]
		+	[[Category: Nonribosomal peptide synthetase]]
		+	[[Category: Nrp]]
		+	[[Category: Tailoring domain]]

OCA at 04:18, 24 July 2019

OCA — Wed, 24 Jul 2019 04:18:47 GMT

←Older revision		Revision as of 04:18, 24 July 2019
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	The entry 6mfz is ON HOLD until Paper Publication		The entry 6mfz is ON HOLD until Paper Publication

-	Authors: ~~Reimer, J.M., Eivaskhani, M., Harb, I., Schmeing, T.M.~~	+	Authors:

-	Description: ~~Crystal structure of dimodular LgrA in a condensation state~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Eivaskhani, M]]
-	[[Category: Schmeing, T.M]]
-	[[Category: Reimer, J.M]]
-	[[Category: Harb, I]]

OCA at 08:50, 17 July 2019

OCA — Wed, 17 Jul 2019 08:50:55 GMT

←Older revision		Revision as of 08:50, 17 July 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mfz is ON HOLD	+	The entry 6mfz is ON HOLD until Paper Publication

	Authors: Reimer, J.M., Eivaskhani, M., Harb, I., Schmeing, T.M.		Authors: Reimer, J.M., Eivaskhani, M., Harb, I., Schmeing, T.M.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Eivaskhani, M]]		[[Category: Eivaskhani, M]]
		+	[[Category: Schmeing, T.M]]
	[[Category: Reimer, J.M]]		[[Category: Reimer, J.M]]
	[[Category: Harb, I]]		[[Category: Harb, I]]
-	[[Category: Schmeing, T.M]]

OCA at 21:02, 5 June 2019

OCA — Wed, 05 Jun 2019 21:02:43 GMT

←Older revision		Revision as of 21:02, 5 June 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mfz is ON HOLD ~~until Paper Publication~~	+	The entry 6mfz is ON HOLD

-	Authors: Reimer, J.M., Harb, I., Schmeing, T.M.	+	Authors: Reimer, J.M., Eivaskhani, M., Harb, I., Schmeing, T.M.

-	Description: Crystal structure of dimodular LgrA in ~~the substrate donation~~ state	+	Description: Crystal structure of dimodular LgrA in a condensation state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Schmeing~~, T.M]]	+	[[Category: Eivaskhani, M]]
	[[Category: Reimer, J.M]]		[[Category: Reimer, J.M]]
	[[Category: Harb, I]]		[[Category: Harb, I]]
		+	[[Category: Schmeing, T.M]]

OCA at 06:24, 3 October 2018

OCA — Wed, 03 Oct 2018 06:24:30 GMT

←Older revision		Revision as of 06:24, 3 October 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mfz is ON HOLD	+	The entry 6mfz is ON HOLD until Paper Publication

	Authors: Reimer, J.M., Harb, I., Schmeing, T.M.		Authors: Reimer, J.M., Harb, I., Schmeing, T.M.
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	Description: Crystal structure of dimodular LgrA in the substrate donation state		Description: Crystal structure of dimodular LgrA in the substrate donation state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Schmeing, T.M]]
	[[Category: Reimer, J.M]]		[[Category: Reimer, J.M]]
	[[Category: Harb, I]]		[[Category: Harb, I]]
-	[[Category: Schmeing, T.M]]

OCA: Protected "6mfz" [edit=sysop:move=sysop]

OCA — Wed, 19 Sep 2018 18:28:24 GMT

Protected "6mfz" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:28, 19 September 2018

OCA: New page: '''Unreleased structure''' The entry 6mfz is ON HOLD Authors: Reimer, J.M., Harb, I., Schmeing, T.M. Description: Crystal structure of dimodular LgrA in the substrate donation state [[...

OCA — Wed, 19 Sep 2018 18:28:23 GMT

New page: '''Unreleased structure''' The entry 6mfz is ON HOLD Authors: Reimer, J.M., Harb, I., Schmeing, T.M. Description: Crystal structure of dimodular LgrA in the substrate donation state [[...

New page

'''Unreleased structure'''

The entry 6mfz is ON HOLD

Authors: Reimer, J.M., Harb, I., Schmeing, T.M.

Description: Crystal structure of dimodular LgrA in the substrate donation state
[[Category: Unreleased Structures]]
[[Category: Reimer, J.M]]
[[Category: Harb, I]]
[[Category: Schmeing, T.M]]