6mna - Revision history

OCA at 06:34, 11 October 2023

OCA — Wed, 11 Oct 2023 06:34:49 GMT

←Older revision		Revision as of 06:34, 11 October 2023
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	<StructureSection load='6mna' size='340' side='right'caption='[[6mna]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='6mna' size='340' side='right'caption='[[6mna]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6mna]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MNA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6MNA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6mna]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MNA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=T6D:alpha~~-~~D-glucopyranosyl+6-O-decanoyl-alpha-D-glucopyranoside'>T6D</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~lpqN, Rv0583c ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id~~=~~83332 MYCTU])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900093:6-decanoyl-trehalose'>PRD_900093</scene>, <scene name='pdbligand=U2D:6-O-decanoyl-alpha-D-glucopyranose'>U2D</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6mna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mna OCA], [~~http~~://pdbe.org/6mna PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6mna RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6mna PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6mna ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mna OCA], [https://pdbe.org/6mna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mna RCSB], [https://www.ebi.ac.uk/pdbsum/6mna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mna ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LPQN_MYCTU LPQN_MYCTU] Involved in cell envelope biogenesis. May act as a membrane fusion protein, connecting MmpL transporters with periplasmic proteins, and play a role in cell envelope lipid changes during biofilm maturation.<ref>PMID:31471317</ref> Is also a virulence factor required for intracellular survival (PubMed:30118682). Associates with CBL, a host ubiquitin ligase, and probably blocks the normal functions of CBL and disturbs CBL-mediated antibacterial activity (PubMed:30118682). Interaction counteracts antibacterial defense but causes a reciprocal enhancement of antiviral defense (PubMed:30118676, PubMed:30118682).<ref>PMID:30118682</ref> <ref>PMID:30118676</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Rajavel, M]]	+	[[Category: Rajavel M]]
-	[[Category: Su~~, C C~~]]	+	[[Category: Su CC]]
-	[[Category: Yu~~, E W]]~~	+	[[Category: Yu EW]]
-	~~[[Category: Lipid binding protein]]~~	+
-	~~[[Category: Trehalose 6-dodecanoate~~]]	+

OCA at 08:25, 6 November 2019

OCA — Wed, 06 Nov 2019 08:25:42 GMT

←Older revision		Revision as of 08:25, 6 November 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6mna is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacterium tuberculosis==
		+	<StructureSection load='6mna' size='340' side='right'caption='[[6mna]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6mna]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MNA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MNA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=T6D:alpha-D-glucopyranosyl+6-O-decanoyl-alpha-D-glucopyranoside'>T6D</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpqN, Rv0583c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mna OCA], [http://pdbe.org/6mna PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mna RCSB], [http://www.ebi.ac.uk/pdbsum/6mna PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mna ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mycobacterial cell envelope is crucial to host-pathogen interactions as a barrier against antibiotics and the host immune response. In addition, cell envelope lipids are mycobacterial virulence factors. Cell envelope lipid biosynthesis is the target of a number of frontline tuberculosis treatments and has been the focus of much research. However, the transport mechanisms by which these lipids reach the mycomembrane remain poorly understood. Many envelope lipids are exported from the cytoplasm to the periplasmic space via the mycobacterial membrane protein large (MmpL) family of proteins. In other bacteria, lipoproteins can contribute to outer membrane biogenesis through direct binding of substrates and/or protein-protein associations with extracytoplasmic biosynthetic enzymes. In this report, we investigate whether the lipoprotein LpqN plays a similar role in mycobacteria. Using a genetic two-hybrid approach, we demonstrate that LpqN interacts with periplasmic loop domains of the MmpL3 and MmpL11 transporters that export mycolic acid-containing cell envelope lipids. We observe that LpqN also interacts with secreted cell envelope biosynthetic enzymes such as Ag85A via pulldown assays. The X-ray crystal structures of LpqN and LpqN bound to dodecyl-trehalose suggest that LpqN directly binds trehalose monomycolate, the MmpL3 and Ag85A substrate. Finally, we observe altered lipid profiles of the DeltalpqN mutant during biofilm maturation, pointing toward a possible physiological role for the protein. The results of this study suggest that LpqN may act as a membrane fusion protein, connecting MmpL transporters with periplasmic proteins, and provide general insight into the role of lipoproteins in Mycobacterium tuberculosis cell envelope biogenesis.

-	~~Authors: Rajavel~~, M., Su~~, C.C.~~, Yu, E.W.	+	Structural and functional evidence that lipoprotein LpqN supports cell envelope biogenesis in Mycobacterium tuberculosis.,Melly GC, Stokas H, Dunaj JL, Hsu FF, Rajavel M, Su CC, Yu EW, Purdy GE J Biol Chem. 2019 Oct 25;294(43):15711-15723. doi: 10.1074/jbc.RA119.008781. Epub, 2019 Aug 30. PMID:31471317<ref>PMID:31471317</ref>

-	~~Description: Crystal structure~~ of ~~LpqN involved in cell envelope biogenesis~~ of ~~Mycobacterium tuberculosis~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6mna" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Rajavel, M]]		[[Category: Rajavel, M]]
-	[[Category: Yu, E.W]]	+	[[Category: Su, C C]]
-	[[Category: ~~Su, C.C~~]]	+	[[Category: Yu, E W]]
		+	[[Category: Lipid binding protein]]
		+	[[Category: Trehalose 6-dodecanoate]]

OCA at 06:33, 17 October 2018

OCA — Wed, 17 Oct 2018 06:33:28 GMT

←Older revision		Revision as of 06:33, 17 October 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6mna is ON HOLD	+	The entry 6mna is ON HOLD until Paper Publication

	Authors: Rajavel, M., Su, C.C., Yu, E.W.		Authors: Rajavel, M., Su, C.C., Yu, E.W.
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	Description: Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacterium tuberculosis		Description: Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacterium tuberculosis
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Su, C.C]]
	[[Category: Rajavel, M]]		[[Category: Rajavel, M]]
	[[Category: Yu, E.W]]		[[Category: Yu, E.W]]
		+	[[Category: Su, C.C]]

OCA: Protected "6mna" [edit=sysop:move=sysop]

OCA — Wed, 10 Oct 2018 06:34:07 GMT

Protected "6mna" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:34, 10 October 2018

OCA: New page: '''Unreleased structure''' The entry 6mna is ON HOLD Authors: Rajavel, M., Su, C.C., Yu, E.W. Description: Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacteri...

OCA — Wed, 10 Oct 2018 06:34:06 GMT

New page: '''Unreleased structure''' The entry 6mna is ON HOLD Authors: Rajavel, M., Su, C.C., Yu, E.W. Description: Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacteri...

New page

'''Unreleased structure'''

The entry 6mna is ON HOLD

Authors: Rajavel, M., Su, C.C., Yu, E.W.

Description: Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacterium tuberculosis
[[Category: Unreleased Structures]]
[[Category: Su, C.C]]
[[Category: Rajavel, M]]
[[Category: Yu, E.W]]