6mjs - Revision history

OCA at 06:33, 11 October 2023

OCA — Wed, 11 Oct 2023 06:33:09 GMT

←Older revision		Revision as of 06:33, 11 October 2023
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	<StructureSection load='6mjs' size='340' side='right'caption='[[6mjs]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='6mjs' size='340' side='right'caption='[[6mjs]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mjs]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MJS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MJS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mjs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MJS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MJS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=REQ:(1,10+PHENANTHROLINE)-(TRI-CARBON+MONOXIDE)+RHENIUM+(I)'>REQ</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=REQ:(1,10+PHENANTHROLINE)-(TRI-CARBON+MONOXIDE)+RHENIUM+(I)'>REQ</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mjs OCA], [~~http~~://pdbe.org/6mjs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mjs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mjs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mjs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mjs OCA], [https://pdbe.org/6mjs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mjs RCSB], [https://www.ebi.ac.uk/pdbsum/6mjs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mjs ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.	+	[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Gray~~, H B~~]]	+	[[Category: Gray HB]]
-	[[Category: Heyda, J]]	+	[[Category: Heyda J]]
-	[[Category: Hollas, D]]	+	[[Category: Hollas D]]
-	[[Category: Kaiser~~, J T~~]]	+	[[Category: Kaiser JT]]
-	[[Category: Takematsu, K]]	+	[[Category: Takematsu K]]
-	[[Category: Vlcek, A]]	+	[[Category: Vlcek A]]
-	[[Category: Williamson, H]]	+	[[Category: Williamson H]]
-	[[Category: Winkler~~, J R~~]]	+	[[Category: Winkler JR]]
-	[[Category: Zalis, S]]	+	[[Category: Zalis S]]
-	~~[[Category: Electron hopping]]~~	+
-	~~[[Category: Electron transport~~]]	+

OCA at 13:01, 25 December 2019

OCA — Wed, 25 Dec 2019 13:01:13 GMT

←Older revision		Revision as of 13:01, 25 December 2019
Line 1:		Line 1:

	==Azurin 122W/124W/126Re==		==Azurin 122W/124W/126Re==
-	<StructureSection load='6mjs' size='340' side='right' caption='[[6mjs]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='6mjs' size='340' side='right'caption='[[6mjs]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mjs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MJS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mjs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MJS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=REQ:(1,10+PHENANTHROLINE)-(TRI-CARBON+MONOXIDE)+RHENIUM+(I)'>REQ</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=REQ:(1,10+PHENANTHROLINE)-(TRI-CARBON+MONOXIDE)+RHENIUM+(I)'>REQ</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">azu, PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mjs OCA], [http://pdbe.org/6mjs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mjs RCSB], [http://www.ebi.ac.uk/pdbsum/6mjs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mjs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mjs OCA], [http://pdbe.org/6mjs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mjs RCSB], [http://www.ebi.ac.uk/pdbsum/6mjs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mjs ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 6mjs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6mjs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Azurin 3D structures\|Azurin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseae]]
	[[Category: Gray, H B]]		[[Category: Gray, H B]]
	[[Category: Heyda, J]]		[[Category: Heyda, J]]

OCA at 06:52, 21 February 2019

OCA — Thu, 21 Feb 2019 06:52:51 GMT

←Older revision		Revision as of 06:52, 21 February 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6mjs is ~~ON HOLD until Paper~~ Publication	+	==Azurin 122W/124W/126Re==
		+	<StructureSection load='6mjs' size='340' side='right' caption='[[6mjs]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mjs]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MJS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MJS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=REQ:(1,10+PHENANTHROLINE)-(TRI-CARBON+MONOXIDE)+RHENIUM+(I)'>REQ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mjs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mjs OCA], [http://pdbe.org/6mjs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mjs RCSB], [http://www.ebi.ac.uk/pdbsum/6mjs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mjs ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE]] Transfers electrons from cytochrome c551 to cytochrome oxidase.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have constructed and structurally characterized a Pseudomonas aeruginosa azurin mutant Re126WWCu(I) , where two adjacent tryptophan residues (W124 and W122, indole separation 3.6-4.1 A) are inserted between the Cu(I) center and a Re photosensitizer coordinated to the imidazole of H126 (Re(I)(H126)(CO)3(4,7-dimethyl-1,10-phenanthroline)(+)). Cu(I) oxidation by the photoexcited Re label (Re) 22.9 A away proceeds with a approximately 70 ns time constant, similar to that of a single-tryptophan mutant ( approximately 40 ns) with a 19.4 A Re-Cu distance. Time-resolved spectroscopy (luminescence, visible and IR absorption) revealed two rapid reversible electron transfer steps, W124 --> Re (400-475 ps, K 1 congruent with 3.5-4) and W122 --> W124(+) (7-9 ns, K 2 congruent with 0.55-0.75), followed by a rate-determining (70-90 ns) Cu(I) oxidation by W122(+) ca. 11 A away. The photocycle is completed by 120 mus recombination. No photochemical Cu(I) oxidation was observed in Re126FWCu(I) , whereas in Re126WFCu(I) , the photocycle is restricted to the ReH126W124 unit and Cu(I) remains isolated. QM/MM/MD simulations of Re126WWCu(I) indicate that indole solvation changes through the hopping process and W124 --> *Re electron transfer is accompanied by water fluctuations that tighten W124 solvation. Our finding that multistep tunneling (hopping) confers a approximately 9000-fold advantage over single-step tunneling in the double-tryptophan protein supports the proposal that hole-hopping through tryptophan/tyrosine chains protects enzymes from oxidative damage.

-	~~Authors:~~ Takematsu, K., Zalis, S., Gray~~, H.B.~~, Vlcek, A., Winkler~~, J~~.R.~~, Williamson, H~~.~~, Kaiser, J~~.T., ~~Heyda, J., Hollas, D~~.	+	Two Tryptophans Are Better Than One in Accelerating Electron Flow through a Protein.,Takematsu K, Williamson HR, Nikolovski P, Kaiser JT, Sheng Y, Pospisil P, Towrie M, Heyda J, Hollas D, Zalis S, Gray HB, Vlcek A, Winkler JR ACS Cent Sci. 2019 Jan 23;5(1):192-200. doi: 10.1021/acscentsci.8b00882. Epub, 2019 Jan 7. PMID:30693338<ref>PMID:30693338</ref>

-	~~Description: Azurin 122W~~/~~124W~~/~~126Re~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Hollas, D]]~~	+	<div class="pdbe-citations 6mjs" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Williamson~~, H]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gray, H B]]
	[[Category: Heyda, J]]		[[Category: Heyda, J]]
-	[[Category: ~~Gray~~, ~~H.B~~]]	+	[[Category: Hollas, D]]
-	[[Category: Kaiser, J.T]]	+	[[Category: Kaiser, J T]]
-	~~[[Category: Vlcek, A~~]]	+
	[[Category: Takematsu, K]]		[[Category: Takematsu, K]]
		+	[[Category: Vlcek, A]]
		+	[[Category: Williamson, H]]
		+	[[Category: Winkler, J R]]
	[[Category: Zalis, S]]		[[Category: Zalis, S]]
-	[[Category: ~~Winkler, J.R~~]]	+	[[Category: Electron hopping]]
		+	[[Category: Electron transport]]

OCA at 06:30, 17 October 2018

OCA — Wed, 17 Oct 2018 06:30:37 GMT

←Older revision		Revision as of 06:30, 17 October 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mjs is ON HOLD	+	The entry 6mjs is ON HOLD until Paper Publication

	Authors: Takematsu, K., Zalis, S., Gray, H.B., Vlcek, A., Winkler, J.R., Williamson, H., Kaiser, J.T., Heyda, J., Hollas, D.		Authors: Takematsu, K., Zalis, S., Gray, H.B., Vlcek, A., Winkler, J.R., Williamson, H., Kaiser, J.T., Heyda, J., Hollas, D.

OCA: Protected "6mjs" [edit=sysop:move=sysop]

OCA — Wed, 03 Oct 2018 06:27:03 GMT

Protected "6mjs" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:27, 3 October 2018

OCA: New page: '''Unreleased structure''' The entry 6mjs is ON HOLD Authors: Takematsu, K., Zalis, S., Gray, H.B., Vlcek, A., Winkler, J.R., Williamson, H., Kaiser, J.T., Heyda, J., Hollas, D. Descri...

OCA — Wed, 03 Oct 2018 06:27:02 GMT

New page: '''Unreleased structure''' The entry 6mjs is ON HOLD Authors: Takematsu, K., Zalis, S., Gray, H.B., Vlcek, A., Winkler, J.R., Williamson, H., Kaiser, J.T., Heyda, J., Hollas, D. Descri...

New page

'''Unreleased structure'''

The entry 6mjs is ON HOLD

Authors: Takematsu, K., Zalis, S., Gray, H.B., Vlcek, A., Winkler, J.R., Williamson, H., Kaiser, J.T., Heyda, J., Hollas, D.

Description: Azurin 122W/124W/126Re
[[Category: Unreleased Structures]]
[[Category: Hollas, D]]
[[Category: Williamson, H]]
[[Category: Heyda, J]]
[[Category: Gray, H.B]]
[[Category: Kaiser, J.T]]
[[Category: Vlcek, A]]
[[Category: Takematsu, K]]
[[Category: Zalis, S]]
[[Category: Winkler, J.R]]